Effects of Low-Intensity Pulsed Ultrasound on the Expression of Cyclooxygenase-2 in Mandibular Condylar Chondrocytes

Tanimoto, Kotaro; Tanne, Yuki; Inubushi, Toshihiro; Kamiya, Takashi; Kunimatsu, Ryo; Hirose, Naoto; Mitsuyoshi, Tomomi; Su, Shao-Ching; Tanaka, Eiji; Tanne, Kazuo

doi:10.11607/ofph.1156

Cited by 32 publications

(19 citation statements)

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“…Ito et al, have shown significant inhibition of MMP13 and MMP1 mRNA expression in LIPUS treated articular cartilage in the presence of IL-1β 29 . LIPUS stimulation also significantly reduced IL-1β induced expression of Cox-2 in mandibular condylar chondrocytes through inhibition of IL-1β activated ERK1/2 phosphorylation 39 . LIPUS at an intensity of 30mW/cm 2 for 20 min/day has been shown to significantly increase osteogenic differentiation, osteoblast proliferation, and bone healing in both in-vitro and in vivo models 26,40 .…”

Section: Discussionmentioning

confidence: 84%

Chondro-protective effects of low intensity pulsed ultrasound

Uddin

Richbourgh

Ding

et al. 2016

Osteoarthritis and Cartilage

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Objectives Cartilage is a highly mechano-responsive tissue. Chondrocytes undergo a series of complex changes, including proliferation and metabolic alteration as the target of external biomechanical and biochemical stimuli. IL-1β is known to regulate chondrocyte metabolism and play an important role in the pathogenesis of osteoarthritis. The objective of this study was to employ low-intensity pulsed ultrasound (LIPUS) as a localized mechanical stimulus and assess its effects on chondrocyte migration, proliferation, metabolism, and differentiation, as well as its ability to suppress IL-1β mediated catabolism in cartilage. Methods Human cartilage explants and chondrocytes were stimulated by LIPUS in presence and absence of of IL-1β to asses cartilage degradation, chondrocytes metabolism, migration and proliferation. Western blot analyses were conducted to study IL-1β the associated NFκB pathway in chondrocytes. Results LIPUS stimulation increased the proteoglycan content in human cartilage explants and inhibited IL-1β induced loss of proteoglycans. LIPUS stimulation increased rates of chondrocyte migration and proliferation, and promoted chondrogenesis in mesenchymal stem cells. Further, LIPUS suppressed IL-1β induced activation of phosphorylation of NFκB-p65 and IκBα leading to reduced expression of MMP13 and ADAMT5 in chondrocytes. Conclusions Collectively, these data demonstrate the potential therapeutic effects of LIPUS in preventing cartilage degradation and treating osteoarthritis via a mechanical stimulation that inhibits the catabolic action of IL-1β and stimulates chondrocyte migration, proliferation, and differentiation.

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Section: Discussionmentioning

confidence: 84%

Chondro-protective effects of low intensity pulsed ultrasound

Uddin

Richbourgh

Ding

et al. 2016

Osteoarthritis and Cartilage

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“…[7][8][9][10] Several studies explored potential treatments for OIIRR [11][12][13][14] but the only reported clinically acceptable modality is low-intensity pulsed ultrasound (LIPUS). 15 LIPUS is acoustic pressure waves that can stimulate a variety of cells including cementoblasts, 16 odontoblasts, 17 osteoblasts, 18 chondrocytes, 19 gingival cells, 20,21 and periodontal ligament cells. 22 Previous studies investigating the effects of LIPUS on OIRR have reported that LIPUS promotes cementogenesis by increasing the Alkaline phosphate (ALP) activity, 16,23 collagen-I synthesis, 16 and Runx-2 protein 16 in cementoblasts and inhibits cementoclastogenesis 24,25 In addition, a previous study 15 reported that LIPUS reduced the severity of OIRR due to tipping-type orthodontic tooth movement and promoted cementum repair.…”

Section: Introductionmentioning

confidence: 99%

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

Raza

Major

Dederich

et al. 2015

The Angle Orthodontist

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Objectives: To evaluate the effects of low-intensity pulsed ultrasound (LIPUS) on orthodontically induced tooth root resorption caused by torque in human subjects. Materials and Methods: Ten healthy patients (12-35 years of age) who required extraction of all first premolars as a part of their routine orthodontic treatment were recruited. A 15u twist was applied in the arch wire using 0.019 3 0.025-inch TMA in a 0.022-inch bracket system (Synergy R) that produced a buccal root torque of approximately 5 N/mm at the bracket level. Using a split mouth design, randomization, and blinding, one side of the arch received LIPUS for 20 minutes per day for 4 weeks at an incident intensity of 30 mW/cm 2 of the transducers' surface area. The other side served as a self-control, which received a sham transducer. After 4 weeks, all first premolars were extracted and micro-computed tomographic analysis was performed on these extracted teeth. A linear mixed-model statistical analysis was used. Results: LIPUS-treated teeth showed significantly less total volume of resorption lacunae compared to control teeth by a mean difference of (0.54 6 0.09 mm 3 ) (P , .001) and percentage of root resorption by a mean difference of (0.33 6 0.05 mm 3 ) (P , .001). In addition, significantly fewer resorption lacunae were found on all root surfaces in the LIPUS group compared to the control except in the instance of the distal surface. Limitations: This study was performed on limited number of cases during a 4-week period. Conclusions: LIPUS minimizes root resorption when applied during torque tooth movement over a 4-week period. (Angle Orthod. 2016;86:550-557.)

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“…However, because the chondrocytic mechanotransducing processes initiated or forged by LIPUS may be time sensitive, research to examine an array of timing parameters and/or possible time frames for optimally modulating favorable LIPUS associated outcomes and avoiding unfavorable ones is strongly recommended. As well, examining the relative impact of other LIPUS stimulation parameters as this affects the sensitivity of the chondrocyte membrane and its receptors in both health and disease may help to shed new light on LIPUS and its potential for fostering desirable repair of cartilage defects, especially Figure 1 Schematic representation of the impact of LIPUS on chondrocyte functions that maybe useful for treating osteoarthritis symptoms, and for regenerative cartilage repair efforts as adapted from [5,11,16,19,21,25,26,29,46,47,50,54,63,64,[66][67][68][69][70] osteoarticular cartilage reparative and protective processes. In addition, efforts to carefully examine the impact of LIPUS on multiple chondrocyte cell membrane signaling pathways, and target genes, especially those not previously examined, is strongly encouraged for further examining treatment mechanisms, as is research to examine the utility of matching the frequency of the driving force of LIPUS to that of the chondrocyte system's natural frequency of vibration as outlined by Miller et al [51,52] , and Louw et al [68] .…”

Section: Discussionmentioning

confidence: 99%

“…These positive LIPUS induced effects include, but are not limited to, its ability to significantly attenuate the impact of destructive chondrolytic enzymes known to destroy cartilage matrix in injured joints [46] , in a dose dependent manner in the context of cultured chondrocytes and articular cartilage explants [47] , whose effect is greater in early ratherthan late osteoarthritis [17] . On the other hand, LIPUS not only attenuates the production of an array of damaging catabolic responses associated with the natural history of osteoarthritis [27] , but its thoughtful application is shown to concurrently induce favorable anabolic chondrocyte responses, as shown by associated changes in the extracellular matrix protein content and the parallel upregulation of chondrogenic genes [32] .…”

Section: Sekino Et Al[3]mentioning

confidence: 99%

Low Intensity Pulsed Ultrasound and Articular Cartilage Repair

Marks

2019

International Journal of Orthopaedics

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Low intensity pulsed ultrasound [LIPUS], a physical modality that has demonstrated utility in bone healing contexts including non union fracture situations has been proposed to also impact articular cartilage chondrocyte cell metabolism in a favorable way. This current review focuses on two questions: a) whether LIPUS produces any clinically significant impact on chondrocyte structure or function; b) whether the impacts observed support a role for LIPUS in efforts to foster cartilage regeneration and healing applications. Drawn from the English language literature published over the last 35 years, the majority of the available basic or preclinical studies presently reviewed demonstrate LIPUS applications to consistently yield favorable cartilage chondrogenic responses, regardless of substrate, and to be worthy of further exploration in the clinical realm for promoting much sought

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Effects of Low-Intensity Pulsed Ultrasound on the Expression of Cyclooxygenase-2 in Mandibular Condylar Chondrocytes

Cited by 32 publications

References 0 publications

Chondro-protective effects of low intensity pulsed ultrasound

Chondro-protective effects of low intensity pulsed ultrasound

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

Low Intensity Pulsed Ultrasound and Articular Cartilage Repair

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