Effects of low-frequency ultrasound on microcirculation in venous leg ulcers

Wollina, Uwe; Heinig, Birgit; Naumann, G; Scheibe, Armin; Schmidt, W.; Neugebauer, Reimund

doi:10.4103/0019-5154.80412

Cited by 15 publications

(10 citation statements)

References 22 publications

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“…Continuous 1-MHz low-intensity ultrasound was able to affect the fragile and leaky angiogenic blood vessels in a tumor (5)., and 3-MHz ultrasound was more efficacious than 1 MHz for antivascular cancer therapy, in which direct heating of the tumor due to ultrasound absorption is the main mechanism (6). However, in low-frequency ultrasound (7), it is ultrasoundinduced cavitation that may play an important role in the antitumor treatment (8). Collapse cavitation causes capillary destruction and, thus, may be therapeutically beneficial for tumor (9).…”

Section: Introductionmentioning

confidence: 99%

Effects of low-frequency ultrasound and microbubbles on angiogenesis-associated proteins in subcutaneous tumors of nude mice

Shen¹,

Shen²,

Ji-zhen³

et al. 2013

Oncology Reports

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It has been shown that 1 and 3 MHz low-intensity ultrasound was able to affect the fragile and leaky angiogenic blood vessels in a tumor. However, the biological effects of 21 kHz low-intensity ultrasound on tumors remain unclear. The aim of the present study was to explore the effects of 21 kHz ultrasound with microbubbles on the regulation of vascular endothelial growth factor (VEGF), cyclooxygenase-2 (COX-2) and apoptosis in subcutaneous prostate tumors in nude mice. The study included three parts, each with 20 tumor-bearing nude mice. Twenty nude mice were divided into four groups: control (sham treatment), microbubble ultrasound contrast agent (UCA), low-frequency ultrasound (US) and US+UCA groups. The UCA used was a microbubble contrast agent (SonoVue). The parameter of ultrasound: 21 kHz, an intensity of 26 mW/cm2, 40% duty cycle (on 2 sec, off 3 sec), 3 min, once every other day for 2 weeks. In the first study, all subcutaneous tumors were examined by contrast-enhanced ultrasonography (CEUS) at the initiation and completion of the experiments. Peak intensity (PI), time to peak intensity (TTP) and area under the curve (AUC) on the time intensity curve (TIC) were analyzed. In the second study, the intensity of VEGF and COX-2 protein expression in the vascular endothelium and cytoplasm was evaluated using immunohistochemistry and laser confocal microscopy. In the third study, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay was used for the evaluation of cell apoptosis in tumor tissues. The tumor cells and vasculature were examined by transmission electron microscopy (TEM). Only in the US+UCA group, PI and AUC decreased. The intensity of COX-2 and VEGF in the US+UCA group in immunohistochemical staining and laser confocal microscopy was lower compared to that of the other three groups. More cell apoptosis was found in the US+UCA group compared to the other 3 groups. In the control, UCA and US groups, the tumors had intact vascular endothelium and vessel lumens in TEM. However, lumen occlusion of vessels was observed in the US+UCA group. Twenty-one kHz low-intensity ultrasound with microbubbles may have anti-angiogenic effects on subcutaneous tumors in nude mice.

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Section: Introductionmentioning

confidence: 99%

Effects of low-frequency ultrasound and microbubbles on angiogenesis-associated proteins in subcutaneous tumors of nude mice

Shen¹,

Shen²,

Ji-zhen³

et al. 2013

Oncology Reports

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“…longer treatment (45 minutes of 20 kHz of ultrasound) or treatment at higher frequency (15 minutes of 100 kHz ultrasound) 10. Work by Wollina et al11 also found superior healing with treatment frequency at the lower end of the spectrum (34 kHz was superior to 53.5 and 75 kHz and significantly increased oxygen saturation and superficial hemoglobin concentration in 12 patients). Treatment of residual burn wounds every other day with 25 kHz low-frequency ultrasound over a period of 2 weeks resulted in 100% wound healing and increased healing rate 12.…”

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confidence: 97%

Low-Frequency Ultrasound Debridement in Chronic Wound Healing: A Systematic Review of Current Evidence

2017

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Chronic wounds are painful and debilitating to patients, pose a clinical challenge to physicians, and impose financial burden on the health-care system. New treatment options are therefore highly sought after. Ultrasound debridement is a promising technology that functions to disperse bacterial biofilms and stimulate wound healing. In this review, we focus on low-frequency ultrasound (20-60 kHz) and summarize the findings of 25 recent studies examining ultrasound efficacy. Ultrasound debridement appears to be most effective when used 3 times a week and has the potential to decrease exudate and slough, decrease patient pain, disperse biofilms, and increase healing in wounds of various etiology. Although current studies are generally of smaller size, the results are promising and we recommend the testing of low-frequency ultrasound therapy in clinical practice on a larger scale.

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“…Chemically reactive species are also effective as anti-biofilm agents in the external application of atmospheric cold plasma against biofilms ( [28]; Table 1). Low frequency ultrasonic therapy, clinically applied to improve chronic wounds, has been shown effective against biofilm-forming microorganisms in experimental infections, probably through the generation of mechanical forces [29][30][31]. It is worth noting, though, that any successful antimicrobial therapy needs to be supported by a functional immune system to eradicate the foci of infection.…”

Section: Treatment Strategies For Biofilm Infectionsmentioning

confidence: 99%

Biofilm formation – what we can learn from recent developments

et al. 2018

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Although biofilms have been observed early in the history of microbial research, their impact has only recently been fully recognized. Biofilm infections, which contribute to up to 80% of human microbial infections, are associated with common human disorders, such as diabetes mellitus and poor dental hygiene, but also with medical implants. The associated chronic infections such as wound infections, dental caries and periodontitis significantly enhance morbidity, affect quality of life and can aid development of follow-up diseases such as cancer. Biofilm infections remain challenging to treat and antibiotic monotherapy is often insufficient, although some rediscovered traditional compounds have shown surprising efficiency. Innovative anti-biofilm strategies include application of anti-biofilm small molecules, intrinsic or external stimulation of production of reactive molecules, utilization of materials with antimicrobial properties and dispersion of biofilms by digestion of the extracellular matrix, also in combination with physical biofilm breakdown. Although basic principles of biofilm formation have been deciphered, the molecular understanding of the formation and structural organization of various types of biofilms has just begun to emerge. Basic studies of biofilm physiology have also resulted in an unexpected discovery of cyclic dinucleotide second messengers that are involved in interkingdom crosstalk via specific mammalian receptors. These findings even open up new venues for exploring novel anti-biofilm strategies.

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Effects of low-frequency ultrasound on microcirculation in venous leg ulcers

Cited by 15 publications

References 22 publications

Effects of low-frequency ultrasound and microbubbles on angiogenesis-associated proteins in subcutaneous tumors of nude mice

Effects of low-frequency ultrasound and microbubbles on angiogenesis-associated proteins in subcutaneous tumors of nude mice

Low-Frequency Ultrasound Debridement in Chronic Wound Healing: A Systematic Review of Current Evidence

Biofilm formation – what we can learn from recent developments

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