Effects of low-dose gamma radiation on DNA damage, chromosomal aberration and expression of repair genes in human blood cells

Sudprasert, Wanwisa; Navasumrit, Panida; Ruchirawat, Mathuros

doi:10.1016/j.ijheh.2006.06.004

Cited by 101 publications

(55 citation statements)

References 48 publications

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“…34,35,36,37 In that manner, standard alkaline and Fpg-modified version of the comet assay were used as well as the DNA diffusion test to access whether this type of radiation induces apoptotic or necrotic cell death. Techniques used in this study showed genotoxic effect of gamma rays on DNA molecule of peripheral blood human lym- phocytes in vitro.…”

Section: Comet Assay and Fpg-modified Comet Assaymentioning

confidence: 99%

Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation

Mikloš¹,

Gajski²,

Garaj‐Vrhovac³

2009

Radiology and Oncology

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Section: Comet Assay and Fpg-modified Comet Assaymentioning

confidence: 99%

Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation

Mikloš¹,

Gajski²,

Garaj‐Vrhovac³

2009

Radiology and Oncology

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“…In previous studies, gamma radiation has diverse effects on proliferation, DNA repair, release of cytokines and etc. in in vitro cellular as well as in vivo models [16][17][18][19][20] . Especially, high gamma radiation increases calcium-induced keratinocyte HaCaT cells were exposed to gamma radiation and control cells were untreated.…”

Section: Resultsmentioning

confidence: 99%

170 Single low-dose radiation induced regulation of keratinocyte differentiation in calcium-induced HaCaT cells

Hahn

Lee

Choi

et al. 2017

Journal of Investigative Dermatology

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Background: We are continually exposed to low-dose radiation (LDR) in the range 0.1 Gy from natural sources, medical devices, nuclear energy plants, and other industrial sources of ionizing radiation. There are three models for the biological mechanism of LDR: the linear no-threshold model, the hormetic model, and the threshold model. Objective: We used keratinocytes as a model system to investigate the molecular genetic effects of LDR on epidermal cell differentiation. Methods: To identify keratinocyte differentiation, we performed western blots using a specific antibody for involucrin, which is a precursor protein of the keratinocyte cornified envelope and a marker for keratinocyte terminal differentiation. We also performed quantitative polymerase chain reaction. We examined whether LDR induces changes in involucrin messenger RNA (mRNA) and protein levels in calcium-induced keratinocyte differentiation. Results: Exposure of HaCaT cells to LDR (0.1 Gy) induced p21 expression. p21 is a key regulator that induces growth arrest and represses stemness, which accelerates keratinocyte differentiation. We correlated involucrin expression with keratinocyte differentiation, and examined the effects of LDR on involucrin levels and keratinocyte development. LDR significantly increased involucrin mRNA and protein levels during calcium-induced keratinocyte differentiation. Conclusion: These studies provide new evidence for the biological role of LDR, and identify the potential to utilize LDR to regulate or induce keratinocyte differentiation. (Ann Dermatol 28(4) 433∼

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“…6,[14][15][16] Their results indicated that 125 I in close proximity to DNA reduced the Double-Strand Breaks per decay by a direct mechanism. The extent of the damaged DNA due to irradiation is dependent on the distance between the decaying 125 I atom and the cleaved deoxyribosyl unit.…”

mentioning

confidence: 98%

“…The extent of the damaged DNA due to irradiation is dependent on the distance between the decaying 125 I atom and the cleaved deoxyribosyl unit. 15,16 The Auger electrons emitted from 125 I deposit their all energy within several nanometers distance and their simultaneous action produces a considerable amount of damage to biomolecules, compared with that of an a particle. 17 The Auger cascade would only produce disruption of *5 base pairs on both sides of the decaying atom if the 125 I was affixed to a base that was incorporated into a duplex deoxyribonucleic oligonucleotide.…”

mentioning

confidence: 99%

Characteristic Variation of α-Fetoprotein DNA Nanometer-Range Irradiated by Iodine-125

Cheng

Huang²,

Li³

et al. 2013

Cancer Biotherapy and Radiopharmaceuticals

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To obtain the characteristic variation of structure and functional groups of a-fetoprotein (AFP) DNA irradiated by iodine-125( 125 I), the AFP antisense oligonucleotide labeled with various radioactivity dose 125 I was mixed with the AFP DNA in a simulated polymerase chain reaction temperature condition. After the mixtures were irradiated by the 125 I from 2 to 72 hours, the mutation of the biogenic conformation and functional groups of the irradiated DNA were investigated using laser Raman spectroscopy. The shifted peak and the decreased intensity of the characteristic Raman spectra were found, which demonstrated that the structure of the phosphodiester linkage was broke, the pyridine and purine bases in DNA emerged and damaged. The model of gene conformation changed from form B to form C spectrum after the nanometer-range irradiation with 125 I from 2 to 24 hours. The damage of local pyridine and purine bases gradually increased along with the accumulation of irradiation, and the bases and ribosome were finally dissociated and stacked.

show abstract

Effects of low-dose gamma radiation on DNA damage, chromosomal aberration and expression of repair genes in human blood cells

Cited by 101 publications

References 48 publications

Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation

Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation

170 Single low-dose radiation induced regulation of keratinocyte differentiation in calcium-induced HaCaT cells

Characteristic Variation of α-Fetoprotein DNA Nanometer-Range Irradiated by Iodine-125

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