Effects of long‐term intermittent versus chronic calorie restriction on oxidative stress in a mouse cancer model

Cicekdal, Munevver Burcu; Tuna, Bilge Güvenç; Charehsaz, Mohammad; Cleary, Margot P.; Aydın, Ahmet; Doğan, Soner

doi:10.1002/iub.2145

Cited by 12 publications

(7 citation statements)

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“…[17] Thus, it is anticipated that the role of elevated adiponectin in protection from mammary tumorigenesis or the role of reduced adiponectin in obesity in enhancing MT development can now be investigated in detail using the mouse model in the current study since cancer development in human is more complex. [26][27][28] Although these findings lead us to conclude that AdipoR1, rather than AdipoR2, might be dominant in adiponectin signaling in MT development, further studies are necessary to characterize the roles of these two receptors in tumor development.…”

Section: Discussionmentioning

confidence: 99%

“…The mice were obtained using the breeding protocol and genotyping assay previously described and fed the AIN-93M diet (Harlan Teklad, Madison, WI) from eight until 74 weeks of age. [17] Over the course of the experiment, serum samples were obtained via orbital bleeding at 10,19,25,28,40,49 and 74 weeks of age. Then, mice were sacrificed, dissected and liver tissues, MFP, or suspected MT were collected.…”

Section: Methodsmentioning

confidence: 99%

“…Blood collection was performed five hours after animals were given the amount of their daily food at all time points. To determine if changes in serum adiponectin preceded MT development, serum samples were obtained at multiple age points (10,19,25,28,40, 49 and 74 weeks old). Adiponectin levels in serum were measured with a commercial RIA kit per manufacturer's protocol (LINCO Research, MO).…”

Section: Measurement Of Serum Adiponectin Levelsmentioning

confidence: 99%

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Roles of Adiponectin Signaling Related Proteins in Mammary Tumor Development

Tuna

Cleary

Doğan

2019

Laparosc Endosc Surg Sci

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This study aims to investigate the expression levels of adiponectin signaling related proteins in mammary tissue, liver and breast cancer tissue in mice. Adiponectin, an adipocytokine, is secreted from adipose tissue and has been documented to have roles in diabetes, inflammation, and cancer development. In particular, levels of serum adiponectin are inversely associated with obesity and a decrease in serum adiponectin levels have been reported to be associated with breast cancer. There are two adiponectin receptor subtypes, AdipoR1 and AdipoR2, which have been identified in mammalian tissues, including human cancer cell lines and also in human mammary tumors. However, the role of adiponectin receptors in breast cancer development remains to be established. Methods: In this study, MMTV-TGF-a transgenic mice were fed from week 10 up to week 74 of age. Expression levels of adiponectin, AdipoR1 and AdipoR2 proteins were measured in the mammary fat pad (MFP), mammary tumor (MT) and liver tissues from 74 weeks old MMTV-TGF-a transgenic mice with and without MT using Western Blot. Adiponectin levels were measured using ELISA assay. Results: Protein expression levels of Adiponectin and AdipoR1 were significantly lower in MTs compared to control tissues. However, AdipoR2 protein expression levels were similar in MT and MFP tissues from MT-positive and MT-negative mice. The expression levels of adiponectin, AdipoR1 and AdipoR2 proteins in liver tissues were also similar in MT-positive and MT-negative mice. Serum adiponectin levels of the MT-positive and MT-negative mice were similar. Conclusion: These results indicate that adiponectin and its receptors are differentially regulated depending upon the specific tissue analyzed. AdipoR1 and adiponectin may play important roles in MT development.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Measurement Of Serum Adiponectin Levelsmentioning

confidence: 99%

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Roles of Adiponectin Signaling Related Proteins in Mammary Tumor Development

Tuna

Cleary

Doğan

2019

Laparosc Endosc Surg Sci

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“…The severity of the consequences of this imbalance depends on a variety of factors, including the level and the location of ROS production, the efficacy of antioxidant systems, and the cellular targets of ROS [11]. Aging and age-related diseases such as cancer, neurodegeneration, cardiovascular diseases, and diabetes have been attributed to oxidative stress [12][13][14]. Therefore, it is crucial to modulate intracellular ROS levels by the antioxidant defense mechanisms for the maintenance of redox homeostasis.…”

Section: Introductionmentioning

confidence: 99%

“…The health status of the mice was checked by an expert veterinarian regularly, at least once a week. Female mice were randomly assigned into three groups at 10 weeks of mouse age: ad libitum (AL), chronic CR (CCR), or intermittent CR (ICR) [12]. Mice diets (Altromin TPF1414) were purchased from Kobay AS (Ankara, Turkey).…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Calorie Restriction Protocols on Oxidative Stress Parameters in a Transgenic Mouse Model of Breast Cancer

Cicekdal¹,

Thomas²,

Tuna³

et al. 2022

Cureus

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Aging and diseases related to aging, such as cancer, have been linked to oxidative stress. On the other hand, calorie restriction (CR) is one of the most effective interventions to slow down aging and prevent a variety of diseases such as cancer in preclinical models. CR has also been reported to modify oxidative stress. The aim of this study was to investigate the effects of different CR protocols and aging on oxidative stress parameters in the MMTV-TGF-α breast cancer mouse model in a cross-sectional study. Female mice were randomly enrolled in three groups: ad libitum (AL), chronic calorie restriction (CCR, 15% CR) or intermittent calorie restriction (ICR, three weeks AL followed by one week 60% CR in cyclic periods) starting at the age of 10 weeks until 81/82 weeks of age. Liver samples were analyzed for malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels. At week 49/50, the GSH level increased significantly in the CCR group compared to the AL and ICR-R groups which had higher mammary tumor (MT) incidence rates. Additionally, liver MDA levels in ICR groups were significantly increased, while aging led to decreased CAT and SOD activities in all CR groups. The application of different CR protocols did not have any significant effect on MDA, CAT, and SOD parameters in the liver at week 81/82. These results suggest that although GSH may interfere with MT development at the systemic level, many of the oxidative stress parameters may have more local effects on tumor development than the systemic effects.

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