Effects of long-term fertilization on AM fungal community structure and Glomalin-related soil protein in the Loess Plateau of China

Abstract

Arbuscular mycorrhizal fungi (AMF) are crucial for ecosystem functioning, and thus have potential use for sustainable agriculture. In this study, we investigated the impact of organic and mineral fertilizers on the AMF community composition and content of Glomalin-related soil protein (GRSP) in a field experimental station which was established in 1979, in the Loess Plateau of China. Roots and soils were sampled three times during the growing period of winter wheat in 2008. The treatments including: N (inorganic N), NP (inorganic N and P), SNP (straw, inorganic N and P), M (farmyard manure), MNP (farmyard manure, inorganic N and P), and CK (no fertilization). AMF communities of root and soil samples were analyzed using PCR-DGGE, cloning and sequencing techniques; and GRSP content was determined by Bradford assay. Our results indicated that spore density, GRSP, and AMF community varied significantly in soils of long-term fertilization plots at three different wheat growing stages. The effects of wheat growing period on AMF community in roots were much more evident than fertilization regimes.

Dynamics of arbuscular mycorrhizal fungal community structure and functioning along a nitrogen enrichment gradient in an alpine meadow ecosystem

“…). Changes in AM fungal community structure caused by nutrient enrichment are often attributed to shifts in plant community composition (Liu et al ., ) and variation in soil characteristics (Wu et al ., ). This mechanism may be operating in our case, because the changes in AM fungal community composition in mixed roots were strongly associated with both plant species richness and soil available N concentration (Fig.…”

“…2). Changes in AM fungal community structure caused by nutrient enrichment are often attributed to shifts in plant community composition and variation in soil characteristics (Wu et al, 2011). This…”

Advances and Perspectives to Improve the Phosphorus Availability in Cropping Systems for Agroecological Phosphorus Management

“…In high-P soils, AMF communities proved to be different from those existing in low-P environments (Jiao et al, 2011). Even in high-P soils, rich AMF communities can be observed (Vestberg et al, 2011), although negative effect of high fertilization have also been reported (Wu et al, 2011). A negative relation between root hair length and AM colonization was reported in P-deficient barley, suggesting allocation of resources toward root hairs rather than AMF (Brown et al, 2013).…”

Diversity, richness and degree of colonization of arbuscular mycorrhizal fungi in coconut cultivated along with intercrops in high productive zone of Kerala, India

“…In maize monoculture a very low number of AM fungal spores were reported as compared to grassland or a poplar grove (Bedini et al 2007). AMF species richness and community structure are determined by distinct processes in terrestrial ecosystems such as habitat mosaic (De Carvalho et al 2012), soil characteristics (Santos-González et al 2011;Ji et al 2012), long term fertilization (Wu et al 2011), invasive plants ( Barto et al 2011), tree-based intercropping (Bainard et al 2011) and distinct land use systems (Sturmer and Siqueira 2011). The availabilities of soil N and P might play key roles in determining the abundance and diversity of AM fungi (Liu et al 2009(Liu et al , 2012Alguacil et al 2010).…”