Effects of long-term fertilization on phoD-harboring bacterial community in Karst soils

Hu, Yajun; Xia, Yinhang; Sun, Qiaoyan; Liu, Kunping; Chen, Xiangbi; Ge, Tida; Zhu, Baoli; Zhu, Zhenke; Zhang, Zhenhua; Su, Yirong

doi:10.1016/j.scitotenv.2018.01.314

Cited by 148 publications

(89 citation statements)

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“…The Pb immobilization efficiency was significantly correlated with soil available P and WSP in this study, which is similar with the findings that Pb immobilized amount and available phosphorus present significant correlation (Park et al, 2011;Yuan et al, 2017). Additionally, some studies have reported that the addition of inorganic phosphorus can increase the abundance of P-cyclingrelated gene (Silva et al, 2017;Hu et al, 2018;Wei et al, 2019), and alter bacterial community composition (Lagos et al, 2016;Luo et al, 2017;Wei et al, 2019). However, the effect of PSB addition on indigenous organic P-cycling-related gene abundance is rarely reported.…”

Section: Relationship Between Pb Immobilization and P Solubilizationsupporting

confidence: 90%

“…Primer sequences for the target genes were applied for qPCR based on previous literatures. Primer bppF (5 -GAC GCA GCC GAY GAY CCN GCN NTN TGG-3 ) and primer bppR (5 -CAG GSC GCA NRT CAN CRT TRT T-3 ) were employed to amplify bpp gene (Huang et al, 2009); primer ALPS-F730 (5 -CAG TGG GAC GAC CAC GAG GT-3 ) and primer ALPS-1101 (5 -GAG GCC GAT CGG CAT GTC G-3 ) were applied to amplify phoD gene (Hu et al, 2018); primer gcdF (5 -CGG CGT CAT CCG GGS NTN YRA YRT-3 ) and primer gcdR (5 -GGG CAT GTC CAT GTC CCA NAD RTC RTG-3 ) were used to amplify gcd gene (Cleton-Jansen et al, 1990). Additionally, primer Eub338 (5 -ACT CCT ACG GGA GGC AGC AG-3 ) and primer Eub518 (5 -ATT ACC GCG GCT GG-3 ) (Fierer et al, 2005) were selected to amplify 16S rRNA gene.…”

Section: Determination Of P-cycling-related Gene Abundancementioning

confidence: 99%

“…Previous literatures have reported that alkaline phosphatase is exclusively originated from soil microorganisms, and acid phosphatase is mainly produced and secreted by plants (Krämer and Green, 2000;Fraser et al, 2015).Three prokaryotic genes, phoX, phoA, and phoD, are responsible for encoding alkaline phosphatase (Huang et al, 2009); three genes of bpp encoding β-propeller phytase, ptp encoding protein tyrosine phosphatase, and hap encoding histidine acid phosphatase are found in prokaryote (Lim et al, 2007;Neal et al, 2017). However, in terrestrial ecosystems, phoD-harboring bacteria are more widely distributed than phoX-, phoA-harboring bacteria (Neal et al, 2017;Hu et al, 2018), and bpp-harboring bacteria are more frequently found than ptp-, hap-harboring bacteria (Lim et al, 2007;Neal et al, 2017). In addition, the hydrolysis of inorganic insoluble P requires the participation of small molecular organic acid (e.g., gluconic acid, lactic acid, and citric acid), which can be released by gcd-harboring bacteria (Vyas and Gulati, 2009;Hanif et al, 2015;Rasul et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of Phosphorus Solubilizing Bacteria With Multiple Phosphorus Sources Utilizing Capability and Their Potential for Lead Immobilization in Soil

Wan

Yin

et al. 2020

Front. Microbiol.

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Phosphorus solubilizing bacteria (PSB) can promote the level of plant-absorbable phosphorus (P) in agro-ecosystems. However, little attention has been paid to PSB harboring abilities in utilizing multiple phosphorus sources and their potentials for heavy metal immobilization. In this study, we applied the strategy of stepwise acclimation by using Ca 3 (PO 4) 2 , phytate, FePO 4 , and AlPO 4 as sole P source. We gained 18 PSB possessing abilities of multiple P sources utilization, and these bacteria belonged to eight genera (Acinetobacter, Pseudomonas, Massilia, Bacillus, Arthrobacter, Stenotrophomonas, Ochrobactrum, and Cupriavidus), and clustered to two apparent parts: Gram-positive bacteria and Gram-negative bacteria. The isolate of Acinetobacter pittii gp-1 presented good performance for utilizing Ca 3 (PO 4) 2 , FePO 4 , AlPO 4 , and phytate, with corresponding P solubilizing levels were 250.77, 46.10, 81.99, and 7.91 mg/L PO 4 3−-P, respectively. The PSB A. pittii gp-1 exhibited good performance for solubilizing tricalcium phosphate in soil incubation experiments, with the highest values of water soluble P and available P were 0.80 and 1.64 mg/L, respectively. Additionally, the addition of A. pittii gp-1 could promote the immobilization of lead (Pb), and the highest Pb immobilization efficiency reached 23%. Simultaneously, we found the increases in abundances of both alkaline phosphatase gene (phoD) and β-propeller phytase gene (bpp) in strain gp-1 added soils. Besides, we observed the expression up-regulation of both pyrroloquinoline quinone gene (pqq) and polyphosphate kinases gene (ppk), with the highest relative expression levels of 18.18 and 5.23, respectively. We also found the polyphosphate particles using granule staining. To our knowledge, our findings first suggest that the solubilizing of tricalcium phosphate by phosphorus solubilizing bacterium belonging to Acinetobacter is coupled with the synthesis of polyphosphate. Taken together, A. pittii gp-1 could be a good candidate in improving soil fertility and quality.

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Section: Relationship Between Pb Immobilization and P Solubilizationsupporting

confidence: 90%

Section: Determination Of P-cycling-related Gene Abundancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of Phosphorus Solubilizing Bacteria With Multiple Phosphorus Sources Utilizing Capability and Their Potential for Lead Immobilization in Soil

Wan

Yin

et al. 2020

Front. Microbiol.

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“…Soil pH was determined with a pH meter (Mettler Toledo, China) according to the methods of Ye et al (2018) and Rana et al (2018). P fractions in soil were analyzed by procedures of Tiessen and Hedley (Hedley et al 1982;Hu et al 2018;Kovar and Pierzynski 2009;Tiessen and Moir 1993;Zhang et al 2009). The Po and Pi forms of soil P were determined through a series of extraction of 0.5 g soil with deionized water, 0.5 M NaHCO 3 , 1 M HCl, and 0.5 M NaOH.…”

Section: Soil and Plant Analysismentioning

confidence: 99%

Mo-Inefficient Wheat Response Toward Molybdenum Supply in Terms of Soil Phosphorus Availability

Rana

Sun

Imran

et al. 2020

J Soil Sci Plant Nutr

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Phosphorus (P) deficiency is a major constraint to crop growth due to strong soil P fixation. The effects of molybdenum (Mo) on leaf anatomy and dynamics of rhizosphere P in Mo-inefficient crops have still not been investigated. A field study was conducted to investigate the effects of long-term fertilization on dynamics of rhizosphere P transformations and leaf anatomy in Moinefficient wheat consisting of Mo (+Mo) and without Mo applied (−Mo) treatments. The results revealed that Mo supply increased plant biomass, grain yield, uptake of P and Mo by 34.9%, 14.8%, 98.1% and 654.1% respectively and preserved the leaf cuticle, stomata, chloroplast, and mesophyll tissue cell configuration. Molybdenum application significantly increased the concentration of radially available P fractions [NaHCO 3-Pi (115.5 to 129.8 mg kg −1 and 67.4 to 80.7 mg kg −1) and H 2 O-Pi (14.4-21.9 mg kg −1 and 4.63-6.40 mg kg −1)] in rhizosphere and non-rhizosphere soils, respectively. The acid phosphatase (ACP) activity (19.5 μmol day −1 g −1) was highest during March as compared to alkaline (ALP) and phytase (PHY) enzymes in the rhizosphere soil of +Mo treatment. The highest expression of gene lppC (6.11) was observed in rhizosphere soil as compared to non-rhizosphere soil which indicated that higher gene expressions induced the higher P enzymatic activities. Our findings suggest that Mo fertilizer application increases P availability through induced alteration in dynamics of rhizosphere soil P fractions, higher P and Mo assimilation and phosphatases enzymes activities along with preserving the leaf anatomy and ultrastructure of Mo-inefficient wheat.

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“…The shear strength of the root-soil composite system is negatively related to the diameter of root, that is, the finer the root system, the greater shear strength of rootsoil composite system [38]. When we predicate slope erosion rate, soil shear strength is an important factor to be considered, because soil shear strength is positively correlated with critical runoff shear stress (τ cr ), and it is negatively correlated with soil erodibility factor (K) [39][40]. It can be seen that sheer strength of the root-soil composite system plays an important role in resisting runoff erosion on the slope, so shear characteristics of the root-soil composite system should be considered in the study of the soil erosion mechanism.…”

Section: Study Of Soil Mechanics Mechanismmentioning

confidence: 99%

Effects of Land Use Change of Sloping Farmland on Characteristic of Soil Erosion Resistance in Typical Karst Mountainous Areas of Southwestern China

Li¹,

Wu²,

Zhang³

et al. 2019

Pol. J. Environ. Stud.

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With the implementation of ecological projects such as the Grain-for-Green Project, rocky desertification control and comprehensive control of soil and water loss for sloping farmland, ecological environment of karst mountainous areas in southwest China has been improved significantly, but soil and water loss of sloping farmland is still an important ecological and environmental problem in this region. In this paper we took a typical karst rocky desertification mountainous area, Jinlan Demonstration Area, as the study area located in Qianxi County, Guizhou Province. On the basis of a previous experimental demonstration of grass planting and afforestation on sloping farmland (planted in 2014), the sample plot method was used to obtain soil samples and soil shearing test piece in 2017, and soil shear strength and soil erodibility factor (K) were measured by direct shear method and hydrometer method in order to study the effect of sloping farmland converted to abandoned land, pastureland and agroforestry on soil erosion resistance. Results showed that the soil sheer strength was no obvious change and soil erodibility was no significant difference (P>0.05) in sloping farmland with the level of rocky desertification becoming more serious; soil sheer strength greatly increased after sloping farmland was abandoned, ultimate shear strength τ 300 was increased by 65.5%, and K was significantly different (P<0.05) after 10 years; in the process of sloping farmland converted to pastureland, the soil ultimate shear strength τ 300 was increased by 34.55% and K decreased significantly (P<0.05), which indicated that the effect of grass planting on improving soil erosion resistance of sloping farmland was obvious; in the process of sloping farmland converted to agroforestry, soil erosion resistance was also enhanced, soil shear strength was increased, and K was decreased (it did not reach the significant level, P>0.05). Based on the preliminary results of this paper, we think both grass (tree) planting and land abandonment could improve soil erosion resistance and reduce soil erosion risk, which *

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Effects of long-term fertilization on phoD-harboring bacterial community in Karst soils

Cited by 148 publications

References 50 publications

Isolation and Characterization of Phosphorus Solubilizing Bacteria With Multiple Phosphorus Sources Utilizing Capability and Their Potential for Lead Immobilization in Soil

Isolation and Characterization of Phosphorus Solubilizing Bacteria With Multiple Phosphorus Sources Utilizing Capability and Their Potential for Lead Immobilization in Soil

Mo-Inefficient Wheat Response Toward Molybdenum Supply in Terms of Soil Phosphorus Availability

Effects of Land Use Change of Sloping Farmland on Characteristic of Soil Erosion Resistance in Typical Karst Mountainous Areas of Southwestern China

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