Effects of Long‐Term Denervation on the Rat Thyroarytenoid Muscle

Miyamaru, Satoru; Kumai, Yoshihiko; Ito, Takaaki; Yumoto, Eiji

doi:10.1097/mlg.0b013e31816f693f

Cited by 33 publications

(32 citation statements)

References 17 publications

(42 reference statements)

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“…Only after this period can we establish a rational treatment plan, such as whether to proceed with surgical intervention. We also examined the effects of long-term denervation on the rat TA muscle and its neuromuscular junctions (NMJs) [6]. Although postsynaptic acetylcholine receptors (AChRs) were observed at 58 weeks after denervation, the number of AChRs decreased gradually after 10 weeks following denervation [6].…”

Section: Introductionmentioning

confidence: 99%

Nerve-muscle pedicle implantation facilitates re-innervation of long-term denervated thyroarytenoid muscle in rats

Miyamaru¹,

Kumai²,

Ito

et al. 2009

Acta Oto-Laryngologica

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Conclusions: Nerve-muscle pedicle (NMP) implantation was effective in the recovery from atrophic changes in long-term denervated thyroarytenoid (TA) muscle. Re-innervation occurred via the transferred nerve. However, the effectiveness of the NMP method may decline with increasing duration of denervation. Objectives: To evaluate the effects of NMP implantation on long-term denervated rat TA muscle. Materials and methods: Wistar rats (n 0105) were divided into two groups in which the left recurrent laryngeal nerve (RLN) was transected without (DNV group) or with (NMP group) subsequent NMP implantation, and subgroups of each group were formed depending on the period after RLN transection (immediate to 48 weeks). In the DNV subgroups, we histologically assessed the area of muscle and the number of neuromuscular junctions. In the NMP subgroups, we performed electromyographic, videolaryngoscopic, and histologic assessments. The muscle area and muscle action potentials were evaluated by comparing the treated and untreated sides. The ratio of the number of nerve terminals to that of acetylcholine receptors was also assessed. Results: The TA muscle area was significantly larger in most of the NMP subgroups compared with the DNV subgroups. Muscle action potentials were present in all NMP animals. All histologic and physiologic assessments revealed degradation as the denervation period in the five NMP subgroups.

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Section: Introductionmentioning

confidence: 99%

Nerve-muscle pedicle implantation facilitates re-innervation of long-term denervated thyroarytenoid muscle in rats

Miyamaru¹,

Kumai²,

Ito

et al. 2009

Acta Oto-Laryngologica

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“…21 Although the number of AChRs at the reinnervated neuromuscular junction of transferred muscle flap was studied, 11 this study focused on the correlation between contractile function recovery and changes of AChR and expression of its subunits of transferred muscle flap.…”

Section: Discussionmentioning

confidence: 99%

Correlation of contractile function recovery with acetylcholine receptor changes in a rat muscle flap model

et al. 2010

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The aim of this study was to investigate the correlation between contractile function recovery and changes of acetylcholine receptors (AChR) in a transferred muscle flap following reinnervation. Orthotopic transfer of the gracilis muscle flap with repair of its nerve was performed bilaterally in 48 rats. The rats were randomly divided into six experimental groups based on the time intervals for assessments (1, 4, 5, 10, 20, and 30 weeks). Sixteen gracilis muscle samples from eight rats without surgery were used as the controls. In each group, muscle contractile force and weight were measured (n = 16). The AChR numbers (n = 8) and subunits (epsilon and gamma) mRNA (n = 8) were examined using [(125)I]-alpha-bungarotoxin and fluorescent quantitative-PCR. The results showed the AChR numbers in the muscle flap increased from 4 to 20 weeks after reinnervation and correlated with recovery of the tetanic contraction force. However, correlation between the increase of AChR number with the specific tension (peak contractile force normalized to wet muscle weight) was only found from 4 to 10 weeks postoperatively. The expression of gamma-subunit mRNA increased at the early period after flap transfer and then decreased rapidly, whereas the epsilon-subunit mRNA recovered gradually since fourth week postoperatively. A small amount of gamma-subunit mRNA could still be detected at 30 weeks after surgery. In conclusion, following reinnervation of the transferred muscle flap, the contractile functional recovery is partially correlated to increase of the AChRepsilon. Our findings may provide evidence for further study of improving muscle function in functional reconstruction by targeting the AChR.

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“…The areas were measured in pixel numbers by tracing the outlines of the TA muscle and its individual muscle fibers using NIH Image software (NIH, Bethesda, MD, USA). To evaluate the NMJs, the nerve terminals (NTs) and acetylcholine receptors (AChRs) were detected immunohistochemically, as described previously [8]. We selected sections from a region of the middle one-third of the TA muscle that was rich in NMJs, as reported previously [9].…”

Section: Methodsmentioning

confidence: 99%

Nerve-muscle pedicle implantation in the denervated thyroarytenoid muscle of aged rats

Miyamaru

Kumai

Minoda

et al. 2011

Acta Oto-Laryngologica

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Effects of Long‐Term Denervation on the Rat Thyroarytenoid Muscle

Cited by 33 publications

References 17 publications

Nerve-muscle pedicle implantation facilitates re-innervation of long-term denervated thyroarytenoid muscle in rats

Nerve-muscle pedicle implantation facilitates re-innervation of long-term denervated thyroarytenoid muscle in rats

Correlation of contractile function recovery with acetylcholine receptor changes in a rat muscle flap model

Nerve-muscle pedicle implantation in the denervated thyroarytenoid muscle of aged rats

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