Effects of long-term cryopreservation on peripheral blood progenitor cells

Vosganian, Gregory; Waalen, Jill; Kim, Kevin; Jhatakia, Sejal; Schram, Ethan; Lee, Tracey; Riddell, Dan; Mason, James R.

doi:10.3109/14653249.2012.706707

Cited by 15 publications

(5 citation statements)

References 22 publications

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“…Overall, despite conflicting results, there is some consensus in published literature on the effects of long-term cryopreservation of bone marrow cells or PBPCs and suggests a loss of functionality with increase in freezing storage time as well as the storage conditions; specifically, storage in dry ice or higher storage temperatures than in liquid nitrogen do lead to cell degradation and loss of functionality. For example, PBPCs frozen and stored in the vapor phase of liquid nitrogen for 15 years resulted in suboptimal activity and reduced viability of white and red blood cells whereas no significant differences were found in samples stored up to 10 years 29 . Human bone marrow stored for overs ten years in the vapor phase of liquid nitrogen have also been shown to form lower colony forming units in culture (CFU-C) and burst forming unit-erythroid (BFU-E) capacity although higher viable cells were found post thawing 50 .…”

Section: Discusssionmentioning

confidence: 99%

“…A study conducted on the peripheral blood progenitor cells stored for longer than 10 years reported the decrease in the viability and activity of red cell colonies and white cell colonies 29 . Likewise, prior studies have reported that the osteogenic potential of cryopreserved ASCs was found to be impeded both in vitro and in vivo in comparison to fresh ASCs 30 .…”

Section: Introductionmentioning

confidence: 99%

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Effects of Decade Long Freezing Storage on Adipose Derived Stem Cells Functionality

Shaik

Gimble

et al. 2018

Sci Rep

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Over the last decade and half, the optimization of cryopreservation for adipose tissue derived stromal/stem cells (ASCs) especially in determining the optimal combination of cryoprotectant type, cooling rate, and thawing rate have been extensively studied. In this study, we examined the functionality of ASCs that have been frozen-stored for more than 10 years denoted as long-term freezing, frozen within the last 3 to 7 years denoted as short-term freezing and compared their response with fresh ASCs. The mean post-thaw viability for long-term frozen group was 78% whereas for short-term frozen group 79% with no significant differences between the two groups. The flow cytometry evaluation of stromal surface markers, CD29, CD90, CD105, CD44, and CD73 indicated the expression (above 95%) in passages P1-P4 in all of the frozen-thawed ASC groups and fresh ASCs whereas the hematopoietic markers CD31, CD34, CD45, and CD146 were expressed extremely low (below 2%) within both the frozen-thawed and fresh cell groups. Quantitative real time polymerase chain reaction (qPCR) analysis revealed some differences between the osteogenic gene expression of long-term frozen group in comparison to fresh ASCs. Intriguingly, one group of cells from the short-term frozen group exhibited remarkably higher expression of osteogenic genes in comparison to fresh ASCs. The adipogenic differentiation potential remained virtually unchanged between all of the frozen-thawed groups and the fresh ASCs. Long-term cryopreservation of ASCs, in general, has a somewhat negative impact on the osteogenic potential of ASCs, especially as it relates to the decrease in osteopontin gene expression but not significantly so with respect to RUNX2 and osteonectin gene expressions. However, the adipogenic potential, post thaw viability, and immunophenotype characteristics remain relatively intact between all the groups.

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Section: Discusssionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Decade Long Freezing Storage on Adipose Derived Stem Cells Functionality

Shaik

Gimble

et al. 2018

Sci Rep

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“…Vosganian et al also did not reveal any decrease in clonogenic activity of PBSCs following 10 years of cryopreservation. However, in samples stored over 10 years, activity decreased significantly 40 . Published data confirms the functional progenitor cell fraction within PBSC grafts is retained for at least 14 years.…”

Section: Discussionmentioning

confidence: 93%

Long-Term Cryopreservation Does Not Affect Quality of Peripheral Blood Stem Cell Grafts: A Comparative Study of Native, Short-Term and Long-Term Cryopreserved Haematopoietic Stem Cells

Lysák¹,

Brychtová

Leba

et al. 2021

Cell Transplant

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Cryopreserved haematopoietic progenitor cells are used to restore autologous haematopoiesis after high dose chemotherapy. Although the cells are routinely stored for a long period, concerns remain about the maximum storage time and the possible negative effect of storage on their potency. We evaluated the effect of cryopreservation on the quality of peripheral stem cell grafts stored for a short (3 months) and a long (10 years) period and we compared it to native products.The viability of CD34+ cells remained unaffected during storage, the apoptotic cells were represented up to 10% and did not differ between groups. The clonogenic activity measured by ATP production has decreased with the length of storage (ATP/cell 1.28 nM in native vs. 0.63 in long term stored products, P < 0.05). Only borderline changes without statistical significance were detected when examining mitochondrial and aldehyde dehydrogenase metabolic activity and intracellular pH, showing their good preservation during cell storage. Our experience demonstrates that cryostorage has no major negative effect on stem cell quality and potency, and therefore autologous stem cells can be stored safely for an extended period of at least 10 years. On the other hand, long term storage for 10 years and longer may lead to mild reduction of clonogenic capacity. When a sufficient dose of stem cells is infused, these changes will not have a clinical impact. However, in products stored beyond 10 years, especially when a low number of CD34+ cells is available, the quality of stem cell graft should be verified before infusion using the appropriate potency assays.

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“…[16][17][18] Cryopreservation is based on the principle that chemical, biological, Activation of MSC's into final cell therapy product; (11) Shipping of final product in optimized, approved delivery device to medical center at 4 °C in temperature controlled packaging with temperature recorder; (12) injection of the cell therapy product into patient. notes: *In the event that a cGmP compliant cell therapy processing unit exists within the medical center, frozen mSCs could theoretically be shipped to the medical center for thawing and further processing at that unit before delivery to the patient.…”

Section: Cryopreservation Considerationsmentioning

confidence: 99%

Cryopreservation and Cell Banking for Autologous Mesenchymal Stem Cell-Based Therapies

Harel¹

2013

CTTT

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As cell-based therapies begin to progress through Phase III clinical trials, there is an increasing need for the development of comprehensive cell banking strategies. In order to achieve commercial viability, both autologous and allogeneic approaches must have a comprehensive, end-to-end cell banking model-including proper collection, manufacturing and release criteria, cryopreservation and storage of cells, shipping, delivery, and logistics management of the final cell product. By developing an understanding of industry standards and best practices across these areas, companies can be better positioned to reduce research costs, improve efficiencies, create revenue streams, decrease time to discovery and, ideally, increase the likelihood and number of approved marketed products. The focus of this paper will be on cell banking strategies for autologous-based cell therapies with mesenchymal stromal cells, which are the most widely used cell type in cell therapy clinical trials today.

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Effects of long-term cryopreservation on peripheral blood progenitor cells

Cited by 15 publications

References 22 publications

Effects of Decade Long Freezing Storage on Adipose Derived Stem Cells Functionality

Effects of Decade Long Freezing Storage on Adipose Derived Stem Cells Functionality

Long-Term Cryopreservation Does Not Affect Quality of Peripheral Blood Stem Cell Grafts: A Comparative Study of Native, Short-Term and Long-Term Cryopreserved Haematopoietic Stem Cells

Cryopreservation and Cell Banking for Autologous Mesenchymal Stem Cell-Based Therapies

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