Effects of long duration, low dose bronopol exposure on the control of Ichthyophthirius multifiliis (Ciliophora), parasitising rainbow trout (Oncorhynchus mykiss Walbaum)

Picón-Camacho, Sara M.; Taylor, Nicholas G H; Bron, James E.; Guo, Fu Ci; Shinn, Andrew P.

doi:10.1016/j.vetpar.2011.11.022

Cited by 22 publications

(16 citation statements)

References 30 publications

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“…Low concentrations of pentagalloylglucose at 1 or 0.5 mg/L could not prevent infestation of theronts (100% prevalence), but significantly reduced infectivity intensity. Long exposure duration to low doses of bronopol has also resulted in the reduction of trophont density in rainbow trout (Picón-Camacho et al, 2012a).…”

Section: Catfish Number/tankmentioning

confidence: 99%

Evaluation of an antiparasitic compound extracted from Galla chinensis against fish parasite Ichthyophthirius multifiliis

Zhang

Klesius

2013

Veterinary Parasitology

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Section: Catfish Number/tankmentioning

confidence: 99%

Evaluation of an antiparasitic compound extracted from Galla chinensis against fish parasite Ichthyophthirius multifiliis

Zhang

Klesius

2013

Veterinary Parasitology

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“… e Activity was measured at 25 °C, 100 mM Tris-HCl buffer pH 8.0, 100 mM KCl, 3 mM EDTA. hIMPDH II: 400 μM NAD + , 200 μM IMP 19 . …”

Section: Resultsmentioning

confidence: 99%

Repurposing existing drugs: identification of irreversible IMPDH inhibitors by high-throughput screening

Sarwono

Mitsuhashi

Kabir

et al. 2018

Journal of Enzyme Inhibition and Medicinal Chemistry

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Inosine 5′-monophosphate dehydrogenase (IMPDH) is an essential enzyme for the production of guanine nucleotides. Disruption of IMPDH activity has been explored as a therapeutic strategy for numerous purposes, such as for anticancer, immunosuppression, antiviral, and antimicrobial therapy. In the present study, we established a luciferase-based high-throughput screening system to identify IMPDH inhibitors from our chemical library of known bioactive small molecules. The screening of 1400 compounds resulted in the discovery of three irreversible inhibitors: disulfiram, bronopol, and ebselen. Each compound has a distinct chemical moiety that differs from other reported IMPDH inhibitors. Further evaluation revealed that these compounds are potent inhibitors of IMPDHs with kon values of 0.7 × 104 to 9.3 × 104 M−1·s−1. Both disulfiram and bronopol exerted similar degree of inhibition to protozoan and mammalian IMPDHs. Ebselen showed an intriguing difference in mode of inhibition for different IMPDHs, with reversible and irreversible inhibition to each Cryptosporidium parvum IMPDH and human IMPDH type II, respectively. In the preliminary efficacy experiment against cryptosporidiosis in severe combined immunodeficiency (SCID) mouse, a decrease in the number of oocyst shed was observed upon the oral administration of disulfiram and bronopol, providing an early clinical proof-of-concept for further utilization of these compounds as IMPDH inhibitors.

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“…Further, this agent has antiprotozoal activity against the whitespot‐disease–causing protista Ichthyophthirius multifiliis in rainbow trout (Picón‐Camacho et al . ; Shinn et al . ).…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo efficacy of drugs against the protozoan parasite Azumiobodo hoyamushi that causes soft tunic syndrome in the edible ascidian Halocynthia roretzi (Drasche)

Park

Zeon

et al. 2013

Journal of Fish Diseases

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It was discovered recently that infection by a protozoan parasite, Azumiobodo hoyamushi, is the most probable cause for soft tunic syndrome in an edible ascidian, Halocynthia roretzi (Drasche). In an attempt to develop measures to eradicate the causative parasite, various drugs were tested for efficacy in vitro and in vivo. Of the 20 antiprotozoal drugs having different action mechanisms, five were found potent (24-h EC50 < 10 mg L(-1) ) in their parasite-killing effects: formalin, H2 O2 , bithionol, ClO2 and bronopol. Moderately potent drugs (10 < 24-h EC50 < 100 mg L(-1) ) were quinine, fumagillin, amphotericin B, ketoconazole, povidone-iodine, chloramine-T and benzalkonium chloride. Seven compounds, metronidazole, albendazole, paromomycin, nalidixic acid, sulfamonomethoxine, KMnO4 , potassium monopersulphate and citric acid, exhibited EC50 > 100 mg L(-1) . When ascidians were artificially infected with A. hoyamushi, treated using 40 mg L(-1) formalin, bronopol, ClO2 , or H2 O2 for 1 h and then monitored for 24 h, very low mortality was observed. However, the number of surviving parasite cells in the ascidian tunic tissues was significantly reduced by treating with 40 mg L(-1) formalin or ClO2 for 1 h. The data suggest that we might be able to develop a disinfection measure using a treatment regimen involving commonly available drugs.

show abstract

Effects of long duration, low dose bronopol exposure on the control of Ichthyophthirius multifiliis (Ciliophora), parasitising rainbow trout (Oncorhynchus mykiss Walbaum)

Cited by 22 publications

References 30 publications

Evaluation of an antiparasitic compound extracted from Galla chinensis against fish parasite Ichthyophthirius multifiliis

Evaluation of an antiparasitic compound extracted from Galla chinensis against fish parasite Ichthyophthirius multifiliis

Repurposing existing drugs: identification of irreversible IMPDH inhibitors by high-throughput screening

In vitro and in vivo efficacy of drugs against the protozoan parasite Azumiobodo hoyamushi that causes soft tunic syndrome in the edible ascidian Halocynthia roretzi (Drasche)

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