Effects of Long-Chain Fatty Acids on Cytosolic Triacylglycerol Accumulation and Lipid Droplet Formation in Primary Cultured Bovine Mammary Epithelial Cells

Yonezawa, Tomo; Yonekura, Shinichi; Kobayashi, Yasuhiko; Hagino, Akihiko; Katoh, K.; Obara, Yoshiaki

doi:10.3168/jds.s0022-0302(04)73377-9

Cited by 47 publications

(65 citation statements)

References 21 publications

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“…In contrast to SCFA, MCFA and LCFA either saturated or unsaturated inhibited leptin expression in bovine adipocytes, in line with those reported in rat and mouse adipocytes [21][22][23][24][25], although they increase leptin expression in bovine mammary epithelial cells [27,28]. The mechanism of the inhibitory effect of LCFA in bovine adipocytes is still obscure, but one plausible explanation is that LCFA acts as a PPAR-γ agonist that has been shown to inhibit leptin expression and secretion in adipocytes [43].…”

Section: Discussionsupporting

confidence: 83%

“…Similarly, acetate and butyrate stimulate leptin expression in bovine anterior pituitary cells [26]. However, MCFA and LCFA such as caprylate and linoleate, respectively, increase leptin expression in bovine mammary epithelial cells [27,28]. In addition, acetate and butyrate fail to influence leptin expression in bovine mammary epithelial cells [28].…”

Section: Introductionmentioning

confidence: 99%

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Inverse regulation of leptin mRNA expression by short- and long-chain fatty acids in cultured bovine adipocytes

Soliman

Kimura

Ahmed

et al. 2007

Domestic Animal Endocrinology

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Leptin is an adipose tissue-derived cytokine plays key roles in the regulation of food intake and energy expenditure. However, regulatory mechanisms of leptin gene expression are not fully elucidated in ruminants that utilize short-chain fatty acids (SCFA), known as volatile fatty acids, as principal energy sources. In this study, we determined effects of SCFA and long-chain fatty acids (LCFA) on leptin expression in bovine adipocytes. Bovine stromal vascular cells isolated from subcutaneous adipose tissue of Holstein cows were cultured to confluence and treated sequentially with dexamethasone and isobutylmethylxanthine for 2 days and insulin and troglitazone for 12 days to achieve full differentiation to adipocytes. The cells started to accumulate lipids 4 days after the onset of treatment, with increased mRNAs expression of leptin, as well as aP2, adiponectin, and PPAR-γ  Removal of fetal calf serum and reduction of glucose in the culture medium of differentiated adipocytes decreased leptin mRNA expression.Subsequent addition of acetate, butyrate, or propionate dose-dependently restored and rather increased leptin expression, while addition of LCFA suppressed it. The stimulatory effect of acetate was abolished by prior treatment of the cells with pertussis toxin and by addition of LCFA. Furthermore, cows fasted for 48h and fed thereafter, elaborate reduced and increased plasma leptin levels, respectively. Thus, these results suggest that plasma leptin levels in cows are inversely controlled at transcription by VFA and LCFA, and that the effects of SCFA possibly act through a G protein-coupled receptor for SCFA.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Inverse regulation of leptin mRNA expression by short- and long-chain fatty acids in cultured bovine adipocytes

Soliman

Kimura

Ahmed

et al. 2007

Domestic Animal Endocrinology

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“…Elsewhere, bovine PPAR1 and PPAR2 cDNAs have been characterized (Sundvold et al, 1997) with expression of the two isoforms in adipose tissue, whereas only PPARg2 was expressed in the mammary gland. Recently, in primary cultured bovine MEC, the expression of PPARg2 in response to the addition of acetate and octanoate was increased while ACC activity decreased (Yonezawa et al, 2004a), conversely to previous observations in adipose tissue (Lee & Hossner, 2002). Elsewhere, an upregulation of mammary PPAR gene expression was shown in dairy cows between -14 and þ14 days relative to parturition, using a bovine cDNA microarray (Loor et al, 2004c).…”

Section: Pparsmentioning

confidence: 77%

Expression and Nutritional Regulation of Lipogenic Genes in the Ruminant Lactating Mammary Gland

Bernard

Leroux

Chilliard

Advances in Experimental Medicine and Biology

206

225

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The effect of nutrition on milk fat yield and composition has largely been investigated in cows and goats, with some differences for fatty acid (FA) composition responses and marked species differences in milk fat yield response. Recently, the characterization of lipogenic genes in ruminant species allowed in vivo studies focused on the effect of nutrition on mammary expression of these genes, in cows (mainly fed milk fat-depressing diets) and goats (fed lipid-supplemented diets). These few studies demonstrated some similarities in the regulation of gene expression between the two species, although the responses were not always in agreement with milk FA secretion responses. A central role for trans-10 C18:1 and trans-10, cis-12 CLA as regulators of milk fat synthesis has been proposed. However, trans-10 C18:1 does not directly control milk fat synthesis in cows, despite the fact that it largely responds to dietary factors, with its concentration being negatively correlated with milk fat yield response in cows and, to a lesser extent, in goats. Milk trans-10, cis-12 CLA is often correlated with milk fat depression in cows but not in goats and, when postruminally infused, acts as an inhibitor of the expression of key lipogenic genes in cows. Recent evidence has also proven the inhibitory effect of the trans-9, cis-11 CLA isomer. The molecular mechanisms by which nutrients regulate lipogenic gene expression have yet to be well identified, but a central role for SREBP-1 has been outlined as mediator of FA effects, whereas the roles of PPARs and STAT5 need to be determined. It is expected that the development of in vitro functional systems for lipid synthesis and secretion will allow future progress toward (1) the identification of the inhibitors and activators of fat synthesis, (2) the knowledge of cellular mechanisms, and (3) the understanding of differences between ruminant species.

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“…Se ha encontrado que la adición de ácido oleico ó linoleico en MEC bovinas disminuye la actividad de las enzimas lipogénicas ACC y FAS (24,26). Sin embargo, Yonezawa et al (27) y Enjalbert et al (28) encontraron que la grasa de la leche aumentaba ó no había diferencia. Así mismo, Mahecha et al (1) no encontraron efecto sobre la grasa de la leche al suplementar con lípidos protegidos mientras que Ahnadi et al (29) encontraron reducción.…”

Section: Efecto De Los áCidos Grasos Insaturados Sobre La Cantidad Deunclassified

“…Estudios realizados por Feuermann et al (35) y Yonezawa et al (27), han encontrado asociación entre la adición in vitro de AGI, el cambio en la expresión de mRNA de prolactina y leptina, y la síntesis de triglicéridos. Sin embargo, estos estudios no evaluaron la vía por la cual los AG actuaron para que la leptina ó ellos directamente, incidieran en la síntesis lipídica, tampoco se evaluó el efecto sobre la composición de los triglicéridos.…”

Section: Efecto De Los áCidos Grasos Insaturados Sobre La Cantidad Deunclassified

Síntesis, composición y modificación de la grasa de la leche bovina: Un nutriente valioso para la salud humana

A¹,

L²,

A³

2009

Rev MVZ Córdoba

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RESUMENSe presenta una revisión sobre la composición, estructura y mecanismo de síntesis de la grasa de la leche en la glándula mamaria bovina y del efecto de la suplementación con ácidos grasos insaturados (AGI) sobre la cantidad y calidad de la grasa. Se resalta la importancia de la suplementación con aceites vegetales más aceite de pescado; se sugiere no generalizar el efecto de AGI como negativo sobre la cantidad de grasa de la leche; se muestra la necesidad de relacionar diferentes AGI con la vía regulatoria SREBP y con diferentes enzimas lipogénicas; y se incentiva la investigación del efecto de AGI sobre la composición de ácidos grasos de la leche y su distribución posicional dentro de la estructura lipídica.Palabras clave: Ácidos grasos, glándula mamaria, lípidos, vacas. ABSTRACTIt presents a review about the composition, structure and mechanism of milk fat synthesis in the bovine mammary gland, and about the effect of unsaturated fatty acid (UFA) supplementation on the amount and quality of milk fat. It is enhanced the importance of the supplementation with vegetable plus fish oils; it is suggested that the effect of UFA should not be remarked as negative on the amount of milk fat; It is showed the necessity of relating different UFA with the SREBP´s regulatory way, and with different lipid enzymes; and it is promoted research about the effect of UFA on the milk fatty acid composition and their positional distribution into the lipid structure.

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Effects of Long-Chain Fatty Acids on Cytosolic Triacylglycerol Accumulation and Lipid Droplet Formation in Primary Cultured Bovine Mammary Epithelial Cells

Cited by 47 publications

References 21 publications

Inverse regulation of leptin mRNA expression by short- and long-chain fatty acids in cultured bovine adipocytes

Inverse regulation of leptin mRNA expression by short- and long-chain fatty acids in cultured bovine adipocytes

Expression and Nutritional Regulation of Lipogenic Genes in the Ruminant Lactating Mammary Gland

Síntesis, composición y modificación de la grasa de la leche bovina: Un nutriente valioso para la salud humana

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