Effects of long-acting erythropoietin analog darbepoetin-α on adriamycin-induced chronic nephropathy

Hussein, Abdelaziz M.; El-Dosoky, Mohamed; Handhle, Ahmed; Elserougy, Hanaa G.; Sarhan, Moustafa; Sobh, Mohamed; Hussiny, Mahmoud El; Nashar, Eman Mohamad El

doi:10.1007/s11255-015-1171-1

Cited by 5 publications

(2 citation statements)

References 36 publications

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“…Adriamycin, a highly cytotoxic anti-tumor drug, affects the structure of podocytes by inducing inflammation and apoptosis. [40,41] The primary administration method for creating an ADN model is intravenous injection that can be performed through the caudal vein, [42] penile vein, [43] or femoral vein. [44] In this study, we established a glomerulonephritis model by administering adriamycin via the tail vein.…”

Section: Hederagenin Improved Podocyte Injurymentioning

confidence: 99%

Hederagenin Improves Adriamycin-induced Nephropathy by Inhibiting the JAK/STAT Signaling Pathway

Zhong,

Jia,

Tan

et al. 2024

Integr Med Nephrol Androl

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Background: Glomerulonephritis, a common kidney disease and major cause of end-stage renal disease, lacks effective treatment options. Hederagenin (HDG) exerts potent anti-inflammatory and protective effects on the kidneys and exhibits promise for the treatment of glomerulonephritis. This study aimed to investigate the therapeutic effects and mechanism of action of hederagenin in the context of adriamycin-induced nephropathy (ADN). Methods: C57BL/6 mice were randomly divided into 5 groups that included the control, model, low-dose HDG (20 mg/kg), high-dose HDG (40 mg/kg), and positive control (10 mg/kg irbesartan) groups. ADN was established in mice by administering a single injection of 10 mg/kg adriamycin. Renal pathology and fibrosis were assessed using haematoxylin and eosin (H & E) and Masson’s trichrome staining, whereas in vitro studies were conducted using cultured mouse podocytes (MPC5). Immunofluorescence staining and western blotting were performed to detect inflammation and the protein levels of signaling pathways. Results: The results revealed that HDG significantly improved adriamycin-induced abnormal serum creatinine, albumin, and urea nitrogen levels. HDG treatment reduced glomerular injury and fibrosis, particularly at high doses. Additionally, HDG effectively reduced adriamycin-induced activation of Janus kinase-signal transducer and activator of transcription (JAK/STAT) signaling and renal fibrosis while suppressing CD4+/CD8+ cell ratios in the kidneys and enhancing the immune response. Interestingly, when the JAK/STAT signaling pathway was activated by an agonist, the ameliorative effects of HDG on ADN were inhibited, thus suggesting that JAK/STAT signaling is a key target of HDG. Conclusion: HDG may represent a promising treatment option for glomerulonephritis by inhibiting JAK/STAT-mediated immune-inflammatory responses.

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Section: Hederagenin Improved Podocyte Injurymentioning

confidence: 99%

Hederagenin Improves Adriamycin-induced Nephropathy by Inhibiting the JAK/STAT Signaling Pathway

Zhong,

Jia,

Tan

et al. 2024

Integr Med Nephrol Androl

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“…The activity of serum catalase was assayed using a UV recording spectrophotometer to measure the decrease in absorbance at 240 nm caused by H2O2 breakdown, in accordance with the Beers and Sizer method described by Usoh et al [20].The reduced glutathione (GSH) was determined similar to Hussein et al [21] while malondialdehyde (MDA) was determined using the method of Buege & Aust [22] described in Vo et al [23].…”

Section: Determination Of Biochemical Markers Of Oxidative Stressmentioning

confidence: 99%

Sub-acute studies on the effects of trona consumption at varied low doses on male fertility and antioxidant properties using Wistar Rats

Chima Ernest Orji,

Ugochukwu Kingsley Ezenyimulu,

Mmachukwu Beverly Ifeadike

et al. 2024

World J. Bio. Pharm. Health Sci.

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Geologically known as trona, evaporite, or locally known as greyish potash, akanwu, or kaun, it serves a multitude of purposes in Nigeria. These include, but are not limited to, softening legumes and flesh, augmenting the therapeutic attributes of herbal expectorant remedies, and reducing male libido or fertility. The unrestricted consumption and accessibility of this salt in the local markets of Anambra State, Nigeria, are due to its affordability and accessibility. Currently, there is no investigation into the comprehensive impact of potash's antioxidant properties at various low doses on male fertility. This led to a study that lasted for 28 days and involved rats weighing between 120 g and 140 g. The rats were separated into four groups, with each group consisting of seven rats. Group A was the control group, while groups B, C, and D were the test groups administered doses of 150 mg/kg, 300 mg/kg, and 450 mg/kg of potash, respectively. At the end of the study, the antioxidant properties of potash were tested. Prostate, gland, testis, and blood were collected and analyzed. Data was analyzed with the statistical social science (SPSS) version 27. Results were presented as mean ± standard deviation (SD). A one-way analysis of variance (ANOVA) was used to compare the mean values. Our study found a significant (p<0.05) dose dependent decrease in fertility hormones and semen parameters. Prostate specific antigen (PSA) increased dose-dependently compared to the control. Our findings indicate that trona is rich in antioxidants.

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Effect of Cerium Oxide Nanoparticles on Adriamycin-Induced Nephropathy: Possible Role for Nrf2/HO-1 and TGF-β/Sirt-1 Pathways

Elsaid,

Khalil,

Eid

et al. 2024

BioNanoSci.

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Effects of long-acting erythropoietin analog darbepoetin-α on adriamycin-induced chronic nephropathy

Abstract: Administration of DPO-α alleviates ADR nephropathy and this might due to improvement of Hb content, hyperlipidemia, enhancement of endogenous antioxidants, reduction of apoptosis and tubulointerstitial injury and maintaining the integrity of glomerular membrane.

Cited by 5 publications

References 36 publications

Hederagenin Improves Adriamycin-induced Nephropathy by Inhibiting the JAK/STAT Signaling Pathway

Hederagenin Improves Adriamycin-induced Nephropathy by Inhibiting the JAK/STAT Signaling Pathway

Sub-acute studies on the effects of trona consumption at varied low doses on male fertility and antioxidant properties using Wistar Rats

Effect of Cerium Oxide Nanoparticles on Adriamycin-Induced Nephropathy: Possible Role for Nrf2/HO-1 and TGF-β/Sirt-1 Pathways

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