Effects of liquid storage on amidase activity, DNA fragmentation and motility of turkey spermatozoa

Kotłowska, M.; Dietrich, Mariola A.; Wojtczak, Mariola; Karol, Halina; Ciereszko, Andrzej

doi:10.1016/j.theriogenology.2006.07.013

Cited by 42 publications

(17 citation statements)

References 42 publications

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“…The difference between our results and those of Kotłowska et al (2007) after 150 minutes of in vitro incubation could be caused by the diluent. They used commercial diluent Ovodyl (IMV, I'Aigle, France) compared to our dilution with physiological solution Figure 2.…”

Section: Effect Of Different Temperatures On Spermatozoa Motilitycontrasting

confidence: 97%

“…Samples stored at 15°C and 25°C had motility from 40% to 8%, and those kept at 35°C for 18 hours were immotile. Kotłowska et al (2007) reported that the percentages of turkey spermatozoa motility during 150 minutes and 24 hours storage in the cold (4-7°C) were similar (about 70%). The significant decrease (P < 0.05) was detected after 48 hours of incubation.…”

Section: Effect Of Different Temperatures On Spermatozoa Motilitymentioning

confidence: 95%

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The effect of diluent, temperature and age on turkey spermatozoa motilityin vitro

Slanina

Petrovičová

Miškeje

et al. 2014

Journal of Applied Animal Research

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The aim of this study was to evaluate the turkey spermatozoa motility in in vitro conditions and to prove the effect of different conditions of incubation -diluents, temperature and age of birds. Spermatozoa were obtained from adult turkey's line of Big 6, and spermatozoa motility parameters were evaluated using a computer-assisted semen analyzer (CASA) system. Significant decrease of spermatozoa motility at laboratory temperature (22°C) was detected from time 0 (94.15%) till 180 minutes of incubation (53.91%). At the cool media incubation (5°C), this difference was lower (95.41 and 78.86%, respectively), and the differences were significant from 30 minutes of incubation till 180 minutes. Progressive spermatozoa motility replicated the tendency of total spermatozoa motility. When the physiological solution to commercial diluent at 5°C was compared, the spermatozoa motility and progressive motility in both groups were very consistent for 90 minutes of incubation. Subsequently, significantly higher spermatozoa motility was detected at time periods 120, 150 and 180 minutes of incubation in commercial diluent. Motility was also higher in this group after 24 hours. Influence of age on spermatozoa motility parameters was analysed at 22°C at the time 0 and after 30 minutes of incubation. Analysis of spermatozoa motility as well as progressive spermatozoa motility proved higher values in Group A (aged 35-42 weeks) compared to Group B (aged 63-73 weeks). These results clearly suggest that low temperature and commercial diluents maintain motility parameters during longer time periods and the increasing age of birds has negative impact on motility parameters.

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Section: Effect Of Different Temperatures On Spermatozoa Motilitycontrasting

confidence: 97%

Section: Effect Of Different Temperatures On Spermatozoa Motilitymentioning

confidence: 95%

The effect of diluent, temperature and age on turkey spermatozoa motilityin vitro

Slanina

Petrovičová

Miškeje

et al. 2014

Journal of Applied Animal Research

View full text Add to dashboard Cite

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“…Within 2 h after collection, sperm motility was tested and samples were either subjected to liquid storage or cryopreservation. Samples of extended semen were stored for 48 h under refrigeration (5°C) according to the method described by Kotłowska et al (2007). From each sample, aliquots were removed at 2, 24, and 48 h of storage for sperm motility to be measured.…”

Section: Methodsmentioning

confidence: 99%

Short-term storage and cryopreservation of black grouse Tetrao tetrix and capercaillie T. urogallus semen

et al. 2010

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Assisted reproductive technologies can be an important part of programs directed for maintenance and protection of genetic variability. The objective of this study was to develop methods for liquid storage and cryopreservation of semen capercaillie and black grouse semen. Our results provide for the first time evidence for successful short-term storage and cryopreservation of capercaillie and black grouse semen using criteria of sperm motility characteristics as quality indices. Sperm motility could be protected up to 48 h liquid storage; however, 24-h storage should be preferable. Cryopreservation secured 40-60% post-thaw motility (as compared with control), both for freshly collected and 24-h-stored semen. In conclusion, a procedure for short-term and cryopreservation of capercaillie and black grouse semen are now available. These assisted reproductive technologies can be implemented into captive breeding programs for these species.

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“…Frankic et al (2006) reported that T-2 toxin and deoxynivalenol (DON) induced DNA fragmentation in chicken spleen leukocytes which was abrograted by dietary nucleotides. Kotłowska et al (2007) have demonstrated increased DNA fragmentation in turkey sperm after 48 h of liquid storage and might be helpful in evaluating the DNA integrity for artificial insemination.…”

Section: Comet Assay In Birdsmentioning

confidence: 99%

Comet assay: a reliable tool for the assessment of DNA damage in different models

2008

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New chemicals are being added each year to the existing burden of toxic substances in the environment. This has led to increased pollution of ecosystems as well as deterioration of the air, water, and soil quality. Excessive agricultural and industrial activities adversely affect biodiversity, threatening the survival of species in a particular habitat as well as posing disease risks to humans. Some of the chemicals, e.g., pesticides and heavy metals, may be genotoxic to the sentinel species and/or to non-target species, causing deleterious effects in somatic or germ cells. Test systems which help in hazard prediction and risk assessment are important to assess the genotoxic potential of chemicals before their release into the environment or commercial use as well as DNA damage in flora and fauna affected by contaminated/polluted habitats. The Comet assay has been widely accepted as a simple, sensitive, and rapid tool for assessing DNA damage and repair in individual eukaryotic as well as some prokaryotic cells, and has increasingly found application in diverse fields ranging from genetic toxicology to human epidemiology. This review is an attempt to comprehensively encase the use of Comet assay in different models from bacteria to man, employing diverse cell types to assess the DNA-damaging potential of chemicals and/or environmental conditions. Sentinel species are the first to be affected by adverse changes in their environment. Determination of DNA damage using the Comet assay in these indicator organisms would thus provide information about the genotoxic potential of their habitat at an early stage. This would allow for intervention strategies to be implemented for prevention or reduction of deleterious health effects in the sentinel species as well as in humans.

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Effects of liquid storage on amidase activity, DNA fragmentation and motility of turkey spermatozoa

Cited by 42 publications

References 42 publications

The effect of diluent, temperature and age on turkey spermatozoa motilityin vitro

The effect of diluent, temperature and age on turkey spermatozoa motilityin vitro

Short-term storage and cryopreservation of black grouse Tetrao tetrix and capercaillie T. urogallus semen

Comet assay: a reliable tool for the assessment of DNA damage in different models

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