Effects of liposomal phophatidylserine on phagocytic uptake of liposomes by macrophage-like HL-60RG cells

Makino, Kimiko; Tabata, Junya; Yoshioka, Tatsuya; Fukuda, Minoru; Ikekita, Masahiko; Ohshima, Hiroyuki; Terada, Hiroshi

doi:10.1016/s0927-7765(03)00006-7

Cited by 8 publications

(4 citation statements)

References 30 publications

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“…Although this type of particles is common to inorganic entities, it becomes unrealistic for the description of many organic and biological entities such as polyelectrolytes. – Soft or fuzzy particles, – which comprise a rigid core and a membrane layer, are another example where assuming a rigid entity is inappropriate. Biological cells such as human erythrocytes, the peripheral zone of which contains a glycoprotein layer, can be simulated by soft particles, and therefore are not suitable to be treated as rigid particles. The electrophoresis of soft particles is usually more complicated than that of the corresponding rigid particles because the membrane layer of the former will influence appreciably both its electric and hydrodynamic nature.…”

Section: Introductionmentioning

confidence: 99%

Electrophoresis of a Charge-Regulated Soft Sphere in a Charged Cylindrical Pore

et al. 2010

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The boundary effect on the electrophoresis of a soft spherical particle, which comprises a rigid core and a porous layer, along the axis of a cylindrical pore is analyzed under the conditions of low surface potential and weak applied electric field. The porous layer of the particle is of charge-regulated nature where the distribution of fixed charge depends on the degree of dissociation/association reaction of the ionizable function groups contained in the porous layer. The pore might be charged, implying that the effect of electroosmotic flow can play a role. The influences of the nature of the porous layer of a particle, the thickness of the double layer, the pH of the bulk liquid, and the relative size of a pore on the electrophoretic behavior of the particle are investigated. Several unexpected and interesting results are obtained. For instance, if the pH value of the bulk liquid or the thickness of the membrane layer takes a medium large value, the electrophoretic mobility of the particle has a local maximum as the thickness of the double layer varies.

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Section: Introductionmentioning

confidence: 99%

Electrophoresis of a Charge-Regulated Soft Sphere in a Charged Cylindrical Pore

et al. 2010

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“…The exposure of the phosphatidylserine moiety on the membrane of apoptotic lymphocytes is important for their removal by endocytosis by macrophage cells through recognition via scavenger receptors on the macrophages [32]. Based on this mechanism, liposomes containing phosphatidylserine are more susceptible to uptake by macrophage-like HL-60RG cells than those containing phosphatidylethanolamine or phosphatidic acid [33].…”

Section: Particle Formulationsmentioning

confidence: 99%

Endocytosis of Particle Formulations by Macrophages and Its Application to Clinical Treatment

Hirota¹,

Terada²

2012

Molecular Regulation of Endocytosis

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“…For comparison, Table summarizes the ZN and λ –1 values for various systems that can be modeled as soft particles. − The ZN value of the PDMAAm-modified liposome was smaller than those of human blood cells and HL-60 RG cells. The surfaces of these cells are covered with saccharide chains including negatively charged sialic acid.…”

Section: Resultsmentioning

confidence: 99%

Liposome-Surface-Initiated ARGET ATRP: Surface Softness Generated by “Grafting from” Polymerization

2019

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Liposomes are self-assembled vesicles of amphiphilic lipid molecules, which have been investigated as models of cells, or tools for drug delivery systems. In these systems, the surface property of the liposomes plays an important role. In this study, we demonstrated a novel polymer modification of liposome surfaces using a controlled radical polymerization, “activators regenerated by electron transfer for atom transfer radical polymerization”, in aqueous media without a deoxygenation step. Dynamic light scattering and 1H NMR measurement indicated the successful modification of the polymer on the liposome surface. The molecular weight of the grafted polymer chain was systematically controlled by changing the monomer concentrations in the “grafting from” polymerization. Moreover, the modification resulted in a notable increase in surface softness as indicated by electrophoretic behavior, which was comparable to the surface of cells. The preparation method and the characterization presented in this study would be a helpful guideline in designing the polymer/liposome hybrid having target surface properties.

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Effects of liposomal phophatidylserine on phagocytic uptake of liposomes by macrophage-like HL-60RG cells

Cited by 8 publications

References 30 publications

Electrophoresis of a Charge-Regulated Soft Sphere in a Charged Cylindrical Pore

Electrophoresis of a Charge-Regulated Soft Sphere in a Charged Cylindrical Pore

Endocytosis of Particle Formulations by Macrophages and Its Application to Clinical Treatment

Liposome-Surface-Initiated ARGET ATRP: Surface Softness Generated by “Grafting from” Polymerization

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