Effects of lipopolysaccharide on blood–brain barrier permeability during pentylenetetrazole-induced epileptic seizures in rats

Arıcan, Nadir; Kaya, Mehmet; Kalayci, Rivaze; Uzun, Hafize; Ahıshalı, Bülent; Bılgıç, Bilge; Elmas, İmdat; Küçük, Mutlu; Gürses, Candan; Uzun, Mustafa

doi:10.1016/j.lfs.2005.12.035

Cited by 29 publications

(18 citation statements)

References 44 publications

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“…Furthermore, we observed a trend toward reduced seizure activity in LPS preconditioned mice, similarly to what other groups have reported (Sayyah et al, 2003, Akarsu et al, 2006, Arican et al, 2006, Dmowska et al, 2010. These data would suggest that LPS activated microglia were providing some protection to the hippocampus, perhaps by controlling the spread of seizure activity.…”

Section: A Functional Role For Microglia In Seizures: the 'Bad' And Tsupporting

confidence: 90%

“…There are a few examples in the literature where preconditioning microglia with LPS modulates seizures including our own, and this will be discussed in more detail in the next section (Sayyah et al, 2003, Akarsu et al, 2006, Arican et al, 2006, Dmowska et al, 2010, Mirrione et al, 2010, Yang et al, 2010a. One mechanism that may contribute to the positive effects attributed to LPS preconditioning is through enhanced neurotrophin release (Nakajima et al, 2001a).…”

Section: Wwwintechopencommentioning

confidence: 95%

“…This is akin to reports that sublethal stress stimuli induce tolerance in ischemia (Marsh et al, 2009). In one study, LPS administration prior to pentylenetetrazole (PTZ)-induced seizures was beneficial by increasing plasma levels of NO and IL-6, which reduced blood brain barrier permeability (Arican et al, 2006). In kindling, LPS was inhibitory but only when it was administered daily for 16 days, as it blunted the acquisition of kindled behavioral seizures (Sayyah et al, 2003).…”

Section: A Functional Role For Microglia In Seizures: the 'Bad' And Tmentioning

confidence: 99%

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A Functional Role for Microglia in Epilepsy

Mirrione¹,

Tsirka²

2011

Clinical and Genetic Aspects of Epilepsy

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Section: A Functional Role For Microglia In Seizures: the 'Bad' And Tsupporting

confidence: 90%

Section: Wwwintechopencommentioning

confidence: 95%

Section: A Functional Role For Microglia In Seizures: the 'Bad' And Tmentioning

confidence: 99%

See 1 more Smart Citation

A Functional Role for Microglia in Epilepsy

Mirrione¹,

Tsirka²

2011

Clinical and Genetic Aspects of Epilepsy

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“…In the light of the recent literature data, it appears that the fundamental role of nitric oxide in inducing neuroprotection by preconditioning is mainly related to induction of vasculoprotective effect [44,45] and reduction of BBB disruption and brain edema [40,46]. It was shown that LPS-preconditioning protects from impairment of endothelial and smooth muscle relaxation normally induced by ischemia/reperfusion injury [45], which results in normalization of cerebral blood flow in the penumbral region (moderate ischemia) lasting out to 24 h after middle cerebral artery occlusion [9,20,44].…”

Section: Discussionmentioning

confidence: 99%

Behavioural and Histological Effects of Preconditioning with Lipopolysaccharide in Epileptic Rats

et al. 2009

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Sublethal stress stimuli such as systemic endotoxin treatment can induce tolerance of the brain to subsequent ischemic stress, which results in a decreased infarct size. Based on this evidence, we hypothesized that lipopolysaccharide (LPS)-induced preconditioning could protect hippocampal neurons in epileptic rats. To test this hypothesis, the anticonvulsant effect of a low dose of LPS against seizures elicited by pilocarpine hydrochloride was measured. Using the pilocarpine model of temporal lobe epilepsy and LPS-preconditioning, we also investigated hippocampal pathology in the rat brain. Based on the behavioural observations conducted, it can be assumed that the preconditioning procedure used may decrease seizure excitability in epileptic rats. However, determination of the seizure excitability threshold needs to be elaborated. Qualitative and quantitative analyses of histological brain sections in the LPS-preconditioned rats showed markedly decreased intensity of neurodegenerative changes in the CA1, CA3 and DG hippocampal fields. The tendency was observed in all the periods of the pilocarpine model of epilepsy. We suggest that preconditioning with LPS may have neuroprotective effects in the CA1, CA3 and DG hippocampal sectors; however, it has no influence on the course of the seizures in rats in the pilocarpine model of epilepsy.

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“…Recent studies have suggested that preconditioning microglia with bacterial lipopolysaccharide (LPS) can be protective in rodent seizure models (Akarsu et al, 2006; Arican et al, 2006; Dmowska et al, 2009; Sayyah et al, 2003b), a concept which has been critically investigated in ischemia (Marsh et al, 2009b; Rosenzweig et al, 2004). The mechanism of protection in ischemia has been shown to involve tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and reprogramming of the cellular response to excitotoxic insults (Marsh et al, 2009a; Saha et al, 2009), but the potential for protection in epilepsy is controversial and less understood.…”

Section: Introductionmentioning

confidence: 99%

Microglial ablation and lipopolysaccharide preconditioning affects pilocarpine-induced seizures in mice

Mirrione

Konomos

Gravanis

et al. 2010

Neurobiology of Disease

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Activated microglia have been associated with neurodegeneration in patients and in animal models of Temporal Lobe Epilepsy (TLE), however their precise functions as neurotoxic or neuroprotective is a topic of significant investigation. To explore this, we examined the effects of pilocarpine induced seizures in transgenic mice where microglia/macrophages were conditionally ablated. We found that unilateral ablation of microglia from the dorsal hippocampus did not alter acute seizure sensitivity. However, when this procedure was coupled with lipopolysaccharide (LPS) preconditioning (1 mg/kg given 24 hours prior to acute seizure), we observed a significant pro-convulsant phenomenon. This effect was associated with lower metabolic activation in the ipsilateral hippocampus during acute seizures, and could be attributed to activity in the mossy fiber pathway. These findings reveal that preconditioning with LPS 24 hours prior to seizure induction may have a protective effect which is abolished by unilateral hippocampal microglia/macrophage ablation.

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Effects of lipopolysaccharide on blood–brain barrier permeability during pentylenetetrazole-induced epileptic seizures in rats

Cited by 29 publications

References 44 publications

A Functional Role for Microglia in Epilepsy

A Functional Role for Microglia in Epilepsy

Behavioural and Histological Effects of Preconditioning with Lipopolysaccharide in Epileptic Rats

Microglial ablation and lipopolysaccharide preconditioning affects pilocarpine-induced seizures in mice

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