Effects of Lipid Extraction on Stable Isotope Ratios in Avian Egg Yolk: Is Arithmetic Correction a Reliable Alternative?

Oppel, Steffen; Federer, Rebekka N.; O’Brien, Diane M.; Powell, Abby N.; Hollmén, Tuula E.

doi:10.1525/auk.2009.09153

Cited by 37 publications

(49 citation statements)

References 31 publications

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“…Chief among these is that such compounds often have well defined metabolic pathways that are well understood and which involve no or well-constrained isotopic discrimination (Evershed et al 2007). The nature of metabolic routing of key dietary components strongly suggests that the current difficulty of high variance related to isotopic discrimination and routing can be overcome by adopting an isotopic compound-specific approach to deciphering animal diets by using stable isotopes (Federer et al 2010). For AAs, it is now clear that some differ little in their δ 15 N values through food webs, whereas others change dramatically.…”

Section: Compound-specific Approachesmentioning

confidence: 99%

From lavage to lipids: estimating diets of seabirds

Karnovsky¹,

Hobson²,

Iverson³

2012

Mar. Ecol. Prog. Ser.

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Section: Compound-specific Approachesmentioning

confidence: 99%

From lavage to lipids: estimating diets of seabirds

Karnovsky¹,

Hobson²,

Iverson³

2012

Mar. Ecol. Prog. Ser.

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“…Due to the yolks' high lipid content, which leads to depleted 13 C (Oppel et al, 2010), yolk samples were divided into two subsamples, one of which was delipidated. We followed the methodology of Ehrich et al (2011) and added a 2:1 chloroform-methanol mixture to the dried, powdered yolk.…”

Section: Stable Isotope Analysesmentioning

confidence: 99%

Extreme intra-clutch egg size dimorphism is not coupled with corresponding differences in antioxidant capacity and stable isotopes between eggs

Poisbleau

Beaulieu

Dehnhard

et al. 2017

Comparative Biochemistry and Physiology Part A: Molecular &

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Oviparous females need to allocate resources optimally to their eggs in order to maximize their fitness. Among these resources, dietary antioxidants, acquired by females and transferred to the eggs during egg formation, can greatly affect the development and survival of the embryo and chick. In crested penguins, incubation starts after the second and last egg is laid and, as opposed to many other bird species, this egg hatches first, thereby enhancing the survival of the chick. Here, we assessed whether antioxidant and isotopic composition could underlie these differences between eggs within clutches of southern rockhopper penguins (Eudyptes chrysocome chrysocome). The second-laid egg had higher total antioxidant capacity than the first-laid egg, although this was not due to higher antioxidant concentration but to its higher mass. This suggests that resources are allocated by females at a constant rate in both eggs within clutches. Accordingly, we found a strong correlation for isotopic compositions between eggs suggesting that resources were allocated similarly to each egg within the clutch. Overall, we found little evidence for a significant role of antioxidant and isotopic compositions to explain differences in terms of embryo/chick development between eggs in crested penguins. However, since our results suggest a constant rate of antioxidant transfer from females to eggs, limiting the mass of the first-laid egg might represent a strategy for females to spare antioxidant defences and preserve self-maintenance.

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“…We realize that lipid extraction is not necessary for δ 15 N measurements of egg homogenates per se, and that the process of lipid extraction can have small but systematic effects on sample δ 15 N values (e.g. Oppel et al, 2010). However, because we were interested in comparing sample δ 15 N values under conditions of identical treatment and only wished to examine changes in δ 15 N through time, within and between colonies, we reasoned that lipid extraction would not influence our conclusions.…”

Section: Stable-nitrogen Isotope Analysismentioning

confidence: 99%