“…(Luo et al, 2010;Mohan et al, 2008), freeze-thawing (Mohammadi et al, 2012), repeated-aeration (Ren et al, 2008), and load-shock (Luo et al, 2010;O-Thong et al, 2009). Many of these pretreatments such as treatment with acid and base (BenYi and JunXin, 2009;Luo et al, 2010), BESA , heat-shock (Kotay and Das et al, 2009), repeated-aeration (Ren et al, 2008), combinations of acid, heat and chemicals , and LCFAs (Saady et al, 2012a(Saady et al, , 2012b(Saady et al, , 2012c were reported to be 'the best' in comparison to other methods. The following sections present a review on the effectiveness and deficiencies of specific culture pretreatments concerning H 2 production.…”