Effects of Light on Trace Elements and Triiodothyronine Levels in Plasma of Mirror Carp (Cyprinus carpio)

Abstract

The changes of copper, zinc, iron, magnesium, manganese, and triiodothyronine as a result of short- and long-term isothermal exposure to light were determined in the plasma of mirror carp (Cyprinus carpio). Two groups of fish were subject to a 3-mo treatment that consisted of (1) 8 h of light followed by 16 h of darkness and (2) 16 h of light followed by 8 h of darkness at 25 degrees C. No significant changes were recorded between the two groups at the beginning of the study. After 3 mo, the triiodothyronine and elements levels as well as the growth rates of fish in the long-time exposure period were significantly higher than those in the short-time exposure (p<0.05).

The synthesis of some β-lactams and investigation of their metal-chelating activity, carbonic anhydrase and acetylcholinesterase inhibition profiles

“…Bradford method was used for the determination of protein quantity during the purification steps 70 . This spectrophotometrical protein assay was explained as previously 71 . The bovine serum albumin was used as standard protein which demonstrated maximum activity at 595 nm [71][72][73] .…”

“…This spectrophotometrical protein assay was explained as previously 71 . The bovine serum albumin was used as standard protein which demonstrated maximum activity at 595 nm [71][72][73] . After the purification process of the CA isoenzymes, SDS-PAGE has been carried out [74][75][76] .…”