Effects of Light of Different Spectral Composition on Refractive Development and Retinal Dopamine in Chicks

Wang, Min; Schaeffel, Frank; Jiang, Bing; Feldkaemper, Marita

doi:10.1167/iovs.18-23880

Cited by 85 publications

(70 citation statements)

References 54 publications

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“…The anterior segment of the eye was discarded. Vitreous and retina were prepared as described before, 27 instantly frozen in liquid nitrogen, and stored at À808C. Catecholamines were quantified with HPLC-electrochemical detection (Ultimate 3000 LC with electrochemical detection ECD3000RS; Thermo Fisher Scientific, Waltham, MA, USA) as previously described.…”

Section: Inhibition Of Myopia With Artificial Stimulimentioning

confidence: 99%

“…Catecholamines were quantified with HPLC-electrochemical detection (Ultimate 3000 LC with electrochemical detection ECD3000RS; Thermo Fisher Scientific, Waltham, MA, USA) as previously described. 27 Vitreous was mixed with 750 lL mobile phase (Thermo Fisher Scientific) and homogenized with a tissue lyser (TissuesLyser LT; Qiagen, Hilden, Germany) at 50 Hz for 4 minutes, while the retina was disrupted with 500 lL mobile phase and 25 lL of the homogenate was saved for later protein measurements. Both the vitreous and retina homogenates were centrifuged at 14,000g for 10 minutes, the supernatant filtered through a 0.2-lm nylon membrane sample filter (Thermo Scientific, Rockwood, IL, USA), and 25 lL was directly injected into the HPLC system.…”

Section: Inhibition Of Myopia With Artificial Stimulimentioning

confidence: 99%

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Probing the Potency of Artificial Dynamic ON or OFF Stimuli to Inhibit Myopia Development

Wang

Aleman

Schaeffel

2019

Invest. Ophthalmol. Vis. Sci.

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Section: Inhibition Of Myopia With Artificial Stimulimentioning

confidence: 99%

Section: Inhibition Of Myopia With Artificial Stimulimentioning

confidence: 99%

Probing the Potency of Artificial Dynamic ON or OFF Stimuli to Inhibit Myopia Development

Wang

Aleman

Schaeffel

2019

Invest. Ophthalmol. Vis. Sci.

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“…All homogenized samples were centrifuged at 4 ° C for 10 min at 14,000 g. The supernatant was filtered through a 0.2-µm nylon membrane filter (Thermo Scientific, Rockwood, MI, USA), and 25 µL was directly injected into the HPLC system. Samples were analyzed for catecholamine and indolamine content by HPLC (Ultimate 3000 LC with electrochemical detection ECD3000RS, Thermo Fischer Scientific) with coulometric detection as previously described [39]. In brief, a hypersil C18 column was used (150 × 3 mm, 3 µm) together with a test mobile phase (Thermo Fischer Scientific) containing 10% acetonitrile and 1% phosphate buffer.…”

Section: Sample Preparation and Measurement Of Biogenic Amines By Hplmentioning

confidence: 99%

“…Recently, it has been demonstrated that also vitreal HVA content DOI: 10.1159/000502529 is a good measure of dopamine turnover, since HVA content in the vitreous was significantly correlated with the amount of vitreal DOPAC [39].…”

Section: Sample Preparation and Measurement Of Biogenic Amines By Hplmentioning

confidence: 99%

Effects of 7-Methylxanthine on Deprivation Myopia and Retinal Dopamine Release in Chickens

Liu

Schaeffel

Trier³

et al. 2019

Ophthalmic Res

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Introduction: Intake of 7-methylxanthine (7-MX), an adenosine receptor (AR) antagonist, has been shown to inhibit school myopia in children and deprivation myopia in rhesus monkeys, but the underlying mechanisms are not known. Also retinal dopamine seems to be involved in the control of eye growth, and in the brain, ARs and dopamine receptors interact widely by heteromerization. We have studied whether 7-MX can inhibit deprivation myopia also in chickens and whether inhibition may involve the retinal dopamine system. Methods: 7-MX was applied by either tubefeeding (100 µg/g body weight, twice a day) or intravitreal injection (12.5 µg, every other day). Forty-eight 2-week-old chicks wore unilateral diffusers and were randomly assigned to either the tube-feeding group (involving 7-MX, vehicle [xanthan gum], or no feeding, for 13 days) or the intravitreal injection group (involving 7-MX, vehicle, or DMSO, for 8 days). Refractions (REs), ocular biometry (AL, VCD), and scleral and choroidal thickness (ChT) were measured before and after treatment. Dopamine and dihydroxyphenylacetic acid (DOPAC) content were determined in retina and vitreous by HPLC at the end of the experiments. Results: No matter how 7-MX was applied, it did not inhibit deprivation myopia in chicks. No significant differences were observed in RE, VCD, AL, and scleral fibrous layer thickness. Feeding 7-MX produced more choroidal thinning in the open contralateral eye compared to control eyes in the vehicle-fed group (-40 ± 14 vs. -1 ± 7 µm, unpaired t test, p < 0.05). DOPAC and dopamine concentration in vitreous and DOPAC concentration in retina did not change with 7-MX. Vitreal dopamine content was significantly decreased in deprived eyes in the groups fed with the vehicle xanthan gum (paired t test, p < 0.01) but not in 7-MX-treated eyes, perhaps indicating a small effect of 7-MX on dopamine. Conclusions: In our study, 7-MX had no effect on DM in chicks and only minor effects on ChT and retinal dopamine. It remains unclear whether 7-MX inhibits myopia through a retinal mechanism or whether it acts directly on choroid and sclera. In the latter case, the finding that myopia is suppressed in mammals but not birds might be explained by differences in scleral structure.

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“…blue light [34]. Besides the anti-myopic effect of blue light attributed to the myopic defocus it causes on the retina (discussed above) [20], it has a further protective effect mediated in part by dopamine through pre-and postsynaptic connections of ipRGCs to dopaminerg amacrin cells [35]. Dopamine has been long acknowledged as a retinal neurotransmitter acting against myopia development, and it has also been evidenced that blue light stimulates a larger amount of dopamine release than other wavelengths do [35].…”

Section: Discussionmentioning

confidence: 99%

Myopia-26, the female-limited form of early-onset high myopia is associated with retinal ganglion cell dysfunction and primary open angle glaucoma

Széll

Fehér

Maróti

et al. 2020

Preprint

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Background: Female-limited early-onset high myopia, also called Myopia-26 is a rare monogenic disorder characterized by severe short sightedness starting in early childhood and progressing to blindness potentially by the middle ages. Despite the X-linked locus of the mutated ARR3 gene, the disease paradoxically affects females only, with males being asymptomatic carriers. Previously, this disease has only been observed in Asian families and has not gone through detailed investigation concerning collateral symptoms or pathogenesis Results: We found a large Hungarian family displaying female-limited early-onset high myopia. Whole exome sequencing of two individuals identified a novel nonsense mutation (c.214C>T, p.Arg72*) in the ARR3 gene. We carried out basic ophthalmological testing for 18 family members, as well as detailed ophthalmological examination (intraocular pressure, axial length, fundus appearance, optical coherence tomography, visual field- and colour vision testing) and electrophysiology tests (standard and multifocal electroretinography, pattern electroretinography and visual evoked potentials) for eight individuals. We found variously impaired visual fields in four affected myopic females and a carrier male. Abnormal pattern electroretinography responses were detected in both symptomatic and carrier patients. Conclusions: This is the first description of a Caucasian family displaying Myopia-26. Pattern electroretinography implies retinal ganglion cell dysfunction present in both sexes. In conjunction with the visual field loss, this suggests the development of age-related open angle glaucoma in addition to high myopia, both attributable to the rare genetic variant of ARR3. We present two hypotheses that could potentially explain the pathomechanism of this disease.

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Effects of Light of Different Spectral Composition on Refractive Development and Retinal Dopamine in Chicks

Cited by 85 publications

References 54 publications

Probing the Potency of Artificial Dynamic ON or OFF Stimuli to Inhibit Myopia Development

Probing the Potency of Artificial Dynamic ON or OFF Stimuli to Inhibit Myopia Development

Effects of 7-Methylxanthine on Deprivation Myopia and Retinal Dopamine Release in Chickens

Myopia-26, the female-limited form of early-onset high myopia is associated with retinal ganglion cell dysfunction and primary open angle glaucoma

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