Effects of light, macronutrients, and sucrose on germination and development of the endangered fern Adiantum reniforme var. sinense (Adiantaceae)

Wu, Hao; Liu, Xiu‐Qun; Ji, Hua; Chen, Long-Qing

doi:10.1016/j.scienta.2010.03.004

Cited by 25 publications

(18 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…sinense Y. X. Lin. (Wu et al 2010). The number of spathulate-and heart-shaped gametophytes was greatest on this medium, whereas gametophyte development was inhibited on full-and halfstrength MS medium.…”

Section: Mass Multiplication Of Fern Gametophytesmentioning

confidence: 83%

“…f. showed excellent sporophyte-producing capacity when gametophytes were cultured on plain agar gel (Fernández et al 1999). Wu et al (2010) showed that the rate of sporophyte production in A. reniforme var. sinense cultured in pure river sand was greater than that in a mixture of soil and sand.…”

Section: Control Of Sexual Reproduction In Fernsmentioning

confidence: 99%

“…Application of these technologies is still restricted to a limited number of rare and threatened species (Pence 2014;Kaczmarczyk et al 2011). Similar research into both in vitro and liquid nitrogen (LN) techniques for the conservation of ferns is described in only few reports (Wu et al 2010;Barnicoat et al 2011;Marszał-Jagacka and Kromer 2011;Mikuła et al 2011;Rybczyński and Mikuła 2011;Ibars and Estrelles 2012). The Red List of Threatened Species (IUCN 2014) clearly shows that more than half of fern species and their allies are threatened to some degree.…”

Section: Introductionmentioning

confidence: 99%

“…Tissue culture provides optimal nutritional and environmental conditions for large-scale fern production, often in the absence of exogenous plant growth regulators (PGRs), even for inducing somatic embryogenesis (Mikuła et al 2015b). During the in vitro culture of ferns, many factors affecting the efficiency of multiplication of both gametophyte (Fernández et al 1997;Sheffield et al 1997;Goller and Rybczyński 2007;Wu et al 2010;Moura et al 2012) and sporophyte (Hirsch 1975;Materi and Cumming 1991;Teng 1997;Ambrósio and de Melo 2004;Kuriyama et al 2004b;Moura et al 2012;Mikuła et al 2015a) have been investigated, besides PGRs. These reports focus on the type of explant donor (etiolation, age, length), as well as physical (light conditions, pH, various culture systems: dish, shake, immobilized, airlift) and chemical factors (sucrose and mineral salt concentrations).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Integration of tissue culture and cryopreservation methods for propagation and conservation of the fern Osmunda regalis L.

et al. 2015

View full text Add to dashboard Cite

A simple and reliable technique for in vitro multiplication and long-term preservation using liquid nitrogen was developed for gametophytes of Osmunda regalis. The effect of Knop's medium and various concentrations (1/2, 1/4, 1/8) of mineral salts provided in Murashige and Skoog (MS) basal medium, together in the presence or absence of both ammonium nitrate and a full complement of vitamins on gametophyte proliferation and sporophyte production was determined. Moreover, the effectiveness of gametophyte cryopreservation by vitrification and encapsulation-vitrification techniques was assessed. Maximum gametophyte proliferation (89 %) occurred on the ammonium nitrate-and vitamin-free [(-NH4NO3)(-vit)] MS medium with 1/2 or 1/4 strength mineral salts. The maximum production of sporophytes (30 plantlets per gametophyte clump) required 1/8MS (-NH4NO3)(-vit) medium. The flow cytometric analysis revealed that the sporophytes contained twofold more pg DNA than gametophytes. This confirmed that the sporophytes were obtained by sexual reproduction. The vitrification protocol and PVS2 solution were ineffective for cryopreservation. The greatest survival rate (81.6 %) following cryo-exposure occurred following treatment of encapsulated gametophytes with PVS3 solution for 3 h. This protocol allowed the recovery of gametophyte cultures following 6 weeks after rewarming. Finally, 100 % of sporophytes produced in vitro were successfully acclimated to ex vitro conditions. Application of the in vitro and cryopreservation methods made it possible to improve the number and time of O. regalis sporophyte production. Whole system of micropropagation can be completed in approximately 1 year. The protocols open new avenues for the mass propagation, germplasm conservation and resource management of the species.

show abstract

Section: Mass Multiplication Of Fern Gametophytesmentioning

confidence: 83%

Section: Control Of Sexual Reproduction In Fernsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Integration of tissue culture and cryopreservation methods for propagation and conservation of the fern Osmunda regalis L.

et al. 2015

View full text Add to dashboard Cite

show abstract

“…However, the requirements of sucrose and macronutrient for shoot and bulb growth of wild garlic has not been reported. Wu et al (2010) reported the effects of light, macronutrients strength and sucrose in MS medium on the spore germination and gametophyte development of the endangered fern Adiantum reniforme var. sinense.…”

Section: Introdutionmentioning

confidence: 99%