Effects of light availability on the biomass production, CO2 fixation, and bioethanol production potential of Thermosynechococcus CL-1

Su, Chih Ming; Hsueh, Hsin Ta; Li, Tzu Ying; Huang, Li; Chu, Yi Ling; Tseng, Chi Ming; Chu, Hsin

doi:10.1016/j.biortech.2013.02.092

Cited by 15 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The information would be valuable for developing feasible solutions to mitigate the impacts of global warming ( Matos et al, 2021 ). Additionally, the thermostable enzymes and bioproducts of these bacteria have been utilized in numerous scientific research and biotechnology applications ( Leu et al, 2013 ; Su et al, 2013 ; Liang et al, 2019 ; Patel et al, 2019 ; Zuliani et al, 2021 ), which further illustrates the importance of these organisms.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis reveals distinctive genomic features of Taiwan hot-spring cyanobacterium Thermosynechococcus sp. TA-1

et al. 2022

View full text Add to dashboard Cite

Thermosynechococcus is a genus of thermophilic unicellular cyanobacteria that dominates microbial mats in Asian non-acidic hot springs. These cyanobacteria are the major primary producers in their ecological niches and are promising sources of thermostable enzymes for biotechnology applications. To improve our understanding of these organisms, we conducted whole-genome sequencing of a novel strain for comparative analysis with other representatives in the same genus. This newly characterized strain, Thermosynechococcus sp. TA-1, was isolated from the Taian hot springs in Taiwan. Analyses based on average nucleotide identity (ANI) and genome-scale phylogeny suggested that TA-1 and another Taiwanese strain CL-1 belong to a novel species-level taxon. Two metagenome-assembled genomes (MAGs) originated from India represent the sister group, and Thermosynechococcus elongatus PKUAC-SCTE542 from China is the next closest lineage. All cultivated strains and MAGs from Japan form a separate monophyletic clade and could be classified into two species-level taxa. Intriguingly, although TA-1 and CL-1 share 97.0% ANI, the genome alignment identified at least 16 synteny breakpoints that are mostly associated with transposase genes, which illustrates the dynamic nature of their chromosomal evolution. Gene content comparisons identified multiple features distinct at species- or strain-level among these Thermosynechococcus representatives. Examples include genes involved in bicarbonate transportation, nitric oxide protection, urea utilization, kanamycin resistance, restriction-modification system, and chemotaxis. Moreover, we observed the insertion of type II inteins in multiple genes of the two Taiwanese strains and inferred putative horizontal transfer of an asparagine synthase gene (asnB) associated with exopolysaccharides gene cluster. Taken together, while previous work suggested that strains in this genus share a highly conserved genomic core and no clear genetic differentiation could be linked to environmental factors, we found that the overall pattern of gene content divergence is largely congruent with core genome phylogeny. However, it is difficult to distinguish between the roles of phylogenetic relatedness and geographic proximity in shaping the genetic differentiation. In conclusion, knowledge of the genomic differentiation among these strains provides valuable resources for future functional characterization.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparative analysis reveals distinctive genomic features of Taiwan hot-spring cyanobacterium Thermosynechococcus sp. TA-1

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In terms of types of bioreactors, CL-1 was applied to a continuous column cultivation system with 1.7 g/L/d of the highest cell mass productivity obtained (Su et al, 2012). The growth of CL-1 seems to be limited by illumination because its cell mass productivity can reach about 2.8 g/L/d under a 1.5-cm light path flatplate photobioreactor and optimal biomass concentration (about 3 g/L) (Su et al, 2013). After modifying the composition ratios of medium, the cell mass productivity, CO 2 fixation rate, and carbohydrate productivity can be up to 3.3, 5.3, and 1.8 g/L/d, respectively (Su et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomic Analysis of a Novel Strain of Taiwan Hot-Spring Cyanobacterium Thermosynechococcus sp. CL-1

et al. 2020

View full text Add to dashboard Cite

Thermosynechococcus is a genus of thermophilic unicellular cyanobacteria that are dominant in microbial mats at about 50-65 • C in alkaline hot springs of eastern Asia. We used PacBio SMRT Sequencing to sequence the complete genome of a novel strain of thermophilic cyanobacterium, Thermosynechococcus sp. CL-1, isolated from the Chin-Lun hot spring (pH 9.3, 62 • C) in Taiwan. Genome-scale phylogenetic analysis and average nucleotide identity (ANI) results suggested that CL-1 is a new species in the genus Thermosynechococcus. Comparative genome analysis revealed divergent genome structures of Thermosynechococcus strains. In addition, the distinct genetic differences between CL-1 and the other Thermosynechococcus strains are related to photosynthesis, transporters, signal transduction, the chaperone/usher system, nitric oxide protection, antibiotic resistance, prokaryotic immunity systems, and other physiological processes. This study suggests that Thermosynechococcus strains have actively acquired many putative horizontally transferred genes from other bacteria that enabled them to adapt to different ecological niches and stressful conditions in hot springs.

show abstract

“…Furthermore, because of their simple structures, prokaryotic cyanobacteria are suitable microorganisms for genetic modifications . However to date, only few studies have reported about the potential usage of cyanobacteria for bioethanol production .…”

Section: Introductionmentioning

confidence: 99%

Usage of thermophilic cyanobacterial biomass for bioethanol production

Karatay

2014

Env Prog and Sustain Energy

View full text Add to dashboard Cite

Although the usage of microalgae biomass as a feedstock for bioethanol production has been extensively studied, very little attention has been given to the usage of cyanobacterial biomass for bioethanol production. In this study, a thermophilic cyanobacterial strain namely Phormidium sp. was used for producing bioethanol. Some important parameters for bioethanol production were optimized. When the initial sugar concentration was 8.69 at 40 g L 21 cyanobacteria loading, Pichia stipitis used 31.5% of the sugar and produced 1.64 g L 21 ethanol and Saccharomyces cerevisiae used 34.6% of the sugar and produced 1.97 g L 21 ethanol at the end of 72 h. The highest bioethanol concentration was obtained as 2.32 g L 21 for S. cerevisiae and 1.7 g L 21 for P. stipitis at 24 h of incubation time and substrate concentration of 40 g L 21 cyanobacterial biomass at pH 5. This study demonstrated that the biomass of a thermophilic cyanobacteria is a promising feedstock for bioethanol production.

show abstract

Effects of light availability on the biomass production, CO2 fixation, and bioethanol production potential of Thermosynechococcus CL-1

Cited by 15 publications

References 10 publications

Comparative analysis reveals distinctive genomic features of Taiwan hot-spring cyanobacterium Thermosynechococcus sp. TA-1

Comparative analysis reveals distinctive genomic features of Taiwan hot-spring cyanobacterium Thermosynechococcus sp. TA-1

Comparative Genomic Analysis of a Novel Strain of Taiwan Hot-Spring Cyanobacterium Thermosynechococcus sp. CL-1

Usage of thermophilic cyanobacterial biomass for bioethanol production

Contact Info

Product

Resources

About