Effects of light and temperature on open cultivation of desert cyanobacterium Microcoleus vaginatus

Lan, Shubin; Wu, Li; Zhang, Delu; Hu, Chunxiang

doi:10.1016/j.biortech.2015.02.002

Cited by 62 publications

(27 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…submitted to a liquid‐culture based scale‐up approach, invariably had low yields or no growth at all, even if we used variations in incubation conditions that included light exposure, temperature, nutrient concentration, shaking intensity, or adding glass beads. This was surprising because mass growth of Microcoleus vaginatus has been reported in a greenhouse setting (Wang et al ; Zheng et al ), and in open‐raceway facilities (Chen et al ; Xie et al ; Lan et al , ), although no details on cultivation were given, nor any quality control of the final product usually reported (with the exception of Lan et al ), but also no reports on cultivation difficulties. In our experiment, all 32 Microcoleus spp.…”

Section: Resultsmentioning

confidence: 99%

“…Velasco Ayuso et al ; Sorochkina et al ), or open cultivation systems for either cyanobacteria (i.e. Chen et al ; Xie et al ; Lan et al , ) or mosses (Antoninka et al ). The tight control on the product, its known genetic pedigree, coupled to the strictly local isolation of cyanobacterial strains ensures that only local genetic stock is introduced to soils.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Nursing biocrusts: isolation, cultivation, and fitness test of indigenous cyanobacteria

Giraldo-Silva

Nelson

Barger

et al. 2019

Restoration Ecology

View full text Add to dashboard Cite

Biological soil crusts (biocrust) are microbial communities that develop at the soil surface of drylands and play an important role in erosion control and fertility. Soil surface disturbance from a broad range of natural and human processes (e.g. fire, livestock grazing, off-road traffic) cause significant losses in biocrust cover and associated ecosystems services. Hence, biocrust restoration is emerging as an important intervention strategy to rehabilitate degraded dryland soils. In a multistep process, we designed protocols for the establishment of "microbial nurseries" to produce photosynthetic cyanobacterial inoculum for biocrust seeding at scale. We first report on the strategy for isolation, directly from the target site, of a large culture collection of cyanobacteria that included multiple representatives of the five most common biocrust taxa. After genetic pedigreeing of these isolates, we could select those that best matched field populations genetically for scale-up cultivation. We then developed protocols for effective cyanobacterial biomass production to obtain sufficient inoculum. This was followed by conditioning treatments (hardening off) to preacclimate this inoculum to the stressful conditions expected in the field. Finally, we show that the inoculum obtained was fit to thrive in its original soil under natural outdoor conditions if sufficient water was available. We repeated this process successfully for four sites, two in the hot Chihuanuan desert and two in the cooler Great Basin Desert, and on two textural types of soils in each. The cyanobacterial biocrust nursery approach represents a versatile, viable, and safe tool for the rehabilitation of dryland soils.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Nursing biocrusts: isolation, cultivation, and fitness test of indigenous cyanobacteria

Giraldo-Silva

Nelson

Barger

et al. 2019

Restoration Ecology

View full text Add to dashboard Cite

show abstract

“…To harvest microalgal biomass is an important link to resource utilization, and sufficient biomass would be the great guarantee for microalgal resource utilization [10,26]. Therefore, the four isolated crust microalgae were further cultivated to determin their biomass accumulation.…”

Section: Microalgal Indentification and Cultivationmentioning

confidence: 99%

“…In the present study, the calculated CN Microalgal cultivation place is not only directly related to the cultivation cost, but also reflects the rationality of land use. Therefore, desert lands are proposed as the ideal microalgal cultivation place due to the abundant light resource and lower land cost [10,35]. In desert regions, environment and economy are two prominent problems hinder the local social development.…”

Section: Biodiesel Production Associated With Desertification Controlmentioning

confidence: 99%

“…Lots of the lipid-producing microalgae in the culture collection libraries have undergone long-time moderate environments, and it is difficult to adapt well to the field changeable environmental conditions when they are cultivated on a large scale [9,10]. Therefore, it becomes an important issue to directly isolate excellent lipid-producing microalgae from harsh environments, so that the microalgae can adapt well to the cultivation environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Resource utilization of microalgae from biological soil crusts: Biodiesel production associated with desertification control

Lan

Zhang

et al. 2018

Biomass and Bioenergy

Self Cite

View full text Add to dashboard Cite

With the continuing consumption of resources and increasingly prominent environmental issues, microalgal resource utilization has received extensive attention. In this study, based on the microalgal investigation in desert biological soil crusts (BSCs) using pyrosequencing technology, the cultivated crust microalgae were further isolated in order to obtain high quality microalgae for resource utilization. The results environments, but also provide ideal places for the local microalgal resource exploitation, further promoting desert socioeconomic development.

show abstract

Inoculation concentration modulating the secretion and accumulation pattern of exopolysaccharides in desert cyanobacterium Microcoleus vaginatus

Qian

Yang

et al. 2020

Biotech and App Biochem

Self Cite

View full text Add to dashboard Cite

Cyanobacterial exopolysaccharides (EPS) accumulated during microalgal cultivation have significant application potential in antioxidation, pharmaceutical products, and so on. Inoculation concentration strongly affects the cultivation cost, biomass, and EPS accumulation. In this study, a high‐EPS‐excreted desert cyanobacterium Microcoleus vaginatus was isolated, and the effects of inoculation concentration on biomass, photosynthetic activity, and EPS accumulation were explored. The results showed that the original fluorescence (Fo) provided a good indication to cyanobacterial biomass, when Chl‐a concentration was lower than 10 mg L−1. Inoculation concentration significantly affected cyanobacterial biomass and EPS concentration (P < 0.001), whereas did not affect photosynthetic activity (Fv/Fm; P > 0.05). The two fractions of EPS, capsular exopolysaccharides (CPS) and released exopolysaccharides (RPS) were strongly affect by inoculation concentration. Other than forming thick sheath (CPS) surrounded the filaments, M. vaginatus excreted higher proportions of RPS to culture medium, and the ratio of RPS to CPS ranged from 1.08 to 1.58 depending on the inoculation concentration. Additionally, although the biomass and EPS accumulation increased with inoculation concentration, the increasing inoculation concentration did not bring to the proportionate increase of the final biomass and EPS yield. Altogether, comprehensively considering the EPS yield and productivity, inoculation concentration of 0.04 mg Chl‐a L−1 is recommended for M. vaginatus to produce EPS, with an EPS yield of 94.32 mg L−1 and EPS productivity of 184.86 mg (mg Chl‐a)−1 L−1 d−1 at the end of experiment.

show abstract

Effects of light and temperature on open cultivation of desert cyanobacterium Microcoleus vaginatus

Cited by 62 publications

References 32 publications

Nursing biocrusts: isolation, cultivation, and fitness test of indigenous cyanobacteria

Nursing biocrusts: isolation, cultivation, and fitness test of indigenous cyanobacteria

Resource utilization of microalgae from biological soil crusts: Biodiesel production associated with desertification control

Inoculation concentration modulating the secretion and accumulation pattern of exopolysaccharides in desert cyanobacterium Microcoleus vaginatus

Contact Info

Product

Resources

About