Effects of light and hydrogen peroxide on gene expression of newly identified antioxidant enzymes in the harmful algal bloom species <i>Chattonella marina</i>

Mukai, Koki; Shimasaki, Yohei; Qiu, Xuchun; Kato-Unoki, Yoko; Chen, Kun; Khanam, Mst Ruhina Margia; Oshima, Yuji

doi:10.1080/09670262.2019.1576062

Cited by 16 publications

(4 citation statements)

References 63 publications

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“…In this study, P-starvation induced the overexpression of transcripts coding for a mitochondrial peroxiredoxin (10.26-fold), a bifunctional catalase-peroxidase (5.34-fold), a LINE-1 reverse transcriptase-like (4.25-fold) and a bifunctional polynucleotide phosphatase kinase (6.42-fold). Peroxiredoxin reduces hydrogen peroxide via the oxidation of a redox-active cysteine residue [57] and its expression levels were up-regulated by light-induced stress in the raphidophyte Chattonella marina [58] and increased in Chlamydomonas reinhardtii in iron starvation conditions [59]. Bifunctional catalase-peroxidase, while mainly found in bacteria [60], was also characterized in some microalgae, such as the dinoflagellate Prorocentrum minimum [61] and the green alga Volvox carteri f. nagariensis (GenBank accession no.…”

Section: Degs Related To Cellular Stressmentioning

confidence: 99%

De novo Transcriptome of the Non-saxitoxin Producing Alexandrium tamutum Reveals New Insights on Harmful Dinoflagellates

et al. 2020

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Many dinoflagellates species, especially of the Alexandrium genus, produce a series of toxins with tremendous impacts on human and environmental health, and tourism economies. Alexandrium tamutum was discovered for the first time in the Gulf of Naples, and it is not known to produce saxitoxins. However, a clone of A. tamutum from the same Gulf showed copepod reproduction impairment and antiproliferative activity. In this study, the full transcriptome of the dinoflagellate A. tamutum is presented in both control and phosphate starvation conditions. RNA-seq approach was used for in silico identification of transcripts that can be involved in the synthesis of toxic compounds. Phosphate starvation was selected because it is known to induce toxin production for other Alexandrium spp. Results showed the presence of three transcripts related to saxitoxin synthesis (sxtA, sxtG and sxtU), and others potentially related to the synthesis of additional toxic compounds (e.g., 44 transcripts annotated as “polyketide synthase”). These data suggest that even if this A. tamutum clone does not produce saxitoxins, it has the potential to produce toxic metabolites, in line with the previously observed activity. These data give new insights into toxic microalgae, toxin production and their potential applications for the treatment of human pathologies.

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Section: Degs Related To Cellular Stressmentioning

confidence: 99%

De novo Transcriptome of the Non-saxitoxin Producing Alexandrium tamutum Reveals New Insights on Harmful Dinoflagellates

et al. 2020

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“…Further it may be selective for killing Microcystis sp.. Many of the studies published on the efficacy of H 2 O 2 were conducted in a laboratory ( Buley et al, 2023 ; Chen et al, 2021 ; Guo et al, 2021 ; Mukai et al, 2019 ), so the effect might differ in field applications.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen peroxide as a mitigation against Microcystis sp. bloom

Ng,

Cheng,

Man

et al. 2023

Aquaculture

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“…We, therefore, investigated the induction of the genes that code for the production of 2-CysPrx and five other antioxidant enzymes (Cu Zn superoxide dismutase (Cu/Zn-SOD), glutathione peroxidase (GPX), catalase (CAT), ascorbate peroxidase (APX), and thioredoxin (TRX)) under different light, oxidative stress, and temperature (2-CysPrx only) conditions using quantitative PCR [ 51 , 57 , 58 ]. In dark-acclimated cells, expression levels of all antioxidant enzymes except APX were significantly increased by light irradiation (100 μmol photons m −2 s −1 ), and these expression levels decreased within 24 h, whereas the expression of the 2-CysPrx gene remained high for 24 h [ 57 ]. However, in C. marina cells acclimated to 100 μmol photons m −2 s −1 , only the levels of expression of the 2-CysPrx gene were significantly ( p < 0.05) increased by strong light irradiation (1000 μmol photons m −2 s −1 ) ( Figure 4 ).…”

Section: Induction Of Gene Expression For the Production Of Antioxmentioning

confidence: 99%

“… Conceptual diagram of gene expression pattern of six antioxidant enzymes (2-CysPRX, Cu Zn superoxide dismutase (Cu/Zn-SOD), glutathione peroxidase (GPX), catalase (CAT), and thioredoxin (TRX)) in Chattonella marina under conditions of elevated irradiance. The numbers in parentheses are light intensities (μmol photons m −2 s −1 ) [ 57 ]. …”

Section: Figurementioning

confidence: 99%

Recent Progress in the Study of Peroxiredoxin in the Harmful Algal Bloom Species Chattonella marina

et al. 2021

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Peroxiredoxin (Prx) is a relatively recently discovered antioxidant enzyme family that scavenges peroxides and is known to be present in organisms from biological taxa ranging from bacteria to multicellular eukaryotes, including photosynthetic organisms. Although there have been many studies of the Prx family in higher plants, green algae, and cyanobacteria, few studies have concerned raphidophytes and dinoflagellates, which are among the eukaryotic algae that cause harmful algal blooms (HABs). In our proteomic study using 2-D electrophoresis, we found a highly expressed 2-Cys peroxiredoxin (2-CysPrx) in the raphidophyte Chattonella marina var. antiqua, a species that induces mass mortality of aquacultured fish. The abundance of the C. marina 2-CysPrx enzyme was highest in the exponential growth phase, during which photosynthetic activity was high, and it then decreased by about a factor of two during the late stationary growth phase. This pattern suggested that 2-CysPrx is a key enzyme involved in the maintenance of high photosynthesis activity. In addition, the fact that the depression of photosynthesis by excessively high irradiance was more severe in the 2-CysPrx low-expression strain (wild type) than in the normal-expression strain (wild type) of C. marina suggested that 2-CysPrx played a critical role in protecting the cell from oxidative stress caused by exposure to excessively high irradiance. In the field of HAB research, estimates of growth potential have been desired to predict the population dynamics of HABs for mitigating damage to fisheries. Therefore, omics approaches have recently begun to be applied to elucidate the physiology of the growth of HAB species. In this review, we describe the progress we have made using a molecular physiological approach to identify the roles of 2-CysPrx and other antioxidant enzymes in mitigating environmental stress associated with strong light and high temperatures and resultant oxidative stress. We also describe results of a survey of expressed Prx genes and their growth-phase-dependent behavior in C. marina using RNA-seq analysis. Finally, we speculate about the function of these genes and the ecological significance of 2-CysPrx, such as its involvement in circadian rhythms and the toxicity of C. marina to fish.

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Effects of light and hydrogen peroxide on gene expression of newly identified antioxidant enzymes in the harmful algal bloom species Chattonella marina

Cited by 16 publications

References 63 publications

De novo Transcriptome of the Non-saxitoxin Producing Alexandrium tamutum Reveals New Insights on Harmful Dinoflagellates

De novo Transcriptome of the Non-saxitoxin Producing Alexandrium tamutum Reveals New Insights on Harmful Dinoflagellates

Hydrogen peroxide as a mitigation against Microcystis sp. bloom

Recent Progress in the Study of Peroxiredoxin in the Harmful Algal Bloom Species Chattonella marina

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