Effects of leptin on fracture healing in rat tibia

Keri̇moğlu, Gökçen; Yuluğ, Esin; Kerimoğlu, Servet; Çıtlak, Atilla

doi:10.5606/ehc.2013.23

Cited by 13 publications

(13 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[18,19] Angiogenic processes play a crucial role in the very early stages of fracture healing, affecting both endochondral and intramembranous pathways of osteogenesis. [20][21][22] Hausman et al [23] reported that antiangiogenic agents severely impair fracture healing by causing defective granulation tissue, absence of cartilage differentiation, and endochondral ossification. VEGF activity is essential for normal angiogenesis as well as appropriate callus architecture and mineralization in response to bone injury.…”

Section: Discussionmentioning

confidence: 99%

Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur

Toğral

Arıkan

Korkusuz

et al. 2015

Eklem Hastalik Cerrahisi

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur

Toğral

Arıkan

Korkusuz

et al. 2015

Eklem Hastalik Cerrahisi

View full text Add to dashboard Cite

“…In addition, leptin in the serum can induce the osteogenic differentiation of myeloid progenitor cells in bone marrow, promote the proliferation progress of osteoblasts, and promote the mineralization capacity of bone at the fracture site peripherally [8,26,30,31]. A study by Kerimoglu et al [32] found that leptin seems to have a significant dose-dependent positive effect on the healing progress of tibial fracture in rats. The weaknesses of the study by Kerimoglu et al include not determining serum leptin levels and not performing biomechanical testing of the callus.…”

Section: Discussionmentioning

confidence: 99%

Leptin Influences Healing in the Sprague Dawley Rat Fracture Model

Liu

Cai

2017

Med Sci Monit

View full text Add to dashboard Cite

BackgroundLeptin plays a crucial role in bone metabolism, and its level is related to bone callus formation in the fracture repair process. The objective of this study was to evaluate the effect of recombinant leptin on the healing process of femoral fractures in rats.Material/MethodsForty-eight male Sprague Dawley (SD) rats with an average body weight of 389 g (range: 376–398 g) and an average age of 10 weeks were included in this animal research, and all rats were randomly divided into two major groups. Then standardized femur fracture models were implemented in all SD rats. Rats in the control group were treated with only 0.5 mL of physiological saline, and rats in the experimental group were treated with recombinant leptin 5 μg/kg/d along with the same 0.5 mL of physiological saline for 42 days intraperitoneally. At the same time, each major group was evenly divided into three parallel subgroups for each parallel bone evaluation separately at the second, fourth, and sixth weeks. Each subgroup included eight rats.ResultsThe total radiological evaluation results showed that the healing progress of femoral fracture in the experimental group was superior to that in the control group from the fourth week. At the sixth week, experimental group rats began to present significantly better femoral fracture healing progress than that of the control group rats. Results of biomechanics show the ultimate load (N) and deflection ultimate load (mm) of the experimental group rats was significantly increased compared with that of the control group rats from the fourth week.ConclusionsOur results suggest that leptin may have a positive effect on SD rat femur fracture healing.

show abstract

“…Ninety adult male SD rats (230‐250 g) were purchased from Shanghai SLAC Laboratory Animal Co., Ltd and then the tibial fracture model was constructed, as documented previously . The rats were intraperitoneally anesthetized with ketamine + xylazine (75 mg/kg and 25 mg/kg, respectively), and 10% povidone iodine solution was used for disinfection.…”

Section: Methodsmentioning

confidence: 99%

“…Ninety adult male SD rats (230-250 g) were purchased from Shanghai SLAC Laboratory Animal Co., Ltd and then the tibial fracture model was constructed, as documented previously. 15 The rats were intraperitoneally anesthetized with ketamine + xylazine (75 mg/kg and 25 mg/kg, respectively), and 10% povidone iodine solution was used for disinfection. Besides, a drill was used to make a hole (0.5 mm in diameter) at the angles in the midshaft of the tibia and tibia was exposed by a longitudinal median skin incision directly over the bone.…”

Section: Model Establishment and Grouping Of Rats With Bone Fracturementioning

confidence: 99%

HMGB1 promotes bone fracture healing through activation of ERK signaling pathway in a rat tibial fracture model

Chen

Luan

2019

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

This work was to investigate potential roles of HMGB1‐mediated ERK pathway in the healing process of bone fracture. Rat tibial fracture models were established and divided into control (rats with normal saline), HMGB1 (rats with HMGB1), and HMGB1+ PD98059 groups (rats with HMGB1 and 1 mg/kg of ERK1/2 inhibitor PD98059) with 30 rats per each. The healing of rats' fracture was observed by X‐ray films, the morphological changes of bone fractures by HE staining, the callus formation by micro‐CT and biomechanical test, and the expression of osteogenesis‐related genes, HMGB1 and ERK‐related proteins by qRT‐PCR and Western blot. Rats in the HMGB1 group was increased in X‐ray scores, peak torque, torsional stiffness, and the bone volume fraction (bone volume/total volume, BV/TV); meanwhile, those rats presented elevations in osteogenesis‐related genes and HMGB1 expressions, as well as p‐ERK/ERK ratio. However, rats in the HMGB1+ PD98059 group was significantly reduced in X‐ray score, peak torque, torsional stiffness, and BV/TV, as well as the expression of osteogenesis‐related genes and the ratio of p‐ERK/ERK, as compared to those from HMGB1 group. HMGB1 could promote the expressions of osteogenesis‐related genes and accelerate the healing process of fracture via activation of the ERK signaling pathway.

show abstract

Effects of leptin on fracture healing in rat tibia

Cited by 13 publications

References 23 publications

Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur

Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur

Leptin Influences Healing in the Sprague Dawley Rat Fracture Model

HMGB1 promotes bone fracture healing through activation of ERK signaling pathway in a rat tibial fracture model

Contact Info

Product

Resources

About