Effects of leaf properties on the counts of microbes on the leaf surfaces of wheat, rye and triticale

Tang, Guojian; Yang, Fan; Li, Xinqin; Tian, Rong; Tang, Ruxue; Xu, Liuxing; Zhang, Jianguo

doi:10.1093/femsec/fiad024

Cited by 4 publications

(1 citation statement)

References 30 publications

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“…Leaf surface area (LSA) was estimated by outlining sterile bagged leaves on grid paper using a light table to minimize disturbance of attached microbial communities. These leaves (one per tree) were then "printed" onto LB agar media with 0.004 g Amphotericin B (LB/AmB), in order to deter fungal growth [47]. Specifically, the adaxial side of each leaf was pressed gently onto the agar surface using a sterilized stirring rod, and then removed and discarded.…”

Section: Culture-based Methodsmentioning

confidence: 99%

Connecting the Riparian Phyllosphere to Water Microbial Communities in a Freshwater Stream System

Dueker,

Lansbury,

Perron

2024

Preprint

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The role that aquatic aerosols might play in inter-ecosystem exchange in freshwater riparian environments has largely been understudied. In these environments, where freshwater streams are used both as drinking water and for treated waste disposal, water features like waterfalls, downed trees, and increased streamflow can serve as bioaerosol producers. Such water features could have an important role in the bacterial colonization of surrounding surfaces, including the riparian phyllosphere. In this study, we explore the influence of a freshwater stream’s bacterial community composition and micropollution on riparian maple leaves exposed to bioaerosols produced from the stream. Using culture-based and non-culture-based techniques, we compared phylloplane microbial communities in riparian zones, adjacent non-riparian forested zones, and the surface waters of the stream. In this system, riparian zone maple leaf surfaces had higher bacterial counts than non-riparian zone trees. Using metagenomic profiling of the 16S rRNA gene, we found that, while microbial communities on leaves in both riparian zone and forested sites were diverse, riparian zone bacterial communities were significantly more diverse. In addition, we found that riparian leaf bacterial communities shared more amplicon sequence variants (ASVs) with stream bacterial communities than forest leaves, indicating that the riparian zone phyllosphere is likely influenced by bioaerosols produced from water surfaces.

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Section: Culture-based Methodsmentioning

confidence: 99%

Connecting the Riparian Phyllosphere to Water Microbial Communities in a Freshwater Stream System

Dueker,

Lansbury,

Perron

2024

Preprint

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Life on a leaf: the epiphyte to pathogen continuum and interplay in the phyllosphere

Thomas,

Kay,

Fones

2024

BMC Biol

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Epiphytic microbes are those that live for some or all of their life cycle on the surface of plant leaves. Leaf surfaces are a topologically complex, physicochemically heterogeneous habitat that is home to extensive, mixed communities of resident and transient inhabitants from all three domains of life. In this review, we discuss the origins of leaf surface microbes and how different biotic and abiotic factors shape their communities. We discuss the leaf surface as a habitat and microbial adaptations which allow some species to thrive there, with particular emphasis on microbes that occupy the continuum between epiphytic specialists and phytopathogens, groups which have considerable overlap in terms of adapting to the leaf surface and between which a single virulence determinant can move a microbial strain. Finally, we discuss the recent findings that the wheat pathogenic fungus Zymoseptoria tritici spends a considerable amount of time on the leaf surface, and ask what insights other epiphytic organisms might provide into this pathogen, as well as how Z. tritici might serve as a model system for investigating plant–microbe-microbe interactions on the leaf surface.

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Connecting Riparian Phyllospheres to Aquatic Microbial Communities in a Freshwater Stream System

Dueker,

Lansbury,

Perron

2024

Aerobiology

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The role that aquatic aerosols might play in inter-ecosystem exchanges in freshwater riparian environments has largely been understudied. In these environments, where freshwater streams are used both as drinking water and for treated waste disposal, water features like waterfalls, downed trees, and increased streamflow can serve as bioaerosol producers. Such water features could have an important role in the bacterial colonization of surrounding surfaces, including the riparian phyllosphere. In this study, we explore the influence of a freshwater stream’s bacterial community composition and micropollution on riparian maple leaves exposed to bioaerosols produced from that stream. Using culture-based and non-culture-based techniques, we compared phylloplane microbial communities in riparian zones, adjacent non-riparian forested zones, and the surface waters of the stream. In this system, riparian zone maple leaf surfaces had higher bacterial counts than non-riparian zone trees. Using metagenomic profiling of the 16S rRNA gene, we found that, while microbial communities on leaves in both the riparian zone and forested sites were diverse, riparian zone bacterial communities were significantly more diverse. In addition, we found that riparian leaf bacterial communities shared more amplicon sequence variants (ASVs) with stream bacterial communities than forest leaves, indicating that the riparian zone phyllosphere is likely influenced by bioaerosols produced from water surfaces.

show abstract

Effects of leaf properties on the counts of microbes on the leaf surfaces of wheat, rye and triticale

Cited by 4 publications

References 30 publications

Connecting the Riparian Phyllosphere to Water Microbial Communities in a Freshwater Stream System

Connecting the Riparian Phyllosphere to Water Microbial Communities in a Freshwater Stream System

Life on a leaf: the epiphyte to pathogen continuum and interplay in the phyllosphere

Connecting Riparian Phyllospheres to Aquatic Microbial Communities in a Freshwater Stream System

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