Effects of Lead and Cadmium Combined Heavy Metals on Liver Function and Lipid Metabolism in Mice

Abstract

Although a large number of studies have been conducted on individual lead (Pb) or cadmium (Cd) exposures, the toxicity of combined Pb and Cd exposures is relatively limited. The present study aims to investigate toxicity of Pb-Cd combined heavy metals and its mechanism. A heavy metal exposure model was established by subcutaneous intragastric administration of Pb-Cd (50:1) for 35 days in mice. The body weight, diet, hair color, mental state, liver index, hematological index, biochemical indicators and pathological section were used to comprehensively evaluate the toxicity reaction. Then, classical oxidative stress indexes and lipidomics techniques had been used to explore the potential mechanism. The results showed that Pb-Cd could cause inappetite, poor spirit, signi cantly reduced activity, slow weight gain and dark hair color in mice. Pb-Cd could also cause liver enlargement, AST and ALT enzyme activities signi cantly increased, and pathological changes of liver. Prolonged exposure to c could also cause PCT, WBC, PLT and MON signi cantly increased, and RBC, HGB, HCT and LYM decreased. Pb-Cd can increase the oxidative stress by increasing the activity of SOD and LDH and the content of MDA. Pb-Cd also could cause lipid metabolism disorders by regulating linoleic acid metabolism, sphingolipid metabolism and glycerolipid metabolism.

Protective effect of Lactiplantibacillus plantarum CCFM8661 against heavy metal mixture-induced liver and kidney injury in mice

“…In a study investigating the impact of lead and cadmium on liver function and lipid metabolism in mice, Chen, et al observed that mice exposed to lead and cadmium exhibited lower body weight gain compared to those in the control group. 20 The weight increase in the MC group was slower, showing a decrease of 13.37% compared to the NC group. However, intervention with L. plantarum CCFM8661 significantly improved this situation, as administration of 0.8 × 10 9 CFU L. plantarum CCFM8661 increased the weight gain by 7.11% in mice exposed to mixed heavy metals.…”

The single and mixed impacts of cadmium, cobalt, lead, and PAHs on systemic immunity inflammation index in male and female

“…According to previous reports, the results of this study are reasonable and evidence-based. Chronic exposure to Cd resulted in a significant increase in platelet counts and a decrease in lymphocyte counts in mice ( 52 ). Akbar et al showed that Co was not directly cytotoxic to lymphocytes but might affect intracellular molecular homeostasis and thus inhibit lymphocyte proliferation ( 53 ).…”

Docosahexaenoic Acid Promotes Cd Excretion by Restoring the Abundance of Parabacteroides in Cd-Exposed Mice

“…We also found that DHA can effectively reduce the activities of serum AST and ALT and restore the integrity of liver tissue. Cd is transported through the blood to various organs, and liver damage caused by Cd leads to increased membrane fluidity, causing enzymes in the liver to leak into the blood [ 36 ]. Therefore, serum AST and ALT are important markers of hepatocyte injury [ 37 , 38 ].…”