Effects of lathyrism on mouse molar migration

Abstract

Previous cursory reports of lathyritic aiveoiar bone activity prompted a histotogic and radioautographic investigation of tooth migration patterns in normal and lathyritic mice. Using Sharpey's fiber patterns and the distribution of (^H)-proUne radioactive label as indicators, bone deposition was found on distal, occlusal, and buccal facing alveolar surfaces and revealed normal tooth migration to be in a distal, occlusal, and buccal direction. In addition, the radioautographic label confirmed the reliability of using Sharpey's fiber patterns as directional indicators. Over a forty-day lathyritic period a progressive disturbance of Sharpey/principal fiber attachment at the bone surface plus radioautographic inactivity indicated that the distal and buccal vectors of migration had ceased. However, occlusal migration appeared to continue. These results were considered to be a likely consequence of the duration and severity of the iathyritic change and the difference in turnover times between the supracrestal and periodontai ligament collagen fibers, with further consideration to the occlusion, ultrastructural, and vasailar changes being required.

The distribution of 3H‐proline in alveolar bone of the mouse as seen by radioautography

“…A low but significant level of plasma radioactivity persists for several hours (Josephsen and Warshawsky, 1982). In the present study, 3H-proline was incorporated into both bone matrix and Sharpey's (extrinsic) fibers, a situation previously described by Garant and Cho (1979) and by Dreyer and Sampson (1984). New bone formation on the distal wall of the septum entrapped some periodontal ligament fibers which became Sharpey's fibers of alveolar bone.…”

“…Formation and "migration" of the appositional band in bone are reported by several authors (Stallard, 1963;Crumley, 1964;Garant and Cho, 1979;Dreyer and Sampson, 1984) and are suggested to be useful as a method for determination of bone growth (Kenney and Ramfjord, 1969;Tonna, 1975Tonna, , 1976. This band is likely a direct reflection of the amount of label injected initially (Josephsen and Warshawsky, 1982).…”

“…Osteocytes also produce a small amount of matrix on their lacunar walls (Wassermann and Yaeger, 1965;Baylink and Wergedal, 1971;Jande, 1971Jande, , 1972Baud, 1968;Baud and Auil, 1971; Belanger, 1971, 1973;Zambonin Zallone et al, 1983). Bone is resorbed primarily by osteoclasts and by osteocytes, the latter process being termed osteocytic osteolysis (Belanger, 1969;Baylink et al, 1973;Weisbrode et al, 1974;Parafitt, 1976).Incorporation of 3H-proline into alveolar bone is an important method in the study of the effects of physiologic tooth movement on alveolar bone structure (Stallard, 1963;Crumley, 1964;Baumhammers et al, 1965;Carneiro and Fava de Moraes, 1965;Anderson, 1967;Kenney and Ramfjord, 1969;Tonna, 1975Tonna, ,1976Garant and Cho, 1979;Dreyer and Sampson, 1984). Injection of 3H-proline into a n animal results in creation of a radioactive front resulting from the initial pulse of the label which, in bone, is transferred into a record of labeled collagenous proteins produced by bone synthetic cells (Carneiro and Leblond, 1959;Weinstock and Leblond, 1974;Weinstock, 1975;Leblond and Wright, 1980 ever, labeled bone continues to be formed, likely a direct reflection of the amount of radioactive proline remaining in the body (Josephsen and Warshawsky, 1982).…”

“…The processes of bone formation and resorption have been outlined in some detail by various authors. In the alveolar bone of rodents, it has been well established that new bone is formed on the distal wall and the crest of the interdental septum (Stallard, 1963;Crumley, 1964;Baumhammers et al, 1965;Carneiro and Fava de Moraes, 1965;Anderson, 1967; Kenney and Ramfjord, 1969;Tonna, 1975Tonna, , 1976; Garant and Cho, 1979; Dreyer and Sampson, 1984). Bone resorption occurs on the medial wall of the septum.…”

“…Incorporation of 3H-proline into alveolar bone is an important method in the study of the effects of physiologic tooth movement on alveolar bone structure (Stallard, 1963;Crumley, 1964;Baumhammers et al, 1965;Carneiro and Fava de Moraes, 1965;Anderson, 1967; Kenney and Ramfjord, 1969;Tonna, 1975Tonna, ,1976; Garant and Cho, 1979;Dreyer and Sampson, 1984). Injection of 3H-proline into a n animal results in creation of a radioactive front resulting from the initial pulse of the label which, in bone, is transferred into a record of labeled collagenous proteins produced by bone synthetic cells (Carneiro and Leblond, 1959;Weinstock and Leblond, 1974;Weinstock, 1975; Leblond and Wright, 1980).…”