Effects of laser processing conditions on wettability and proliferation of Saos-2 cells on CoCrMo alloy surfaces

Abstract

Any processing disturbances in laser surface texturing (LST) could compromise the resulting surface topography and their desired functional response. Disturbances such as focal plane offsets and beam incident angle variations are always present in LST processing of 3D parts and can affect the surface morphology. In this research the effects of these laser processing disturbances in producing laser induced surface structures (LIPSS) on CoCrMo alloy substrates were investigated. In particular, these two disturbances were considered as laser processing variables to determine their effects on functional responses of LIPSS treated surfaces, i.e. surface wettability and the proliferation of Saos-2 osteoblast-like cells were evaluated. It was found that the changes of laser processing conditions led to a decrease in surface wettability and Saos-2 cells proliferation. In addition, a correlation between surface wettability and cell proliferation on LIPSS treated surface was identified and conclusions made about the effects of investigated process disturbances on the functional response of LIPSS treated CoCrMo substrates.

Anti-Biofouling Properties of Femtosecond Laser-Induced Submicron Topographies on Elastomeric Surfaces

“…In particular, two types of LIPSS topographies were fabricated on steel masters using femtosecond laser processing. The first type was highly regular LIPSS with a periodicity of 800-900 nm [38][39] while the second was a multiscale (MS) topography, i.e. LIPSS superimposed on micron-scale protrusions.…”

Laser processing of freeform surfaces: A new approach based on an efficient workpiece partitioning strategy

“…Therefore, the effects of processing disturbances, i.e. deviations of the BIA from normal and off-focus processing, should be taken into account when processing strategies are designed, especially when partitioning 3D surfaces into laser processing fields [14]. A novel partitioning method is proposed in this research that employs an efficient strategy for tessellating 3D surfaces.…”

“…Most laser surface processing research was conducted on planar substrates, however, processing disturbances are present when processing complex shapes, such as the acetabular shells found in total hip replacements [14]. This spherical component can be produced to near net shape by LPBF.…”

“…In particular, variations in the beam incidence angle (BIA) and focal offset distance (FOD) when processing 3D surfaces directly affect the process performance. Therefore, these two factors should be taken into account when deciding how to partition such surfaces for laser processing [14].…”

Functionalization of Plastic Parts by Replication of Variable Pitch Laser-Induced Periodic Surface Structures

“…Then, the surface of the inserts was treated with ultrashort laser patterning using a femtosecond laser source (Satsuma, Amplitude Systems, Pessac, France). The laser process parameters, reported in Table 1, were selected based on a previous optimization study to obtain a uniform pitch and depth of the ripples [39]. The laser beam polarization was unchanged during the trials, and the inserts were oriented identically in the Computer Numerical Control (CNC) machining setup, ensuring the same ripples' directions over the four textured samples.…”