Effects of Laser Photobiomodulation on Cutaneous Wounds Treated with Mitomycin C: A Histomorphometric and Histological Study in a Rodent Model

Santos, Nicole Ribeiro Silva; Santos, Jean Nunes dos; Sobrinho, João Batista de Macedo; Ramalho, Luciana Maria Pedreira; Carvalho, Carolina Montagn; Soares, Luiz Guilherme P.; Pinheiro, António Luiz Barbosa

doi:10.1089/pho.2008.2380

Cited by 11 publications

(5 citation statements)

References 16 publications

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“…We found that the use of laser and polarized light are effective on improving the healing of diabetic 19,20 and malnourished animals. 21,22 We also found that laser light is capable of improving the healing of drug-induced impairment 23 and on increasing the survival rate of musculocutaneous flaps on both diabetic and nondiabetic animals. 24 We have also studied and shown the influence of laser parameters on the healing of surgical wounds 25,26 as well as on the postoperative period of both surgical and CO 2 laser wounds.…”

mentioning

confidence: 68%

“…In this case, we have reduction in tissue necrosis and less and precocious inflammatory reaction. 23 In cases in which some therapeutical approach may led to impairment of the healing, such as the use of some drugs, the use of LPT resulted in less inflammation and increased fibroblast proliferation and collagen deposition, favoring healing. 24 Our group has shown, using different models, that association of bone grafts, bone morphogenetic proteins, and guided tissue regeneration does improve the healing of bone tissue.…”

Section: Bone Repairmentioning

confidence: 99%

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Advances and Perspectives on Tissue Repair and Healing

Pinheiro

2009

Photomedicine and Laser Surgery

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W ound healing is a complex process that involves both local and systemic responses, and these responses will widely reflect the etiology of the lesion, type of tissue, systemic condition of the subject, etc. Despite being essentially the same for different wounds, the pattern of healing may change due to both intrinsic and=or extrinsic factors. These factors include age, gender, hormonal factors, nutritional deficiencies, the use of drugs, and some diseases. It is well known that some diseases or conditions such as uncontrolled diabetes, malnutrition, hypothyroidism, anemia, burns, etc., may cause delayed wound healing. The type of tissue has also to be considered as, despite showing similar phases, skin and mucosal wounds heals quickly than bone. Several therapeutic approaches have been used to improve healing, and these include the use of stem cells, 1 growth factors, 2 drugs, 3 hyperbaric oxygen, 4 and phototherapies. 5-8 Several light therapies have been used to improve both healing and repair. Laser, light emitting diodes (LEDs), and lamps have been used worldwide, both experimentally and clinically, to improve healing. It has been shown elsewhere that different phototherapies are able to stimulate the repair of skin, ligament, tendon, bone, and cartilage in laboratory animal wounds and human ulcers.

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mentioning

confidence: 68%

Section: Bone Repairmentioning

confidence: 99%

Advances and Perspectives on Tissue Repair and Healing

Pinheiro

2009

Photomedicine and Laser Surgery

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“…Red light induces differential alterations in 76 genes in wounds treatment 28 . It mainly exerts repairing effects by regulating inflammatory factors, cell growth factors, ROS, and energy production metabolism 29 , 30 . This is mainly because red light itself produces photons that are less likely to scatter and more likely to penetrate tissues.…”

Section: Discussionmentioning

confidence: 99%

Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction

Gao¹,

Li²,

Zhang³

et al. 2022

Int. J. Biol. Sci.

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Photobiomodulation (PBM) has emerged as an alternative therapy involved in modulating a variety of biological effects. In this study, we verified whether PBM can affect cardiac physiological activity in mice through noninvasive irradiation using light-emitting diodes at a wavelength of 630 nm (LED-Red). We found that the PBM involved in regulating the repair of injured myocardium is wavelength-limited. LED-Red caused cardiomyocytes (CMs) that had exited the cell cycle to divide and proliferate again, and the cell proliferation ratio increased significantly with the accumulation of intracellular photopower. In addition, LED-Red promoted myocardial revascularization and myocardial regeneration, reduced the area of fibrosis in mice with myocardial infarction (MI), and thus improved cardiac contractile function. In regard to the mechanism, miRNA sequencing analysis showed that low-power LED-Red irradiation could induce differential changes in miRNAs in CMs. Among them, miR-136-5p was identified as a cardiac photo-sensitive miRNA and was obviously inhibited after stimulation, which produced a proliferation-promoting effect on CMs. Subsequent luciferase reporter assays confirmed the involvement of Ino80 as a binding target of miR-136-5p in the regulatory process of CM proliferation. Similarly, LED-Red irradiation elevated intracellular Ino80 expression. After knockdown of Ino80, the proliferation-promoting effect of LED-Red on CMs was inhibited. Collectively, this study demonstrates that LED-Red can promote CM proliferation by inhibiting cardiac photo-sensitive miRNA- miR-136-5p expression through targeting Ino80. The findings provided a new potential strategy for the treatment of ischemic cardiomyopathy (ICD).

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“…Low-Intensity Light Therapy (LILT), also known as photobiomodulation, has been reported to modulate a considerable number of biological processes in a significant number of studies. These include increasing wound healing rates [4][5][6][7]; induction of cellular proliferation in multiple cell culture systems; including osteoblasts [8][9][10], fibroblasts [11][12][13], muscle cells and epithelial cells [14,15]; improving the healing time of Herpes labialis infections [16]; increasing ATP synthesis and mitochondrial respiration rates [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Low-Intensity Light Therapy (1068 nm) Protects CAD Neuroblastoma Cells from Î²-Amyloid-Mediated Cell Death

Duggett¹,

Chazot²

2014

Biol Med (Aligarh)

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Using a simple in vitro amyloidopathy CAD neuronal model, infrared (IR) 1068 nm light treatment (5 x 3 minutes) was investigated as a novel neuroprotection strategy. Synthetic human β-amyloid (1-42) peptide was subjected to aggregation in a test-tube, and shown to form fibrils of a range of sizes (individually ~10 μm), which compromised the cellular nuclear integrity of CAD cells in culture, and elicited a dose-dependent neurotoxicity (β-amyloid (1-42) peptide concentration range 0-25 μM) up to 73%, which was significantly suppressed (up to 24%; p<0.001) by prior treatment with IR1068.

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Effects of Laser Photobiomodulation on Cutaneous Wounds Treated with Mitomycin C: A Histomorphometric and Histological Study in a Rodent Model

Abstract: The use of LPBM improves wound healing in subjects treated with MMC.

Cited by 11 publications

References 16 publications

Advances and Perspectives on Tissue Repair and Healing

Advances and Perspectives on Tissue Repair and Healing

Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction

Low-Intensity Light Therapy (1068 nm) Protects CAD Neuroblastoma Cells from Î²-Amyloid-Mediated Cell Death

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