that vonoprazan is an inhibitor of not only CYP3A4 but also CYP2C19, and the CYPmediated drug-drug interaction (DDI) between vonoprazan and clopidogrel attenuated the antiplatelet function of clopidogrel. 1 Vonoprazan is primarily cleared by CYP3A4 metabolism and its autoinhibition would be expected to result in accumulation of vonoprazan on repeat dosing. However, our clinical data showed only minor accumulation (Accumulation Index <1.2) following 10-40 mg q.d. dosing for 7 days. 2 In addition, a triple therapy with vonoprazan, amoxicillin (not a CYP3A4 inhibitor), and clarithromycin (a substrate and inhibitor of CYP3A4) showed a 1.5fold increase in AUC of clarithromycin; however, the exposure to hydroxyclarithromycin, a metabolite formed by CYP3A4, increased 1.9-fold. 3 Thus, vonoprazan did not inhibit the metabolism of clarithromycin by CYP3A4. The cause for the increase of exposure to clarithromycin was not elucidated, but could be due to gastric pH changes like those reported for lansoprazole 4 and not due to the CYP3A4 inhibition by vonoprazan. Broadly, the body of in vivo evidence does not suggest a causal relationship between exposure to vonoprazan and CYP3A4-or CYP2C19-mediated DDI with clopidogrel.Clopidogrel is known to be pharmacologically activated to metabolite H4 and its isomers by multiple CYPs, including CYP2C19 and CYP3A4. 5 In the repeat dose study of vonoprazan at 10 mg, the steady-state C max , one of the major determinants of the extent of DDIs, of vonoprazan was 35 nmol/L. 2 In vitro, vonoprazan showed no significant reversible inhibition of any of the major CYP isoforms (IC 50 16 lmol/L), but showed a time-dependent inhibition (TDI) for CYP2B6, CYP2C19, and CYP3A4. However, the TDI effects were weaker than that of the corresponding reference compounds (ticlopidine, esomeprazole, and verapamil) based on the measured k inact /K I ratios. In a more direct experiment, the inhibitory effect of vonoprazan on the in vitro metabolism of [ 14 C]clopidogrel in human liver microsomes showed that vonoprazan had no significant inhibitory effect, reversible or TDI, on the formation of H4 and its isomers at concentrations substantially higher than 35 nmol/ L, the clinical steady-state C max (Table 1), which further supported the argument against CYP3A4-and CYP2C19-mediated DDI with clopidogrel. Additionally, the assessment of clinical trials and postmarketing safety data suggested no evidence of DDI between vonoprazan and clopidogrel or prasugrel ("data on file"). Table 1 Inhibitory effect of vonoprazan on in vitro metabolism of [ 14 C]clopidogrel with/without preincubation on the formation of active metabolite H4 and its isomers Inhibitor Concentration (lmol/L) Relative activity (%) Formation of H4 and its isomers Without preincubation With preincubation Control 0 100.0 100.0 Vonoprazan 0.03 100.0 103.8 0.1 100.0 101.9 0.3 98.5 105.8 1 97.1 94.2 3 86.8 82.7 10 83.8 73.1[ 14 C]Clopidogrel (10 lmol/L) and inhibitor were incubated with human liver microsomes (1 mg protein/mL) in the presence of an NADPH ...