Effects of lactoferrin and lactoperoxidase‐containing food on the oral hygiene status of older individuals: A randomized, double blinded, placebo‐controlled clinical trial

Morita, Yoshio; Ishikawa, Kentaro; Nakano, Manabu; Wakabayashi, Hiroyuki; Yamauchi, Koji; Abe, Fumiaki; Ooka, Takafumi; Hironaka, Shouji

doi:10.1111/ggi.12776

Cited by 24 publications

(29 citation statements)

References 24 publications

(48 reference statements)

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“…In the present study, decreases in the total relative abundance of gram‐negative bacteria in supragingival plaque correlated with deterioration of oral health‐related variables, including O'Leary's PCR, BOP and total VSCs. In addition, at 8 weeks in the test group BOP had significantly decreased from baseline . These results suggest that the microbial shift induced by the test tablets from a gram‐negative‐dominant to a gram‐positive‐dominant community contributes to improvements in oral health.…”

Section: Discussionmentioning

confidence: 75%

“…The results of comparisons of the phylum composition suggest that ingestion of the test tablets does not markedly affect the overall structure of the microbiota in supragingival plaque and tongue coating. No adverse events related to the tablets were observed in any of the 46 study participants . Thus, long‐term use of the test tablets does not appear to result in microbial substitution.…”

Section: Discussionmentioning

confidence: 86%

“…Because they have these properties, LF and LPO have been used in oral health care products and foods (12,13). Morita et al have shown that long-term ingestion of LF and LPO-containing tablets significantly reduces the number of P. gingivalis and Fusobacterium nucleatum in supragingival plaque and tongue coating in older individuals (14). These findings suggest that LF and LPO have the potential to suppress several oral pathogens.…”

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confidence: 99%

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Effects of lactoferrin and lactoperoxidase‐containing food on the oral microbiota of older individuals

Nakano

Wakabayashi

Sugahara³

et al. 2017

Microbiology and Immunology

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The oral microbiota influences health and disease states. Some gram-negative anaerobic bacteria play important roles in tissue destruction associated with periodontal disease. Lactoferrin (LF) and lactoperoxidase (LPO) are antimicrobial proteins found in saliva; however, their influence on the whole oral microbiota currently remains unknown. In this randomized, double-blinded, placebo-controlled study, the effects of long-term ingestion of LF and LPO-containing tablets on the microbiota of supragingival plaque and tongue coating were assessed. Forty-six older individuals ingested placebo or test tablets after every meal for 8 weeks. The relative abundance of bacterial species was assessed by 16S rRNA gene high-throughput sequencing. Most of the bacterial species in supragingival plaque and tongue coating that exhibited significant decreases in the test group were gram-negative bacteria, including periodontal pathogens. Decreases in the total relative abundance of gram-negative organisms in supragingival plaque and tongue coating correlated with improvements in assessed variables related to oral health, such as oral malodor and plaque accumulation. Furthermore, there was significantly less microbiota diversity in supragingival plaque at 8 weeks in the test group than in the placebo group and low microbiota diversity correlated with improvements in assessed variables related to oral health. These results suggest that LF and LPO-containing tablets promote a shift from a highly diverse and gram-negative-dominated to a gram-positive-dominated community in the microbiota of supragingival plaque and tongue coating. This microbial shift may contribute to improvements in oral health, including oral malodor and state of the gingiva.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 86%

mentioning

confidence: 99%

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Effects of lactoferrin and lactoperoxidase‐containing food on the oral microbiota of older individuals

Nakano

Wakabayashi

Sugahara³

et al. 2017

Microbiology and Immunology

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“…Morita et al. () conducted a randomized, double‐blinded, placebo‐controlled clinical trial to investigate the effects of LF and lactoperoxidase (LPO)‐containing food on the oral hygiene status of a geriatric population. They found that ingestion of LF and LPO‐containing tablets for 8 weeks provided significant anti‐bacterial effects on the periodontal bacteria ( P. gingivalis and Fusobacterium nucleatum ) present in the tongue coating and supragingival plaque.…”

Section: Effect Of Lf On Periodontal Pathogensmentioning

confidence: 99%

Role of lactoferrin and lactoferrin‐derived peptides in oral and maxillofacial diseases

et al. 2018

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The oral cavity harbors different taxonomic groups, the evolutionary coexistence of which develops the oral ecosystem. These resident microorganisms can alter the balance between the physiologic and pathologic conditions that affect the host, both locally and systemically. This highly sophisticated nature of the oral cavity poses a significant therapeutic challenge. Numerous human and animal studies have been conducted to potentiate the efficacy and competence of current treatments of pathologic conditions as well as to develop novel therapeutic modalities. One of these studies is the use of the potent antimicrobial agent lactoferrin (LF), which was originally derived from the host immune system. LF is an 80-kDa glycoprotein that has a free iron sequestration mechanism with evident antimicrobial, anti-tumor, and immunomodulatory properties. A wide range of active peptides have been isolated from the N-terminal region of LF, which possess antimicrobial activities. In this review, we discuss the role of LF and LF-derived peptides under a heterogeneous group of oral and maxillofacial conditions, including bacterial, fungal, viral infections; head and neck cancers; xerostomia; and implantology-bone-related manifestations.

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“…No adverse events related to the test tablets were observed in any of the participants during the study period, consistent with the results of previous clinical studies. 17,29 Therefore, the LF and LPO-containing tablets investigated in this clinical trial may be safely used on a daily basis to maintain oral health.…”

Section: Discussionmentioning

confidence: 99%

Effect of tablets containing lactoferrin and lactoperoxidase on gingival health in adults: A randomized, double‐blind, placebo‐controlled clinical trial

Nakano

Yoshida

Wakabayashi

et al. 2019

J of Periodontal Research

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Objective To evaluate the effect of tablets containing lactoferrin (LF) and lactoperoxidase (LPO) on gingival health and oral health‐related quality of life in healthy adults. Background Lactoferrin and LPO are host defense factors found in saliva that may contribute to oral health. Materials and Methods One hundred and fifty adults were randomly assigned to the administration of high‐dose tablets (LF 60 mg/d, LPO 7.8 mg/d), low‐dose tablets (LF 20 mg/d, LPO 2.6 mg/d), or placebo tablets for 12 weeks. The gingival index (GI) and plaque index (PlI) were measured at baseline and after 12 weeks. Oral health‐related quality of life was assessed by the Oral Health Impact Profile (OHIP) at baseline and at 4, 8, and 12 weeks. Results One hundred and nine healthy subjects were included in the efficacy analysis. In the high‐dose group, the GI was significantly reduced after 12 weeks of treatment, and the reduction in GI in the high‐dose group was significant compared with the placebo group. In both the high‐dose group and the low‐dose group, PlI showed a significant decrease at 12 weeks compared with baseline. The total OHIP score was significantly reduced at 12 weeks in the high‐dose group. In addition, the OHIP functional limitation subscale displayed significant improvement in the high‐dose groups compared with the placebo group at 12 weeks. No adverse reactions or serious adverse events related to the test tablets were observed in any of participants during the study, and the incidence of adverse events unrelated to the tablets did not differ significantly among the groups. Conclusion These results suggest that intake of tablets containing LF (60 mg/d) and LPO (7.8 mg/d) can potentially improve gingival inflammation and oral health‐related quality of life in healthy adults.

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Effects of lactoferrin and lactoperoxidase‐containing food on the oral hygiene status of older individuals: A randomized, double blinded, placebo‐controlled clinical trial

Cited by 24 publications

References 24 publications

Effects of lactoferrin and lactoperoxidase‐containing food on the oral microbiota of older individuals

Effects of lactoferrin and lactoperoxidase‐containing food on the oral microbiota of older individuals

Role of lactoferrin and lactoferrin‐derived peptides in oral and maxillofacial diseases

Effect of tablets containing lactoferrin and lactoperoxidase on gingival health in adults: A randomized, double‐blind, placebo‐controlled clinical trial

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