Effects of Lactobacillus reuteri MG5346 on Receptor
                    Activator of Nuclear Factor-Kappa B Ligand (RANKL)-Induced Osteoclastogenesis
                    and Ligature-Induced Experimental Periodontitis Rats

Jeong, Yu‐Jin; Jung, Jae-In; Kim, YongGyeong; Kang, Chang-Ho; Imm, Jee-Young

doi:10.5851/kosfa.2022.e68

Cited by 6 publications

(3 citation statements)

References 43 publications

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“…Periodontitis is a typical chronic inflammatory disease that is associated with not only gingival inflammation but also alveolar bone loss. The occurrence of bone resorption indicates that homeostasis in periodontal tissue is absent under inflammatory conditions [ 38 ].The treatment effect of curcumin in periodontal disease includes anti-inflammation and relieves alveolar bone resorption. Therefore, we have further detected ferroptosis related markers in alveolar bone tissues.…”

Section: Resultsmentioning

confidence: 99%

Curcumin Attenuates Periodontal Injury via Inhibiting Ferroptosis of Ligature-Induced Periodontitis in Mice

Wang

Lin

Huang

et al. 2023

IJMS

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Periodontitis is a chronic infectious disease characterized by the destruction of connective tissue and alveolar bone that eventually leads to tooth loss. Ferroptosis is an iron-dependent regulated cell death and is involved in ligature-induced periodontitis in vivo. Studies have demonstrated that curcumin has a potential therapeutic effect on periodontitis, but the mechanism is still unclear. The purpose of this study was to investigate the protective effects of curcumin on alleviating ferroptosis in periodontitis. Ligature-induced periodontal-diseased mice were used to detect the protective effect of curcumin. The level of superoxide dismutase (SOD), malondialdehyde (MDA) and total glutathione (GSH) in gingiva and alveolar bone were assayed. Furthermore, the mRNA expression levels of acsl4, slc7a11, gpx4 and tfr1 were measured using qPCR and the protein expression of ACSL4, SLC7A11, GPX4 and TfR1 were investigated by Western blot and immunocytochemistry (IHC). Curcumin reduced the level of MDA and increased the level of GSH. Additionally, curcumin was proven to significantly increase the expression levels of SLC7A11 and GPX4 and inhibit the expression of ACSL4 and TfR1. In conclusion, curcumin plays a protective role by inhibiting ferroptosis in ligature-induced periodontal-diseased mice.

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Section: Resultsmentioning

confidence: 99%

Curcumin Attenuates Periodontal Injury via Inhibiting Ferroptosis of Ligature-Induced Periodontitis in Mice

Wang

Lin

Huang

et al. 2023

IJMS

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“…CFSs from the postbiotics Lactobacillus salivarius and Lactobacillus reuteri have considerable potential as functional oral health ingredients to inhibit alveolar bone loss associated with periodontitis [ 25 , 38 ]. Meanwhile, the W. cibaria CMU (oraCMU) and CMS1 (oraCMS1) strains have been commercialized as oral probiotics, and there has been ongoing research on their beneficial effects on oral health [ 18 , 19 , 20 , 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effects of Weissella cibaria CMU and CMS1 on Receptor Activator of NF-κB Ligand (RANKL)-Induced Osteoclast Differentiation

Park,

Kang

2024

JFB

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Excessive osteoclast activity can promote periodontitis-associated bone destruction. The inhibitory mechanisms of Weissella cibaria strains CMU and CMS1 against periodontitis have not yet been fully elucidated. In this study, we aimed to investigate whether heat-killed (HK) W. cibaria CMU and CMS1 or their respective cell-free supernatants (CFSs) inhibit osteoclast differentiation and bone resorption in response to receptor activator of nuclear factor kappa-B ligand (RANKL)-treated RAW 264.7 cells. TRAP (tartrate-resistant acid phosphatase) staining and bone resorption assays revealed that both HK bacteria and CFSs significantly suppressed the number of TRAP-positive cells, TRAP activity, and bone pit formation compared to the RANKL-treated control (p < 0.05). HK bacteria dose-dependently inhibited osteoclastogenesis while selectively regulating certain genes in CFSs (p < 0.05). We found that disrupting the direct interaction between HK bacteria and RAW 264.7 cells abolished the inhibitory effect of HK bacteria on the expression of osteoclastogenesis-associated proteins (c-Fos, nuclear factor of activated T cells c1 (NFATc1), and cathepsin K). These results suggest that dead bacteria suppress osteoclast differentiation more effectively than the metabolites and may serve as beneficial agents in preventing periodontitis by inhibiting osteoclast differentiation via direct interaction with cells.

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“…Previous studies have shown that B. lactis HN019 could reduce the expression of RANKL, but had no effect on the level of OPG [51]. In addition, L. reuteri MG5346 can signi cantly reverse the ratio of OPG to RANKL in periodontitis rats' gingival tissue [52]. Dental plaque bio lms have the capability to upregulate RANKL while downregulating OPG expression, resulting in a reduction of the OPG/RANKL ratio [53][54][55][56].…”

Section: Disscussionmentioning

confidence: 99%

Preventive and Therapeutic Potential of Streptococcus cristatus CA119 in Experimental Periodontitis in Rats

Zhao,

Li,

Pan

et al. 2024

Probiotics & Antimicro. Prot.

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Periodontitis is a chronic in ammatory disease characterized by the overgrowth of Porphyromonas gingivalis and Fusobacterium nucleatum in the oral cavity. This not only severely affects alveolar bone and connective tissues but also has potential associations with distal intestinal in ammation. This study aimed to elucidate the therapeutic effects of Streptococcus cristatus CA119 on experimental periodontitis in rats and its impact on intestinal morphology. The results demonstrate that CA119 can colonize the oral cavity and exerts antagonistic effects on P. gingivalis and F. nucleatum, leading to a signi cant reduction in the oral pathogen load. Following CA119 intervention, a signi cant alleviation in weight loss in rats induced by periodontitis was observed (P < 0.001). CA119 also regulated the expression of IL-6 (P < 0.05), IL-1β (P < 0.001), IL-18 (P < 0.001), COX-2 (P < 0.001), iNOS (P < 0.001), and MCP-1 (P < 0.01) in the gingival tissue. Additionally, CA119 reduced oxidative stress levels in rats and enhanced their antioxidant capacity. Microcomputed tomography (micro-CT) and histological analysis revealed that CA119 signi cantly reduced alveolar bone loss and reversed the down-regulation of OPG/RANK (P < 0.001). Furthermore, CA119 exhibits a signi cant protective effect against intestinal in ammation induced by periodontal disease and improves the colonic morphology in rats. In conclusion, this study demonstrates the role of CA119, a potential oral probiotic, in preventing and treating experimental periodontitis, which underscores the potential of probiotics as a complementary approach to traditional periodontal care.

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Effects of Lactobacillus reuteri MG5346 on Receptor Activator of Nuclear Factor-Kappa B Ligand (RANKL)-Induced Osteoclastogenesis and Ligature-Induced Experimental Periodontitis Rats

Cited by 6 publications

References 43 publications

Curcumin Attenuates Periodontal Injury via Inhibiting Ferroptosis of Ligature-Induced Periodontitis in Mice

Curcumin Attenuates Periodontal Injury via Inhibiting Ferroptosis of Ligature-Induced Periodontitis in Mice

In Vitro Effects of Weissella cibaria CMU and CMS1 on Receptor Activator of NF-κB Ligand (RANKL)-Induced Osteoclast Differentiation

Preventive and Therapeutic Potential of Streptococcus cristatus CA119 in Experimental Periodontitis in Rats

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