Effects of Lactobacillus helveticus Fermentation on the
      Ca2+ Release and Antioxidative Properties of Sheep Bone
     Hydrolysate

Han, Keguang; Cao, Jing; Wang, Jinghui; Chen, Jing; Yuan, Kai; Pang, Fengping; Gu, Shaopeng; Huo, Nairui

doi:10.5851/kosfa.2018.e32

Cited by 9 publications

(13 citation statements)

References 19 publications

(25 reference statements)

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“…Studies have shown that this probiotic lowers serum cholesterol and triglyceride levels [18,19]. Lactobacillus plantarum ST-III promotes intracellular Ca 2 + assimilation at the cellular level to reduce ERS caused by liver injury [20][21][22]. Therefore, in the present study, we investigated the effects of Lactobacillus plantarum ST-III culture supernatants (L-P-cs) in a mice model of CCl 4 -induced liver injury.…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: LP-cs Ameliorated CCl 4 -Induced Acute Liver Injury by Suppression of Cellular Stress

Xu¹,

et al. 2021

Preprint

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Background: Acute liver injury (ALI) involves excessive oxidative stress(OS) and inflammatory responses, leading to a high mortality rate due to lack of effective therapy. Carbon tetrachloride (CCl4) is widely used to induce ALI by induction of reactive oxygen species. Probiotics, including Lactobacillus plantarum ST-III, have been shown to produce antibacterial and antioxidant substances such as organic acids or bacteriocins that reduce liver damage. Nevertheless, the effect of Lactobacillus plantarum ST-III culture supernatant (L-P-cs) on CCl4-induced liver injury remains unclear.Methods: Mice were pretreated with L-P-cs or medium for 14days before one dose of 0.2% CCl4 at 10ml/kg body weight delivered by intraperitoneal injection. CCl4-induced liver injury was examined by measuring serum levels of liver transaminases and high mobility-group box 1 protein (HMGB1) and liver histological staining. Inflammation and apoptosis in liver were evaluated by real-time PCR, enzyme-linked immunosorbent assay (ELISA), and TUNEL staining. Apoptosis in NCTC 1469 cells was detected using CCK8 and western blotting (WB). In liver, OS and endoplasmic reticulum stress(ERS)-related proteins were measured using kits and WB.Results: L-P-cs significantly ameliorated CCl4-induced liver injury and reduced CCl4- induced inflammatory response and apoptosis, consistent with NCTC 1469 cells' results. L-P-cs also restored CCl4-induced increases in cell OS and ERS to normalize liver function. Specifically, L-P-cs pretreatment decreased CCl4-induced increases in nuclear factor (erythroid-2 related) factor 2, HO-1, superoxide dismutase, glucose regulatory protein, and activating transcription factor 6.Conclusion: L-P-cs synergistically improves liver lobule necrosis, hepatocyte inflammation, and apoptosis by reducing liver OS and ERS.

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Section: Introductionmentioning

confidence: 99%

WITHDRAWN: LP-cs Ameliorated CCl 4 -Induced Acute Liver Injury by Suppression of Cellular Stress

Xu¹,

et al. 2021

Preprint

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“…Starch was the least effective in this regard and conferred a DH of only 1.29%. This result was consistent with a previous report indicating that glucose, lactose, and fructose could be effectively metabolized by L. helveticus strains 28 and glucose was the most favorable for supporting their proteolytic activity 29 . The lower effectiveness of starch might be ascribed to the poor amylolytic activity of the strain used.…”

Section: Resultssupporting

confidence: 93%

“…The LPP content in the hydrolysate was positively related to the DH of CGM; that is, the highest LPP content of 369.1 mg kg −1 occurred in 20 g kg −1 glucose and the lowest value of 70.7 mg kg −1 was seen in 50 g kg −1 glucose. Such a great difference between the maximum and minimum LPP content compared with that of DH implied that the glucose concentration could possibly affect the proteolytic enzyme profile of the strain, 29 which affected the release of LPP from CGM; or LPP was preferably consumed by the bacteria in the case of nitrogen starvation caused by high glucose concentrations.…”

Section: Resultsmentioning

confidence: 99%

Release of Leu‐Pro‐Pro from corn gluten meal by fermentation with a Lactobacillus helveticus strain

Zhang

Xiao

et al. 2021

J Sci Food Agric

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BACKGROUND: Angiotensin-converting enzyme (ACE) inhibitory peptides are potential alternatives to the synthetic ACE inhibitory drugs, but the in vivo antihypertensive effects of most of them have not been confirmed. The tripeptide Leu-Pro-Pro (LPP) is one of the few peptides that have been proved clinically effective in reducing the blood pressure of hypertensive patients and casein is currently its major source. LPP is contained in multiple fractions of zein, and corn gluten meal (CGM) is hence a potential new source of LPP. For this purpose, CGM was fermented with a Lactobacillus helveticus strain and the medium composition was optimized; the decoloration of the resultant hydrolysate was investigated as well.RESULTS: LPP could be successfully released from CGM by fermentation with the strain Lactobacillus helveticus CICC 22536. The highest LPP content and protein recovery of 561 mg kg −1 and 14.92% occurred in the medium containing 20 g L −1 glucose, 15 g L −1 beef extract, 60 g L −1 CGM, 10 g L −1 CaCO 3 , 0.5 g L −1 NaCl, and inoculation amount 6%. The supplementation of Fla-vourzyme® further improved the two parameters to 662 mg kg −1 and 36.94%, respectively. The permeate of the hydrolysate after ultrafiltration through a 5 kDa membrane could be effectively decolored by the macroporous resin XAD-16 without notable protein loss, and its LPP content was further boosted to 743 mg kg −1 .CONCLUSION: CGM is a potential new source of LPP and its ultrafiltered and decolored hydrolysate could be used to develop new antihypertensive functional foods.

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“…Additionally, reductones formed during fermentation could react with free radicals to stabilize and terminate radical chain reactions [33]. is could explain different results previously obtained for the antioxidant properties of fermented milk of different sources which greatly depend on several factors including the food matrix composition, overall peptidic profile, amino acid composition of the individual peptide, and the large differences between species or even strains used in fermentation [34].…”

Section: Resultsmentioning

confidence: 99%

Ex Vivo Study of Laban's Role in Decreasing Hemolysis Crisis in G6PD-Deficient Patients

Zam

Belal²

2020

Journal of Nutrition and Metabolism

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In spite of the vast nutritional and environmental benefits provided by fava bean (Vicia faba), the ingestion of vicine/convicine provokes an acute hemolytic anemia called favism in individuals with a glucose-6-phosphate dehydrogenase (G6PD) deficiency. The elimination of these glycosides is a goal that could be accomplished using different processing methods including bacteriological treatment. Laban as a good source of lactic acid bacteria was tested in an ex vivo assay on human blood samples in order to determine its capacity in decreasing the hemolysis crisis induced by the ingestion of fava beans. Results indicate a significant decrease in human blood cell hemolysis after the treatment of fava beans by Laban. This decrease in hemolysis was also correlated with the G6PD deficiency categorization. The highest hemolysis level (mean: 23.11 ± 0.76%) was observed in samples with G6PD activity between 10 and 30%, while the lowest hemolysis level (mean: 5.75 ± 0.64%) was observed in samples with G6PD activity more than 60%. This decrease was correlated with a high antioxidant capacity of Laban (51.61 ± 1.13% expressed by the percentage inhibition of DPPH radical). The counts of isolates from MRS and M17 culture plates were 6.75 ± 0.095 and 7.91 ± 0.061 log cfu ml–1, respectively. In conclusion, the synergy between the antioxidant properties of Laban and the possible decrease of vicine and convicine concentrations by lactobacillus found in the fermented dairy products could explain the ability of Laban to reduce the hemolysis crisis ex vivo.

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Effects of Lactobacillus helveticus Fermentation on the Ca2+ Release and Antioxidative Properties of Sheep Bone Hydrolysate

Cited by 9 publications

References 19 publications

WITHDRAWN: LP-cs Ameliorated CCl 4 -Induced Acute Liver Injury by Suppression of Cellular Stress

WITHDRAWN: LP-cs Ameliorated CCl 4 -Induced Acute Liver Injury by Suppression of Cellular Stress

Release of Leu‐Pro‐Pro from corn gluten meal by fermentation with a Lactobacillus helveticus strain

Ex Vivo Study of Laban's Role in Decreasing Hemolysis Crisis in G6PD-Deficient Patients

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