Effects of Lactobacillus fermentation on Eucheuma spinosum polysaccharides: Characterization and mast cell membrane stabilizing activity

In vivo evaluations of Lactobacillus-fermented Eucheuma spinosum polysaccharides on alleviating food allergy activity

“…After administration of 200 mg kg −1 of F1-ESP-3, the levels of mast cell protease and histamine in the serum of mice decreased to 397.43 pg mL −1 and 142.22 ng mL −1 , respectively, and the difference was highly significant. This result is highly consistent with the results we found in vitro , 2 further indicating that F1-ESP-3 can also inhibit the release of mast cell proteases, histamines and other substances by mast cells in vivo .…”

“…With the rise of probiotic fermentation in the food industry, fermented foods have attracted worldwide attention. 1 In previous studies, we were surprised to find that the molecular weight and viscosity of polysaccharides produced by fermentation of Eucheuma spinosum by screened Lactobacillus were significantly reduced, and the anti-allergic activity in vitro was also significantly improved, 2 which was very consistent with the phenomenon reported by researchers in related fields. 3 Xu et al prepared an acidic polysaccharide extract called RBAPS from burdock residue fermentation by Rhizopus nigricans .…”

“…sakei -fermented Eucheuma spinosum polysaccharides (F1-ESP-3) were prepared and purified as previously reported. 2 Ovalbumin and Imject Alum were obtained from Sigma Aldrich, Inc. (St. Louis, MO, USA). Sodium cromoglycate was provided by Aladdin Biochemical Technology Co., Ltd (Shanghai, China).…”

Marine-Derived Alternariol Monomethyl Ether Alleviates Ovalbumin-Induced Food Allergy by Suppressing MAPK and NF-κB Signaling Pathways of Mast Cells

“…The cells were incubated with 200 μL of solution of fluorescein isothiocyanate (FITC)-labeled phalloidin for 30 min. Changes in F-actin were observed using a fluorescence microscope …”

“…Changes in F-actin were observed using a fluorescence microscope. 26 Transcription Analysis. To conduct transcription analysis, BMMC cells treated with either PBS, DNP-BSA, or AME were collected.…”