Effects of Lactobacillus acidophilus dietary supplementation on the performance, intestinal barrier function, rectal microflora and serum immune function in weaned piglets challenged with Escherichia coli lipopolysaccharide

Abstract

This study was conducted with a lipopolysaccharide (LPS)-challenged piglet model to determine the effects of diets containing Lactobacillus acidophilus on the performance, intestinal barrier function, rectal microflora and serum immune function. A total of 150 piglets (initial body weight (BW) 7.53 ± 0.21 kg) were allotted to one of the following diets, including a basal diet, a basal diet supplemented with 250 mg/kg Flavomycin, or basal diet plus 0.05, 0.1 or 0.2 % L. acidophilus. On day 28 of the trial, the pigs were given an intraperitoneal injection of LPS (200 μg/kg body weight) followed by blood collection 3 h later. Diets with either antibiotics, 0.1 or 0.2 % Lactobacillus increased (P < 0.05) the final BW and decreased (P < 0.05) feed gain ratio (F/G) compared with the control group. Pigs fed diets containing antibiotic or Lactobacillus had greater average daily gain (ADG) (P < 0.05) than pigs fed the control diet. The rectal content Lactobacillus counts for pigs fed diet containing Lactobacillus were significant higher (P < 0.01) than those fed antibiotic or control diet. Feeding the Lactobacillus diets decreased the Escherichia coli counts of rectal content (P < 0.01). Pigs fed diets containing 0.1 or 0.2 % Lactobacillus decreased serum DAO activity (P < 0.05) compared with pigs fed the control diet. Serum IL-10 concentration was enhanced in pigs fed the diet with Lactobacillus compared to pigs fed the control diet and antibiotic diet. Feeding a diet with Lactobacillus reduced (P < 0.05) IFN-γ concentration compared to the control diet. Inclusion of Lactobacillus in diets fed to pigs reduced TNF-α concentration compared with pigs fed no Lactobacillus (P < 0.05). These results indicate that feeding with L. acidophilus improved growth performance and protected against LPS-induced inflammatory status.

Effects of Lactobacillus acidophilus and zinc oxide on the growth performance, jejunal morphology and immune function of weaned piglet following an Escherichia coli K88 challenge

“…In the present study, the results indicated that piglets fed 5 Â 10 10 CFU L. acidophilus or 3000 mg ZnO/kg feed had greater ADG, lower F:G than the unsupplemetend group. Consistently, previous studies have established improved ADFI and ADG of piglets fed Lactobacillus strains (Lee et al 2012;Yang et al 2014;Qiao et al 2015) or ZnO (Hill et al 2001). Thus, we can speculate that both LA and ZnO could improve the growth and might partly attenuate the effects of the stress of piglets during the post-weaning period.…”

“…Moreover, Lactobacillus strains would synthesise exopolysaccharides in their growth phase (Lebeer et al 2008). In our previous study, we have proved that L. acidophilus could modify intestinal microflora (Qiao et al 2015), which may be important to enhance health state of piglets. Previous studies reported that the breakdown of intestinal barrier function was the deleterious effect of weaning (Spreeuwenberg et al 2001;Boudry et al 2004).…”

“…Therefore, the aim of the present study was to evaluate the impact of a new probiotic L. acidophilus (Qiao et al 2015) and ZnO on the growth performance, jejunal morphology and immune function of weaned piglet orally challenged with E. coli K88.…”

Probiotic supplementation can positively affect anxiety and depressive symptoms: a systematic review of randomized controlled trials

“…Lactobacillus rhamnosus JB-1) [17]. In parallel, swine-based models also highlight the critical role of the GI microbiota and bacterial supplementation in enhancing immune development and functioning [18][19][20][21][22][23][24][25][26]. These data include evidence of altered immune development and function in differentially-raised (conventional vs. isolator) newborn [18,19] and GF piglets [20,21].…”

“…Butyrate) and branched chain fatty acids [22], (b) reduced concentrations of Clostridium and increased concentrations of Lactobacillus [22,23], (c) improved immune functioning [22,24], (d) greater antioxidant activity [25], and (e) reduced expression of proteins linked to the stress response [26] following supplementations with Lactobacillus fermentum I5007 [22,25,26], Lactobacillus delbrueckii subsp. bulgaricus 2038 [23], and Lactobacillus acidophilus [24].…”