Effects of Lactiplantibacillus plantarum LPJZ-658 Supplementation on the Production, Meat Quality, Intestinal Morphology, and Cecal Microbiota of Broilers Chickens

Abstract

This study aimed to investigate the effects of L. plantarum LPJZ-658 on the production, meat quality, intestinal morphology, and cecal microbiota of broilers. White-feathered broilers (1 day old, n = 600) were randomly assigned to two groups and raised for six weeks. The individuals in the LPJZ-658 group were supplemented with 2.6 × 109 cfu/g LPJZ-658. The growth performance, meat quality, intestinal epithelium morphology, and cecal microbiota were observed. The results showed that the average daily gain, average daily feed intake, and feed conversion ratio of broilers in the LPJZ-658 group were significantly improved. In addition, the LPJZ-658 groups had a higher thigh muscle (TM) yield, TM color, TMpH24h, breast muscle (BM) pH24h, and BM color24h, while the BM cooking loss was significantly lower than the CON group. Moreover, supplementation with LPJZ-658 increased ileum and cecum length, duodenum and ileum villus height, and ileum villus height/crypt depth ratio. Furthermore, 16S rRNA sequencing revealed the dietary LPJZ-658 supplementation modulated the diversity and composition of cecal microflora. At the phylum level, the relative abundances of Proteobacteria, Actinobacteria, Verrucomicrobiota, and Acidobacteriota were significantly higher. In addition, LPJZ-658 substantially decreased the genus relative abundances of Streptococcus, Veillonella, Neisseria, and Haemophilus compared with the CON group and facilitated the growth and colonization of beneficial cecal bacteria, such as OBacteroides, Phascolarctobacterium, Bacillus, and Akkermansia. It was concluded that LPJZ-658 supplementation significantly increased growth production, improved meat quality and intestinal status, and modulated the intestinal microbiota in the broilers.

The impact of dietary supplementation of polysaccharide derived from Polygonatum sibiricum on growth, antioxidant capacity, meat quality, digestive physiology, and gut microbiota in broiler chickens

“…In addition, we observed an increase in the abundance of Verrucomicrobiota in digesta following PSP supplementation, aligning with previous studies ( Liu et al, 2023a ; Liu et al, 2023b ). Liu et al (2023b) demonstrated that Verrucomicrobiota has the potential to enhance various intestinal aspects, such as VH and VH/CD ratio and the length of ileum and cecum. Another study by Liu et al (2023a) indicated the potential of Verrucomicrobiota in increasing intestinal villus height.…”

Exploring the Regulatory Effect of LPJZ-658 on Copper Deficiency Combined with Sugar-Induced MASLD in Middle-Aged Mice Based on Multi-Omics Analysis

“…LPJZ-658 is a strain of Lactiplantibacillus plantarum isolated by our group. Our practice has confirmed that LPJZ-658 improves lipid metabolism in late-laying laying hens and broiler chickens [39]. It is important to note that we found that LPJZ-658 ameliorated hepatic steatosis, inflammation, and fibrosis in NASH model mice by mediating bile acid metabolism and the gut microbiota [29], which suggests that LPJZ-658 has the potential to improve MASLD.…”

Lactiplantibacillus plantarum LPJZ-658 Improves Non-Alcoholic Steatohepatitis by Modulating Bile Acid Metabolism and Gut Microbiota in Mice

“…In recent years, growing evidence has established the therapeutic function of probiotics in the prevention/treatment of NAFLD [ 30 , 31 ]. In previous studies, we showed that LPJZ-658 supplementation improved the productivity of laying hens and broilers by modulating lipid metabolism and gut microbiota [ 27 ]. In the present study, we evaluated the impact of LPJZ-658 on NASH pathology.…”

“…Notably, we found that LPJZ-658 contains a gene-encoding bile salt hydrolase (BSH), indicating that it may have the ability to regulate bile acid metabolism [ 26 ]. We further documented the effect of LPJZ-658 on improving the production performance and modulating the metabolism of both laying hens and broilers [ 27 ]. In the current study, we found that both LGG and LPJZ-658 were effective in the prevention of NASH, but LPJZ-658 was superior to LGG in liver injury and lipid metabolism.…”