Effects of Lacticaseibacillus rhamnosus GG supplementation, via food and non-food matrices, on children’s health promotion: A scoping review

Xavier-Santos, Douglas; Scharlack, Nayara Kastem; Pena, Fabíola de Lima; Antunes, Adriane Elisabete Costa

doi:10.1016/j.foodres.2022.111518

Cited by 9 publications

(2 citation statements)

References 112 publications

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“…L. rhamnosus , reclassified as Lacticaseibacillus rhamnosus [ 31 ], is commonly found in the mouth and considered a ‘good’ bacterium that breaks down the food contents, absorbs nutrients, and also fights the ‘bad’ bacteria. It is added to fermented foods such as yogurt and is commonly used in probiotic supplements [ 32 ]. Therefore, the present study aimed to microencapsulate L. rhamnosus GG with a single layer of polyelectrolytes, including sodium alginate, calcium chloride, and disodium hydrogen phosphate, to establish a system for the simultaneous oral delivery of probiotics (i.e., L. rhamnosus GG) and drugs (i.e., bovine lactoferrin (bLf)).…”

Section: Introductionmentioning

confidence: 99%

Microencapsulation of Lacticaseibacillus rhamnosus GG for Oral Delivery of Bovine Lactoferrin: Study of Encapsulation Stability, Cell Viability, and Drug Release

et al. 2022

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Probiotics are delivered orally for treating gastrointestinal tract (GIT) infections; thus, they should be protected from the harsh environment of the GIT, such as through microencapsulation. Here, we microencapsulated cells of the probiotic Lacticaseibacillus rhamnosus GG via the liquid-droplet-forming method and evaluated them for oral delivery of bovine lactoferrin (bLf). Briefly, sodium alginate capsules (G-capsules) were first prepared, crosslinked with calcium chloride (C-capsules), and then modified with disodium hydrogen phosphate (M-capsules). All capsules showed good swelling behavior in the order of G-capsules > C-capsules > M-capsules in simulated gastric fluid (SGF, pH 2) and simulated intestinal fluid (SIF, pH 7.2). FE-SEM observations showed the formation of porous surfaces and successful microencapsulation of L. rhamnosus GG cells. The microencapsulated probiotics showed 85% and 77% viability in SGF and SIF, respectively, after 300 min. Compared to the 65% and 70% viability of gelation-encapsulated and crosslinking-encapsulated L. rhamnosus GG cells, respectively, the mineralization-encapsulated cells showed up to 85% viability after 300 min in SIF. The entrapment of bLf in the mineralization-encapsulated L. rhamnosus GG cells did not show any toxicity to the cells. FTIR spectroscopy confirmed the successful surface modification of L. rhamnosus GG cells via gelation, crosslinking, and mineralization, along with the entrapment of bLf on the surface of microencapsulated cells. The findings of these studies show that the microencapsulated L. rhamnosus GG cells with natural polyelectrolytes could be used as stable carriers for the oral and sustainable delivery of beneficial biotherapeutics without compromising their viability and the activity of probiotics.

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Section: Introductionmentioning

confidence: 99%

Microencapsulation of Lacticaseibacillus rhamnosus GG for Oral Delivery of Bovine Lactoferrin: Study of Encapsulation Stability, Cell Viability, and Drug Release

et al. 2022

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“…Accurate identification and quantification of live probiotics are essential to ensure production process control and quality. Lacticaseibacillus rhamnosus , as one of the most popular Lactobacillus strains, has been widely studied because of its safety profile and desirable features of conventional probiotics ( Kalliomäki et al, 2001 ; Mathipa-Mdakane and Thantsha, 2022 ; Xavier-Santos et al, 2022 ). L. rhamnosus species, e.g., LGG, HN001 etc., possess great market value in food industry attributed to their excellent fermentation performance and probiotic effect.…”

Section: Introductionmentioning

confidence: 99%

Identification and quantification of viable Lacticaseibacillus rhamnosus in probiotics using validated PMA-qPCR method

Guo,

Ze,

Feng

et al. 2024

Front. Microbiol.

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The identification and quantification of viable bacteria at the species/strain level in compound probiotic products is challenging now. Molecular biology methods, e.g., propidium monoazide (PMA) combination with qPCR, have gained prominence for targeted viable cell counts. This study endeavors to establish a robust PMA-qPCR method for viable Lacticaseibacillus rhamnosus detection and systematically validated key metrics encompassing relative trueness, accuracy, limit of quantification, linear, and range. The inclusivity and exclusivity notably underscored high specificity of the primers for L. rhamnosus, which allowed accurate identification of the target bacteria. Furthermore, the conditions employed for PMA treatment were fully verified by 24 different L. rhamnosus including type strain, commercial strains, etc., confirming its effective discrimination between live and dead bacteria. A standard curve constructed by type strain could apply to commercial strains to convert qPCR Cq values to viable cell numbers. The established PMA-qPCR method was applied to 46 samples including pure cultures, probiotics as food ingredients, and compound probiotic products. Noteworthy is the congruity observed between measured and theoretical values within a 95% confidence interval of the upper and lower limits of agreement, demonstrating the relative trueness of this method. Moreover, accurate results were obtained when viable L. rhamnosus ranging from 103 to 108 CFU/mL. The comprehensive appraisal of PMA-qPCR performances provides potential industrial applications of this new technology in quality control and supervision of probiotic products.

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What We Know About the Actual Role of Traditional Probiotics in Health and Disease

Al-Akayleh,

Agha,

Al‐Remawi

et al. 2024

Probiotics & Antimicro. Prot.

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Effects of Lacticaseibacillus rhamnosus GG supplementation, via food and non-food matrices, on children’s health promotion: A scoping review

Cited by 9 publications

References 112 publications

Microencapsulation of Lacticaseibacillus rhamnosus GG for Oral Delivery of Bovine Lactoferrin: Study of Encapsulation Stability, Cell Viability, and Drug Release

Microencapsulation of Lacticaseibacillus rhamnosus GG for Oral Delivery of Bovine Lactoferrin: Study of Encapsulation Stability, Cell Viability, and Drug Release

Identification and quantification of viable Lacticaseibacillus rhamnosus in probiotics using validated PMA-qPCR method

What We Know About the Actual Role of Traditional Probiotics in Health and Disease

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