Effects of l-Proline and Cold Treatment on Pepper (Capsicum annuum L.) Anther Culture

Özkum, Dudu; Tıpırdamaz, Rukiye

doi:10.1007/978-3-540-95991-5_14

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…Total phenolic content was determined according to Koç et al (2010) with modifications. The fresh leaf and root samples (0.1 g) were frozen in liquid nitrogen and stored at -80°C.…”

Section: Methodsmentioning

confidence: 99%

Pepper Rootstock and Scion Physiological Responses Under Drought Stress

et al. 2019

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In vegetables, tolerance to drought can be improved by grafting commercial varieties onto drought tolerant rootstocks. Grafting has emerged as a tool that copes with drought stress. In previous results, the A25 pepper rootstock accession showed good tolerance to drought in fruit production terms compared with non-grafted plants and other rootstocks. The aim of this work was to study if short-term exposure to drought in grafted plants using A25 as a rootstock would show tolerance to drought now. To fulfill this objective, some physiological processes involved in roots (rootstock) and leaves (scion) of grafted pepper plants were analyzed. Pepper plants not grafted (A), self-grafted (A/A), and grafted onto a tolerant pepper rootstock A25 (A/A25) were grown under severe water stress induced by PEG addition (-0.55 MPa) or under control conditions for 7 days in hydroponic pure solution. According to our results, water stress severity was alleviated by using the A25 rootstock in grafted plants (A/A25), which indicated that mechanisms stimulated by roots are essential to withstand stress. A/A25 had a bigger root biomass compared with plants A and A/A that resulted in better water absorption, water retention capacity and a sustained CO2 assimilation rate. Consequently, plants A/A25 had a better carbon balance, supported by greater nitrate reductase activity located mainly in leaves. In the non-grafted and self-grafted plants, the photosynthesis rate lowered due to stomatal closure, which limited transpiration. Consequently, part of NO3- uptake was reduced in roots. This condition limited water uptake and CO2 fixation in plants A and A/A under drought stress, and accelerated oxidative damage by producing reactive oxygen species (ROS) and H2O2, which were highest in their leaves, indicating great sensitivity to drought stress and induced membrane lipid peroxidation. However, drought deleterious effects were slightly marked in plants A compared to A/A. To conclude, the A25 rootstock protects the scion against oxidative stress, which is provoked by drought, and shows better C and N balances that enabled the biomass to be maintained under water stress for short-term exposure, with higher yields in the field.

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“…Total phenolic content was determined according to Koç et al (2010) with modifications. The fresh leaf and root samples (0.1 g) were frozen in liquid nitrogen and stored at -80°C.…”

Section: Methodsmentioning

confidence: 99%

Pepper Rootstock and Scion Physiological Responses Under Drought Stress

et al. 2019

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“…The use of different mixtures of plant growth regulators had been additionally stated with unique success (Taskin et al, 2011;Irikova et al, 2011). It was found by Qin and Rotino (1993), that for promoting embryogenesis in some recalcitrant pepper genotypes the combination of BA(0.6 mg L -1 ) with 2.4-D is used, while Ozkum and Tipirdamaz (2011) stated that NAA(4.0 mg l -1 ) combined with BA(1.0 mg l -1 ) initiate microspore embryogenesis. To sum up, androgenesis is initiated in growth regulator-free medium (Kim et al, 2008) just as in media upgraded with 5 µM indole-3-acetic acid (IAA) and 2.5 µM zeatin (Supena et al, 2006(Supena et al, , 2011, or in the media of 0.5 mg•l −1 kinetin (Kin) and 0.5 mg•l −1 2,4dichlorophenoxyacetic acid (2,4-D) stated by Lantos et al (2009).…”

Section: Medium Composition and Growth Regulatorsmentioning

confidence: 99%

Doubled Haploid Production in Capsicum Annuum L. Using Anther Culture : A Review

Jha¹,

Choudhary²,

Agarwal³

2021

PLANT ARCHIVES

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Capsicum spp. is a horticulture crop with great potential and is supposed to be the fourth most significant vegetable in the earth. Capsicum genus covers several dozens of species which contain vast range of nutritional and medicinal properties. The process of inbreed development for hybrid variety production is fastened by the use of doubled haploid (DH) system. Doubled haploid (DH) system plays an important role in breeding programs and developmental studies. The production of haploid and doubled haploid plants using the anther cultures of the desired genotype is one of opportunities for rapid stabilization of new variants. There is an ongoing research to develop and improve the effectiveness of these methods by looking at the factors involved in different phases like induction and regeneration. Factors predominantly incorporate condition of donor plants, plant genotypes, medium components, cultured gametophytic cells temperature treatments of (cold pre-treatment, heat shock), the developmental period of early on gametophytic cells and use of various segments like Charcoal and plant development regulators. Stress treatment is one of the conditions which are utilized for the incitement of gametophytic cells to modify toward the sporophytic pathway.

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“…Total phenolic content was determined according to Koç et al (2010) with modifications. Fresh leaf samples (0.1 g) were frozen in liquid nitrogen and stored at -80°C.…”

Section: Total Phenolic Contentmentioning

confidence: 99%

Physiological characterization of a pepper hybrid rootstock designed to cope with salinity stress

López-Serrano

Canet-Sanchis

Selak

et al. 2020

Plant Physiology and Biochemistry

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Effects of l-Proline and Cold Treatment on Pepper (Capsicum annuum L.) Anther Culture

Cited by 3 publications

References 19 publications

Pepper Rootstock and Scion Physiological Responses Under Drought Stress

Pepper Rootstock and Scion Physiological Responses Under Drought Stress

Doubled Haploid Production in Capsicum Annuum L. Using Anther Culture : A Review

Physiological characterization of a pepper hybrid rootstock designed to cope with salinity stress

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