Effects of L. plantarum HY7715 on the Gut Microbial Community and Riboflavin Production in a Three-Stage Semi-Continuous Simulated Gut System

Abstract

Probiotics should be well established in the gut, passing through the digestive tract with a high degree of viability, and produce metabolites that improve the gut environment by interacting with the gut microbiome. Our previous study revealed that the Lactiplantibacillus plantarum HY7715 strain shows good bile acid resistance and a riboflavin production capacity. To confirm the interaction between HY7715 and gut microbiome, we performed a metabolite and microbiome study using a simulated gut system (SGS) that mimics the intestinal environment. Changes in the microbiome were confirmed and compared with L. plantarum NCDO1752 as the control. After 14 days, the HY7715 treatment group showed a relatively high butyrate content compared to the control group, which showed increased acetate and propionate concentrations. Moreover, the riboflavin content was higher in the HY7715 treatment group, whereas the NCDO1752 treatment group produced only small amounts of riboflavin during the treatment period and showed a tendency to decrease during the washout stage; however, the HY7715 group produced riboflavin continuously in the ascending colon during the washout period. A correlation analysis of the genus that increased as the content of riboflavin increased revealed butyrate-producing microorganisms, such as Blautia and Flavonifractor. In conclusion, treatment with L. plantarum HY7715 induced the production and maintenance of riboflavin and the enrichment of the intestinal microbiome

Effects of black soldier fly larvae as protein or fat sources on apparent nutrient digestibility, fecal microbiota, and metabolic profiles in beagle dogs

“…Among them, glutathione metabolism and nicotinate and nicotinamide metabolism participate in antioxidant activity (Seo et al, 2018) and protect against aging (Si et al, 2019), respectively, which may benefit gut health in dogs. Moreover, 20% DBP increased the relative abundances of Flavonifractor, Blautia, and Enterococcus, which inhibited the secretion of acetoacetate CoA-transferase, thereby reducing the production of butyrate (Kellingray et al, 2018;Hong et al, 2021). This further verified our conclusion.…”

“…Moreover, Fusobacterium, Faecalibacterium, Collinsella, and Bacteroides were the dominant bacterial genera among the three groups and showed no difference between the CON and DBP or BF groups. Upon further analysis of bacterial genera, we found that 20% DBP decreased the relative abundance of SCFA-producing bacteria, including Prevotella_9 (Li et al, 2022b), Lachnospiraceae_NC2004_group (Egerton et al, 2022), Catenibacterium , Allisonella (Zhao et al, 2021), Turicibacter, and Romboutsia (Li et al, 2021), and a previous confirmed that Flavonifractor (Hong et al, 2021), Blautia, and Enterococcus (Kellingray et al, 2018) acted as inhibitors of butyrate, resulting in a reduction in butyrate in feces, thereby increasing fecal pH. SCFAs are produced by the microbial fermentation of undigestible carbohydrates (Kawauchi et al, 2011).…”

Spontaneous Riboflavin-Overproducing Limosilactobacillus reuteri for Biofortification of Fermented Foods

“…Microbiome analysis within the colon stage of the GIDM suggested that the fecal microbiome composition was influenced by time rather than the addition of L. reuteri AMBV339. While the fecal microbial composition did not change, further metabolomic and transcriptomic analyses are encouraged to investigate whether the microbial metabolism was affected by the increased production capacity of riboflavin in the colon after addition of L. reuteri AMBV339, such as shown by Hong et al for L. plantarum HY7715 ( 66 ). However, gut microbiome modulation is not necessarily a prerequisite for beneficial effects of administered lactobacilli and their metabolic products ( 67 ), especially not in healthy donors.…”

Can we microbe-manage our vitamin acquisition for better health?

“…Most probiotics contain species of the Bifidobacterium , Lactobacillus , or Saccharomyces genera, all capable of de novo synthesis of vitamins [ 30 ]. The elevated vitamin production of specific strains could be a novel promising application of probiotics as it can be helpful for both the host and other microbial species as the vitamin can be synthesized and delivered directly into the intestine [ 31 , 32 ]. This would be especially useful for water-soluble vitamins such as B vitamins, which cannot be stored in the body and consequently require a constant supply.…”