Effects of L-carnitine on embryo development of vitrified swamp buffalo oocytes following in vitro fertilization

Liang, Yuanyuan; Yoisungnern, Ton; Huang, Yan-Ni

doi:10.1016/j.livsci.2020.103933

Cited by 4 publications

(6 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Concerning the concentration of L-carnitine in the current work, the blastocyst rates were significantly (p<0.05) higher in both the 0.5 and 1 mM groups than in the other groups. Recently, Liang et al [26] reported that buffalo oocytes treated with 0.6 mg/mL of L-carnitine during in vitro maturation had significantly higher rates of blastocyst formation than controls. Similar to our results, Khanmohammadi et al [38] found that the developmental rate of 1-cell embryos to the blastocyst stage and the expansion of mouse blastocysts were observed in a medium supplemented with 0.5 mM of acetyl L-carnitine.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, L-carnitine has been shown to respond to lipid droplets distributed from the peripheral area to the inner cytoplasm in oocytes [19]. In buffalo, few studies have reported the supplementation of L-carnitine to in vitro maturation and embryo culture media [24][25][26]. However, the best dosage and the impact on embryo development and cryotolerance were controversial.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of L-carnitine supplementation on the in vitro developmental competence and cryotolerance of buffalo embryos

et al. 2021

View full text Add to dashboard Cite

Background and Aim: Despite many trials, buffalo embryos have poor cryosurvivability because of their high lipid content. L-carnitine was found to be a lipid-reducing agent when added to oocyte and embryo culture media. The study aimed to determine the most effective concentration of L-carnitine to improve the oocyte developmental competence and cryotolerance of buffalo embryos. Materials and Methods: In vitro maturation and embryo culture media were supplemented with four concentrations of L-carnitine: 0 (control), 0.25, 0.5, and 1 mM. Good-quality embryos on 7 days were vitrified using mixtures of dimethyl sulfoxide and ethylene glycol at two concentrations (3.5 and 7 M). Results: The result showed that the cleavage and morula rates were significantly (p<0.05) higher in the 0.5 mM group. Blastocyst rates were significantly (p<0.05) higher at both 0.5 and 1 mM. The rates of viable embryos directly after thawing were significantly (p<0.05) increased in the 0.5 mM group. No significant difference was found in embryos cultured for 24 h after warming among all the groups. Conclusion: The addition of L-carnitine at a concentration of 0.5 mM to the culture media improves the oocyte developmental competence and cryotolerance of buffalo embryos directly after warming but not after 24 h of culture. Nevertheless, further studies must identify how L-carnitine exerts its beneficial micromechanisms.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of L-carnitine supplementation on the in vitro developmental competence and cryotolerance of buffalo embryos

et al. 2021

View full text Add to dashboard Cite

show abstract

“…It is beneficial in the preservation and conservation of endangered buffalo species using frozen–thawed sperm or oocytes. With ICSI, reproduction of males with motility and fertilizing capacity problems as observed in sex‐sorted sperm cells and some sperm cells after cryopreservation can be made possible as it can improve pronuclear formation and cleavage rate (Liang, Ye, et al, 2011 ) compared with IVF (Liang et al, 2020 ). IVF with sex‐sorted sperm that resulted in poor embryo development can be improved via ICSI.…”

Section: Intracytoplasmic Sperm Injection (Icsi)mentioning

confidence: 99%

“…The low development rate after warming has been attributed to the high lipid content in buffalo oocytes, cytoskeleton damage during freezing, and plasma membrane enriched with cholesterol or unsaturated fatty acids, making it more sensitive to chilling injuries. Most recently, supplementation with 0.6 mg/ml L‐carnitine during IVM improves the buffalo oocytes' survival, IVF rates, and subsequent embryo development, which had been associated with improved mitochondrial activity, enhanced β‐oxidation, and reduced levels of reactive oxygen species (Liang et al, 2020 ). Finally, the addition of cytoskeleton stabilizers, such as CB has been demonstr5ated to reduce injury to oocytes during vitrification.…”

Section: Cryopreservation Of Oocytes and Embryosmentioning

confidence: 99%

Current status of assisted reproductive technologies in buffaloes

Srirattana

Hufana-Durán

Atabay

et al. 2022

Animal Science Journal

Self Cite

View full text Add to dashboard Cite

Buffaloes are raised by small farm holders primarily as source of draft power owing to its resistance to hot climate, disease, and stress conditions. Over the years, transformation of these animals from draft to dairy was deliberately carried out through genetic improvement program leading to the development of buffalo-based enterprises. Buffalo production is now getting more attention and interest from buffalo raisers due to its socioeconomic impact as well as its contribution to propelling the livestock industry in many developing countries. Reproduction of buffaloes, however, is confronted with huge challenge and concern as being generally less efficient to reproduce compared with cattle due to both intrinsic and extrinsic factors such as poor estrus manifestation, silent heat, marked seasonal infertility, postpartum anestrus, long calving interval, delayed puberty, inherently low number of primordial follicles in their ovaries, high incidence of atresia, and apoptosis. Assisted reproductive technologies (ARTs) are major interventions for the efficient utilization of follicle reserve in buffaloes. The present review focuses on estrus and ovulation synchronization for fixed time artificial insemination, in vitro embryo production, intracytoplasmic sperm injection, cryopreservation of oocytes and embryos, somatic cell nuclear transfer, the factors affecting utilization in various ARTs, and future perspectives in buffaloes.

show abstract

“…A pesar de la importancia de las alpacas para el Perú, su reproducción se realiza principalmente por monta natural, sin considerar la variabilidad genética y el mejoramiento de la especie con fines comerciales, lo que ha llevado a una disminución de la calidad genética (Huanca, 2012). Así, la reproducción in vitro se muestra como una alternativa viable para el mejoramiento genético y el aumento de la productividad de la alpaca, ya que esta técnica está altamente desarrollada en bufalinos, bovinos, ovinos y porcinos (Liang y col., 2020;Javvaji y col., 2020;Dubeibe y col., 2019;Gonella Diaza y col., 2013;Rodrigues y col., 2013). Asimismo, se ha reportado la factibilidad de la reproducción de camélidos por inseminación artificial, transferencia de embriones y fertilización in vitro (Ruiz, 2018;Pérez, Zevallos y Perez, 2017;Pacheco, Vélez y Pezo, 2016), siendo necesario el estudio de las condiciones que permitan el desarrollo exitoso de la reproducción de alpacas mediante técnicas in vitro, de suma importancia para la región andina, ya que no existen protocolos específicos para ello.…”

Section: Introductionunclassified

Efecto de factores embriotróficos a diferentes tensiones de oxígeno en cultivo in vitro sobre el desarrollo embrionario de alpacas hasta la etapa de blastocisto

Mamani¹

2022

lgr

View full text Add to dashboard Cite

La alpaca es el camélido sudamericano doméstico de mayor importancia para Perú, país que cuenta con el 87% de la población a nivel regional. Las formas tradicionales de reproducción, no garantizan la calidad genética de los animales, por lo que la reproducción in vitro es una alternativa para su mejoramiento. El objetivo de la investigación fue analizar cómo influyen factores embriotróficos y tensiones de oxígeno en el desarrollo in vitro de ovocitos de alpaca hasta etapa de blastocistos. Se recolectaron ovarios de animales sacrificados y los ovocitos madurados en medio TCM-199, piruvato de sodio, glutamina, estradiol, hormona folículo estimulante, hormona luteinizante, factor de crecimiento epidermal, suero fetal bovino y gentamicina, por 32 horas a 38.5 ºC, al 5% CO2 y humedad relativa mayor de 95%, se fecundaron con semen fresco y cultivado en medio KSOMaa durante 48 h. Se utilizaron dos factores embriotróficos (EGF y IGF-I), dos tensiones de O2 (5 y 20%), más un grupo control sin factor embriotrófico y tres variables respuesta (ovocitos, divisiones y blastocistos). Se aplicó la técnica estadística de análisis de conglomerados para establecer diferencias entre los tratamientos con ? = 0,05. Se observaron diferencias significativas para cada variable respuesta, con máxima producción de divisiones de 24,88% para EGF a 6% O2 y blastocistos 18,4% para IGF-I a 6% O2. Se concluye que la adición de los factores embriotróficos a los medios de cultivo embrionario in vitro y una baja tensión de oxígeno 6% son favorables para el desarrollo embrionario en alpacas.

show abstract

Effects of L-carnitine on embryo development of vitrified swamp buffalo oocytes following in vitro fertilization

Cited by 4 publications

References 39 publications

Impact of L-carnitine supplementation on the in vitro developmental competence and cryotolerance of buffalo embryos

Impact of L-carnitine supplementation on the in vitro developmental competence and cryotolerance of buffalo embryos

Current status of assisted reproductive technologies in buffaloes

Efecto de factores embriotróficos a diferentes tensiones de oxígeno en cultivo in vitro sobre el desarrollo embrionario de alpacas hasta la etapa de blastocisto

Contact Info

Product

Resources

About