Effects of L-Arginine supplementation on semen quality, testosterone concentration and testes histological parameters of Ross 308 breeder roosters

Ahangar, M; Asadzadeh, S; Rezaeipour, Vahid; Shahneh, AZ

doi:10.12980/apjr.6.20170307

Cited by 12 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Administration of nitrate and insulin in diabetic rats could well increase testosterone levels. Our results are in agreement with previous findings demonstrating dietary l ‐arginine at a dose of 2.33 g kg −1 increased the serum level of testosterone in roosters (Ahangar, Asadzadeh, Rezaeipour, & Shahneh, ). However, another study has shown that sodium nitrite (80 mg kg −1 day −1 ) for 12 weeks caused a decrease in serum testosterone level (Alyoussef AI‐Gayyar, ).…”

Section: Discussionsupporting

confidence: 93%

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Oghbaei

Alipour

Hamidian

et al. 2018

Experimental Physiology

View full text Add to dashboard Cite

Inorganic nitrate supplementation is a possible therapeutic agent in diabetes. The aim of this study was to evaluate the effect of nitrate on the reproductive system in streptozotocin-induced diabetic male rats. Fifty male Wistar rats were allocated randomly to five groups: control (C), control plus nitrate (CN), diabetic (D), diabetic plus insulin (DI) and diabetic plus nitrate (DN). Sodium nitrate was administered for 2 months in the drinking water (100 mg l ) of the CN and DN groups. Insulin was injected at 2-4 U daily in the DI group. Serum glucose level and body weight were measured at the beginning of the study and at regular intervals. At the end of the study, serum levels of glucose, insulin, nitrogen oxides (NOx), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone were assessed as well as sperm parameters, testis morphometry and histology, and testicular miR-34b and p53 mRNA expression. Nitrate treatment in diabetic rats significantly improved sperm parameters, epididymal weight, spermatogenesis and testicular histology as well as decreasing serum glucose and testicular p53 gene and miR-34b expression, although it had no effect on serum LH and FSH levels. In diabetic rats, serum insulin and NOx, body weight, testicular and epididymal weight, sperm count and motility, testis morphology, spermatogenesis indices, Johnsen's score, and testosterone were significantly lower than in controls. Nitrate administration increased serum insulin, NOx and testosterone levels in the DN group. Consuming water supplemented with sodium nitrate could improve diabetes-induced testicular functional and structural disorders; these favourable effects may be related to increased serum insulin and decreased serum glucose, as well as modulation of apoptosis in testis.

show abstract

Section: Discussionsupporting

confidence: 93%

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Oghbaei

Alipour

Hamidian

et al. 2018

Experimental Physiology

View full text Add to dashboard Cite

show abstract

“…Ahangar et al. (2017) showed that feeding L ‐arginine to cocks significantly increased testicular weight, sperm concentration, the number of rectilinear motile sperm and plasma T levels. In this experiment, after L ‐Cit supplementation to rams, a significant increase in sperm concentration, viability and the number of linear sperm and a decrease in the number of dead sperms was observed, indicating that L ‐Cit can improve ram semen quality, which may be associated with the metabolism of L ‐Cit through arginine and NO in the body (Cherney et al., 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Ahangar et al. (2017) revealed that supplementing cock feed with L ‐arginine significantly increased testicular weight, sperm concentration, the number of rectilinear motile sperm, and plasma testosterone (T) level. Gilbreath et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of L‐citrulline supplementation on sperm characteristics and hormonal and antioxidant levels in blood and seminal plasma of rams

Zhao

Song

et al. 2022

Reprod Domestic Animals

View full text Add to dashboard Cite

With the aim of providing a theoretical basis for the application of L‐citrulline (L‐Cit) in animal husbandry, the effects of L‐Cit on reproductive hormone levels, antioxidant capacity and semen quality of rams were studied by feeding them varying doses of L‐Cit. A total of 32 rams were randomly divided into four groups with eight rams each. After all rams were trained to donate sperm normally, the control group was fed a basic diet, whereas the experimental groups I, II and III were provided with feed supplemented with 4, 8 and 12 g/d of L‐Cit respectively. The experiment was conducted for 70 days, during which blood samples were collected from the jugular vein on days 0, 15, 30, 45 and 60, and semen samples were collected on days 0, 20, 40 and 60. In the same group, 100 µl of semen was used to test for quality, The rest of the semen sample and blood samples were centrifuged at 600 g for 15 min, and the supernatant and serum, respectively, were used to determine the levels reproductive hormones and antioxidant indices. Ram semen samples were also collected on day 70 and used to study sperm plasma membrane, substitution and mitochondrial membrane potential. Compared with the control group, the groups receiving L‐Cit showed an increase in sperm concentration and number of linear motile sperm (p < .01); a decrease in the number of dead sperm (p < .01); an increase in sperm viability, particularly in groups II and III (p < .01); and an increase in sperm mitochondrial membrane potential (p < .01). Moreover, groups I, II and III showed significantly higher levels of serum gonadotropin‐releasing hormone (GnRH), glutathione peroxidase (GSH‐Px) and nitric oxide (NO) (p < .01). Luteinizing hormone (LH) and follicle‐stimulating hormone (FSH) levels increased in groups I (p < .05), II (p < .05) and III (p < .01), whereas testosterone (T), catalase (CAT) and superoxide dismutase (SOD) levels increased in groups I and II (p < .01). Serum total antioxidant capacity (T‐A) increased (p < .05), whereas both hydroxyl radical (·OH) and peroxy radical (O2·‐) levels decreased (p < .01). Compared with the control, all groups had significantly higher SOD and GSH‐Px in their seminal plasma (p < .01), and groups I, II (p < .05 for both) and III (p < .01) had higher levels of GnRH and FSH. LH, CAT and NO levels increased in group I (p < .05), II and III (p < .01 for both); malondialdehyde levels decreased in groups I, II (p < .05 for both) and group III (p < .01); and O2·‐ levels decreased in groups I, II and III (p < .01). Under our experimental conditions, GnRH, FSH, LH, T, CAT, SOD, T‐A, GSH‐PX and NO levels in the serum and seminal plasma of rams receiving L‐Cit increased, whereas Oestradiol (E2), O2·‐ and ·OH levels in the seminal plasma decreased; this improved the semen quality of rams supplemented with L‐Cit. Moreover, supplementation with 12 g/d gave the best results.

show abstract

“…However, the birds fed diet supplemented with 2.33 g/kg L-Arg had greater seminiferous tubules diameter, sertoli and spermatogonia cell counts than other groups (P<0.05). According to the results of this experiment, it is concluded that dietary L-Arg had positive effects on reproductive traits in roosters [22].…”

Section: Introductionmentioning

confidence: 71%

Ameliorative Effect of L-Arginine as Antioxidant Agent on Hematological and Testicular Functions in Lead-Induced Toxicity in Male Rats

Ibrahuim

2018

AJRB

View full text Add to dashboard Cite

Aims: The present study aimed to elucidate the protective role of L-Arginine on lead – induced suppression of the pathway of heme synthesis and the toxicity of lead on testis. Methodology: The current study included two experiments; the first was carried out on two groups to follow up the changes that could occur in iron status and reproductive system of male rats because of the lead exposure. The second experiment, three comparisons were made between three groups of rats with lead treatment only and other three groups rats with lead treatment followed by additional of daily injection of L-Arginine intraperitoneal about 500 mg L-Arginine / kg.b.wt. / day for 10, 20 and 30 days after the end of lead treatment. Results: Lead significantly abolished heme synthesis presented by decrease in Hb, Fe and ceruloplasmin (Cp) and elevation in total iron binding capacity (TIBC) and serum ferritin. Furthermore, testosterone (total and free) significantly decreased after rats treated with 1% lead acetate while, estradiol (E2) and acid phosphatase (total and prostatic) were elevated. The concentration of dehydroepiandrosterone sulphat (DHEA-S) did not change after lead treatment. Conclusion: Daily treatment with 500 mg L-Arginine/kg body weight prevented the suppressive effects of lead on hematological parameters and testicular functions. The mechanism of L-Arginine action on lead induced changes was attributed to protection of antioxidant capacity in cells in addition to the ability of L-Arginine to scavenge free radicals and acts as cell membrane stabilizer.

show abstract

Effects of L-Arginine supplementation on semen quality, testosterone concentration and testes histological parameters of Ross 308 breeder roosters

Cited by 12 publications

References 14 publications

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Two months sodium nitrate supplementation alleviates testicular injury in streptozotocin‐induced diabetic male rats

Effect of L‐citrulline supplementation on sperm characteristics and hormonal and antioxidant levels in blood and seminal plasma of rams

Ameliorative Effect of L-Arginine as Antioxidant Agent on Hematological and Testicular Functions in Lead-Induced Toxicity in Male Rats

Contact Info

Product

Resources

About