Effects of KIF2A on the prognosis of nasopharyngeal carcinoma and nasopharyngeal carcinoma cells

Abstract

Nasopharyngeal carcinoma (NPC) is a common tumor in south China. Kinesin family member 2A (KIF2A) belongs to the kinesin-13 family and is associated with the growth and invasion of a number of different types of human cancer, including ovarian, breast and prostate cancer. The aim of the present study was to evaluate the expression of KIF2A in NPC and explore the relationship between KIF2A and the basic characteristics of 5-8F cells. Immunohistochemistry was performed on tissues from 97 patients with NPC to assess KIF2A protein expression. KIF2A was knocked down by a specific short interfering (si)RNA in 5-8F cell lines. Cell proliferation, apoptosis and cycle were analyzed by MTT assay and flow cytometry. The invasive ability and angiogenesis were evaluated by Matrigel assay and reverse transcription-quantitative PCR. The level of KIF2A was associated with the growth and migration of primary tumor, nodal status and tumor stage. The viability of KIF2A-knockdown cells was decreased compared with that of the control cells. The number of apoptotic cells, as well as the percentage of cells in the G0/G1 phase, was higher in the KIF2A siRNA group compared with the control group. The invasive and angiogenetic ability of 5-8F cells in the KIF2A siRNA group was decreased compared with the control group. In conclusion, the expression of KIF2A correlated with the poor clinicopathological features in NPC. Therefore, KIF2A may serve an important role in the progression of NPC and proliferation of 5-8F cells, which might present a potential therapeutic target for patients with NPC.

Modeling Neurodevelopmental Disorders and Epilepsy Caused by Loss of Function ofkif2ain Zebrafish

“…Programmed cell death was shown to be increased in many mouse models of microcephaly ( Poulton et al, 2011 ; Little and Dwyer, 2019 ), and, in contrast, a reduction of apoptosis leads to increased brain size ( Kuida et al, 1998 ). Additionally, previous studies have already reported the correlation between KIF2A inactivation and induced apoptosis in cancer cells ( Zhang et al, 2016 , 2019 ; Li et al, 2019 ). The silencing of KIF2A by siRNA resulted in a decrease of PI3 kinase/Akt expression and downstream proteins, a key signaling pathway involved in the regulation of cell proliferation and growth, and ultimately in the initiation of apoptosis ( Wang et al, 2014 ; Zhang et al, 2020 ).…”

Circular RNA circ_IRAK3 contributes to tumor growth through upregulating KIF2A via adsorbing miR-603 in breast cancer

“…KIF2A exerts a key role in the regulation of microtubule dynamics in the process of mitosis [ 41 , 42 ]. Researchers had proved that KIF2A served as an oncogene in a series of tumors, such as nasopharyngeal carcinoma [ 22 ], lung adenocarcinoma [ 43 ], gastric cancer [ 44 ], and glioma [ 45 ]. Wang et al disclosed that KIF2A was associated with poor prognosis of BC patients, and KIF2A silencing curbed migration and proliferation of BC cells [ 32 ].…”

“…KIF2A, a member of the kinesin-13 family, belongs to M-type nonmotile microtubule depolymerase [ 20 ]. KIF2A has been reported to be upregulated in many tumor tissues [ 21 , 22 ]. In addition, we discovered that KIF2A may be a target of miR-603 using the miRDB database ( http://mirdb.org/ ).…”

Aberrant kinesin family member 2A signifies tumor size and invasion, and may help predict prognosis of patients with papillary thyroid carcinoma

“…For instance, KIF2A promotes malignant behaviors of oral squamous carcinoma cells via activating the PI3K/AKT signaling pathway ( 13 ). Furthermore, KIF2A induces cell proliferation and invasion, while suppressing cell apoptosis in nasopharyngeal carcinoma ( 14 ). Within clinical practice, KIF2A exhibits key prognostic value for the management of gastric cancer, colorectal cancer and nasopharyngeal carcinoma ( 15 – 17 ).…”