Effects of Jacalin- a galactose binding lectin on MDA-MB-468, a triple-negative breast cancer cell line, and its combinatorial effect with taxol

Abstract

Plant lectins have been well established to show the affect on the proliferation in different human cancer cells. The anti-proliferative potential of lectins have been ubiquitously investigated from diverse sources and, the basic mechanism has been explored in an elaborated index. In our study, we observed anti-proliferative efficacy of Jacalin (a purified lectin from jackfruit (Artocarpus integrifolia) seeds in MDA-MB-468, a triple-negative breast cancer cell line. This effect was explored in a dose dependent manner. The cells were treated with Jacalin at different time points (6hr and 12hr) and thereafter the recovery in cell propagation was also observed after which the jacalin media was aspirated. Thus, Jacalin was considered as to possess reversible effects on MDA-MB-468 cells. On the other side, Jacalin did not pose the effect in proliferation cascade in PBMCs, taken as primary cell line control. Galactose is well recognized ligands of Jacalin and its pre-incubation could neutralize the effect. Furthermore, MDA-MB-468 cells were also treated with Jacalin in combination with increasing concentration of taxol, keeping jacalin concentration constant (40µg/ml). Taxol at the concentration of 930 nM in combination with jacalin achieved the similar kind of anti-proliferative effect that was observed with 30 µM of taxol. Thus the taxol concentration could be reduced if given in combination of jacalin, preventing the side effect of taxol due to higher dose.

Jacalin-Curcumin Complex Sensitizes the Breast Cancer MDA-MB-231 Cell Line

“…The combined treatment of jacalin (40 µg/mL) with the established anti-cancer drug taxol (50 µM) reduced cell growth of the triple-negative MDA-MB-468 cell line to 50% after 48 h. To reduce the high-dose treatment and potential side effects, taxol is combined with the natural compound jacalin [50]. Similar to this, our study shows that jacalin-curcumin treatment causes a significant inhibition of cell growth of 50% after 48 h, using a two-times-lower concentration of curcumin (25 µM) than taxol (50 µM).…”

Finding out various potentials and possibilities of jackfruit seed and its usage in the industry: a review

“…Natural proteins called lectins have powerful antibacterial properties because they bind to carbohydrates on microbial surfaces (Breitenbach et al 2018). Jacalin, a dietary lectin has been used by (Kumar et al 2022) to inhibit the proliferation of cancer cells, they combined Jacalin with the established anti-cancer drug taxol to obtain a better effect on a triple-negative breast cancer line. (Subramaniyan et al 2021) studied Jacalin-derived silver nanoparticles which had good antibacterial activity and it was found to have killed Staphylococcus aureus in less than 30 min, by inducing oxidative stress and membrane damage.…”