Effects of Ischemia and Reperfusion on P2X2 Receptor Expressing Neurons of the Rat Ileum Enteric Nervous System

Paulino, Ariane Silva; Palombit, Kelly; Cavriani, Gabriela; Tavares-de-Lima, Wothan; Mizuno, Márcia Sanae; Marosti, Aline Rosa; Silva, Marcos Vinícius da; Girotti, Priscila Azevedo; Liberti, Édson Aparecido; Castelucci, Patrícia

doi:10.1007/s10620-011-1588-z

Cited by 38 publications

(37 citation statements)

References 53 publications

(53 reference statements)

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“…ATP attenuated intestinal dysfunction produced by ischaemia, but not that caused by reperfusion in rabbits [650]. In ischaemia-reperfusion of the intestine, there was a decrease in P2X2 receptor expression in the myenteric and submucosal plexus [545]. .…”

Section: Ischaemiamentioning

confidence: 88%

Purinergic signalling in the gastrointestinal tract and related organs in health and disease

Burnstock

2013

Purinergic Signalling

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Purinergic signalling plays major roles in the physiology and pathophysiology of digestive organs. Adenosine 5′-triphosphate (ATP), together with nitric oxide and vasoactive intestinal peptide, is a cotransmitter in nonadrenergic, non-cholinergic inhibitory neuromuscular transmission. P2X and P2Y receptors are widely expressed in myenteric and submucous enteric plexuses and participate in sympathetic transmission and neuromodulation involved in enteric reflex activities, as well as influencing gastric and intestinal epithelial secretion and vascular activities. Involvement of purinergic signalling has been identified in a variety of diseases, including inflammatory bowel disease, ischaemia, diabetes and cancer. Purinergic mechanosensory transduction forms the basis of enteric nociception, where ATP released from mucosal epithelial cells by distension activates nociceptive subepithelial primary afferent sensory fibres expressing P2X3 receptors to send messages to the pain centres in the central nervous system via interneurons in the spinal cord. Purinergic signalling is also involved in salivary gland and bile duct secretion.

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Section: Ischaemiamentioning

confidence: 88%

Purinergic signalling in the gastrointestinal tract and related organs in health and disease

Burnstock

2013

Purinergic Signalling

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“…Pretreatment with 59-N-ethylcarboxamidoadenosine, a nonselective A 1 /A 2 agonist, provides partial improvement in intestinal ischemia-reperfusion injury in rats (Özaçmak et al, 2009). Ischemia-reperfusion of the rat intestine causes a decrease in the expression of P2X 2 receptors on neurons of the myenteric and submucosal plexuses, as well as a decrease in density and of neurons immunoreactive for the P2X 2 receptor (Paulino et al, 2011). After intestinal ischemia-reperfusion injury, P2Y 2 receptor mRNA expression was increased in murine lung and kidney and A 3 receptor mRNA was increased in lung but decreased in kidney, but there was no change in mRNA expression of these receptors in the intestine (Milano et al, 2008).…”

Section: Ischemiamentioning

confidence: 99%

Purinergic Signaling and Blood Vessels in Health and Disease

2013

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“…Furness [26] (2006) emphasized that NOS catalyzes the formation of the NO that is present in the myenteric plexus and neuronal processes in the gut, which acts to relax the muscle fiber function of the gastrointestinal musculature. Previous studies have shown the presence of NOS in the enteric ganglia and muscle fibers under various conditions, such as malnutrition and renutrition [27,28] , ischemia and reperfusion [29][30][31] and obesity [32,33] . The enteric neurons of the ICJ stain positive for NOS, PGP 9.5, and c-kit [3,4,6,8] .…”

Section: Semmentioning

confidence: 99%

Effects of aging on the architecture of the ileocecal junction in rats

Brito

Chopard

Cury

et al. 2016

WJGPT

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AIM:To evaluate the structural organization of the elastic and collagen fibers in the region of the ileocecal transition in 30 young and old male Wistar rats. METHODS:Histology, immunohistochemistry (IHC), transmission electron microscopy and scanning electron microscopy were employed in this study. The results demonstrated that there was a demarcation of the ileocecal region between the ileum and the cecum in both groups. RESULTS:The connective tissue fibers had different distribution patterns in the two groups. IHC revealed the presence of nitric oxide synthase, enteric neurons and smooth muscle fibers in the ileocecal junctions (ICJs) of both groups. Compared to the young group, the elderly group exhibited an increase in collagen type Ⅰ fibers, a decrease in collagen type Ⅲ fibers, a decreased linear density of oxytalan elastic fibers, and a greater linear density of elaunin and mature elastic fibers. CONCLUSION:The results revealed changes in the patterns of distribution of collagen and elastic fibers that may lead to a possible decrease in ICJ functionality. Core tip: The ileocolonic sphincter controls the forward and backward flow by integrating its motility with that of the distal ileum and proximal the ileocolonic sphincter controls the forward and backward flow by integrating its motility with that of the distal ileum and proxima. The ileocecal junction (ICJ) includes the muscle bundles in the terminal ileum, the intrinsic nerve plexus. The ICJ includes the muscle bundles in the terminal ileum, the intrinsic nerve plexus. Given the importance of knowing how the ICJ changes with age, the objective of this study was to characterize the morphological changes in the ICJ in rats aged 21 d and 2 years, using optical microscopy and electronic scanning and transmission methodologies. Additionally, the neurochemical characterization of the inhibitory neurons of the myenteric plexus, which are immunoreactive to the enzyme nitric oxide synthase, and the staining of the neuronal population were employed to identify immunoreactivity to HuC/D.de Brito MC, Chopard RP, Cury DP, Watanabe IS, Mendes CE, Castelucci P. Effects of aging on the architecture of the ileocecal junction in rats.

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Effects of Ischemia and Reperfusion on P2X2 Receptor Expressing Neurons of the Rat Ileum Enteric Nervous System

Cited by 38 publications

References 53 publications

Purinergic signalling in the gastrointestinal tract and related organs in health and disease

Purinergic signalling in the gastrointestinal tract and related organs in health and disease

Purinergic Signaling and Blood Vessels in Health and Disease

Effects of aging on the architecture of the ileocecal junction in rats

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