Effects of Isatis root polysaccharide on boar sperm quality during liquid storage and in vitro fertilization

Ren, Zhiqiang; Shaoyong, Weike; Li, Qian; Ma, Lu; Xiao, Junying; Jiao, Junheng; Yang, Gongshe; Pang, Wei

doi:10.1016/j.anireprosci.2019.106178

Cited by 11 publications

(7 citation statements)

References 48 publications

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“…Natural products, such as medicinal plants, could be employed as a cheap alternative to the common antioxidants used in boar semen extenders to reduce the negative effects of high levels of ROS on sperm cells. Recent studies have shown that the addition of plant extracts to extenders enhances sperm function during semen storage and in vitro fertility in boars [12,13].…”

Section: Introductionmentioning

confidence: 99%

Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress

Ros-Santaella

Kadlec

Pintus

2020

Animals

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In recent decades, an increasing number of ethnopharmacological studies have been dedicated to medicinal plants from South African fynbos. Among these plants, honeybush (Cyclopia spp.) has become a popular tea, mainly due to its healthy properties and caffeine-free status. The antioxidant, antimutagenic, and antimicrobial properties of this plant have been reported in several cell types, but its effects on reproductive function are still unknown. Here, we assessed the effects of honeybush (Cyclopia intermedia) on boar sperm parameters under induced oxidative stress (Fe2+/ascorbate) and during five days of semen storage at 17 °C without oxidative stress. In both experiments, four concentrations (200, 50, 12.5, and 3.125 µg/mL) of fermented honeybush were tested. Our results show that honeybush enhances sperm parameters, and no toxic effects were observed at any of the tested extract concentrations. Interestingly, honeybush (12.5 µg/mL) improved the sperm motility and kinetic parameters, preserved the plasma membrane integrity, and reduced the lipid peroxidation in the samples exposed to Fe2+/ascorbate (p < 0.05). In the stored samples, positive effects of honeybush on sperm parameters (motility, kinetics, acrosome, and mitochondria) were observed from 48 h until 120 h of semen storage (p < 0.05). Our results clearly show the protective effects of honeybush on sperm samples, thus promoting its use as a natural source of antioxidants for boar semen.

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Section: Introductionmentioning

confidence: 99%

Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress

Ros-Santaella

Kadlec

Pintus

2020

Animals

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“…Currently, artificial insemination (AI) is employed for sow breeding in over 90% of swine‐producing nations (Waberski, Riesenbeck, et al, 2019). Preserving semen at 17°C is a highly effective technique that allows pig semen to remain viable for up to 5 days, significantly enhancing semen utilization (Jung et al, 2015; Ren et al, 2019). As the preservation time increases, free radicals and reactive oxygen species (ROS) accumulate in the sperm (Aitken, 2017; Tamanini et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Kojic acid inhibits pig sperm apoptosis and improves capacitated sperm state during liquid preservation at 17°C

Hu,

Zhang,

et al. 2024

Molecular Reproduction Devel

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The parameters of sperm apoptosis and capacitation during liquid storage at 17°C can indicate the quality of pig sperm and the potential development of early embryos. However, the effect of kojic acid (KA) on semen preservation and its mechanism has not been fully understood. In this study, we discovered that adding KA to the diluent improved the antioxidant capacity of sperm mitochondria, maintained the normal structure of sperm mitochondria, and reduced sperm apoptosis. Western blot analysis revealed that KA prevented the release of Cytochrome c from mitochondria to the cytoplasm, reduced the expression of pro‐apoptosis proteins cleaved Caspase‐3 and cleaved Caspase‐9, and increased the expression of the antiapoptosis protein Bcl‐XL. Furthermore, KA also enhanced the motility parameters, oxidative phosphorylation level, adenosine triphosphate level, and protein tyrosine phosphorylation of capacitated sperm, while preserving the acrosome integrity and plasma membrane integrity of capacitated sperm. In conclusion, this study offers new insights into the molecular mechanism of how KA inhibits porcine sperm apoptosis and improves capacitated sperm parameters. Additionally, it suggests that KA can serve as an alternative to antibiotics.

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“…Moderate amounts of reactive oxygen species can promote the maturation, capacitation and fertilization processes of sperm, but too much reactive oxygen species can negatively affect sperm, leading to abnormal sperm function or reduced fertilization. The literature has shown that antioxidant supplementation in vitro or in addition to semen diluents in vitro protects sperm from oxidative stress [8][9][10]. However, it may reduce the fertilizing ability of the spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

Supplementing Boar Diet with Nicotinamide Mononucleotide Improves Sperm Quality Probably through the Activation of the SIRT3 Signaling Pathway

Zhang,

Chai,

Cao

et al. 2024

Antioxidants

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Sperm quality is an important indicator to evaluate the reproduction ability of animals. Nicotinamide mononucleotide (NMN) participates in cell energy metabolism and reduces cell oxidative stress. However, the effect and regulatory mechanism of NMN on porcine sperm quality are still unknown. Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality. The results showed that the dietary NMN supplementation significantly increased sperm volume, density and motility (p < 0.05). Interestingly, NMN apparently improved the antioxidative indexes and increased the levels of testosterone (p < 0.05) in serum. Furthermore, NMN upregulated the protein levels of sirtuin 3 (SIRT3), antioxidation and oxidative phosphorylation (OXPHOS), but downregulated the protein levels of apoptosis in semen. Mechanically, NMN protected sperm from H2O2-induced oxidative stress and apoptosis through SIRT3 deacetylation. Importantly, the SIRT3-specific inhibitor 3-TYP attenuated the antioxidation and antiapoptosis of NMN in sperm. Therefore, NMN exerts antioxidation and antiapoptosis to improve boar sperm quality via the SIRT3 signaling pathway. Our findings suggest that NMN is a novel potential boar antioxidative feed additive to produce high-quality porcine semen.

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Effects of Isatis root polysaccharide on boar sperm quality during liquid storage and in vitro fertilization

Cited by 11 publications

References 48 publications

Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress

Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress

Kojic acid inhibits pig sperm apoptosis and improves capacitated sperm state during liquid preservation at 17°C

Supplementing Boar Diet with Nicotinamide Mononucleotide Improves Sperm Quality Probably through the Activation of the SIRT3 Signaling Pathway

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