Cauliflower's (Brassica oleracea var. botrytis L.) fresh appearance and high nutritional values may be related to plant organ water holding and nitrogen (N) translocation from leaf-stem sources to head sinks. Cauliflower plant water and N nutrition relations were examined in a two-site field study in Nova Scotia. The objectives of this study were to assess cauliflower plant organ water and N translocation efficiency and to quantify the correlations between cauliflower plant organ water status, N translocation, head size, and fresh yield under various N treatments applied at shoot-tip-straightened stage. The experimental treatments consisted of three cauliflower hybrid cultivars (cvs. Minuteman, Sevilla, and Whistler) and three N combinations (105 + 30 + 0, 105 + 30 + 45, and 105 + 30 + 90 kg·ha −1 ) supplied at transplanting, seedling standing, and shoot straightened stage, respectively. The N treatments were arranged with four replicates in a split-block design in the two fields. Results showed that cauliflower plant N temporal storage in leaves and stems (source N) peaked (10.6 g/plant) at curd initiation. Plant water index was positively correlated with solar irradiance but negatively correlated with normalized difference vegetative index (NDVI). Nitrogen translocation from leaves and stems to heads was only 5% of total N uptake within the first week of the heading process, but the sink N reached 22.3%-24.9% (or 2.28-2.81 g/plant) of whole plant total N uptake within 15 days at head maturity. Mean N concentrations were significantly higher in heads (5.66%) than in leaves and stems (4.97%) at harvest (P < 0.05). The sink/source N ratios varied between 0.29