Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA and induction of apoptosis in HeLa cells and nasal mucosa of rats

Effects of narrow‐band UVB on nasal symptom and upregulation of histamine H1 receptor mRNA in allergic rhinitis model rats

“…Because sneeze is mediated by nasal trigeminal reflex through H1R, 17 and H1R immunohistochemically localized in the epithelium, vessels and nerves of human nasal mucosa, 18 the present findings suggest that suppression of TDI‐induced increase in amount of H1R in the nasal mucosal nerves by narrow‐band UVB is responsible for the suppression of TDI‐induced sneezes in TDI‐sensitized rats after intranasal irradiation with narrow‐band UVB. Moreover, in our previous in vitro study, narrow‐band UVB suppressed upregulation of both H1R mRNA and protein in human epithelial HeLa cells that endogenously express H1R 16 . Our previous clinical study also showed that levels of H1R mRNA of the nasal mucosa was correlated with the intensity of allergic symptom in patients with AR 8 .…”

“…We reported that the suppression of ERK phosphorylation induced by narrow‐band UVB in the protein kinase C delta (PKCδ)/ERK/poly(ADP‐ribose) polymerase 1 (PARP‐1) signaling pathway is responsible for narrow‐band UVB‐induced inhibition of PMA‐induced upregulation of H1R mRNA in HeLa cells 15,16 . Therefore, it was suggested that the inhibition of TDI‐provoked upregulation of H1R mRNA in the nasal mucosa of TDI‐sensitized rats after intranasal narrow‐band UVB irradiation was also due to its inhibition of ERK phosphorylation.…”

“…It has been reported that DNA damage of human nasal mucosa induced by a single UV irradiation is efficiently removed within a few days, 27 and that failure to properly repair DNA damage may cause gene mutations, finally leading to carcinogenesis 27‐30 . Because low‐dose narrow‐band UVB (600 mJ/cm 2 ) did not induce DNA damage in nasal mucosal cells in rats, intranasal phototherapy with 310 nm narrow‐band UVB at a dose of 600 mJ/cm 2 is safely applicable for the treatment of AR 4,15,16 …”

“…In a previous study, we reported that irradiation with the 310 nm narrow‐band UVB wavelength‐specifically, reversibly, and dose‐dependently suppresses phorbol‐12‐myristate‐13‐acetate (PMA)‐induced upregulation of H1R gene expression in human epithelial HeLa cells 4 . We also reported that irradiation with narrow‐band UVB inhibits phosphorylation of extracellular signal‐regulated kinase (ERK) in the H1R signaling pathway, suggesting that the suppression of ERK phosphorylation is underlying mechanism of the inhibition of PMA‐induced upregulation of H1R gene expression in HeLa cells 15,16 . In this study, we examined whether irradiation with narrow‐band UVB suppressed upregulation of H1R mRNA in vivo.…”

Intranasal Antiviral Drug Delivery and Coronavirus Disease 2019 (COVID‐19): A State of the Art Review

“…57 Animal studies with UV-A and UV-B irradiation demonstrate no histopathologic changes 58 and no induction of apoptosis at lower doses. 59 Other animal studies have shown similar reduction in histopathologic changes with phototherapy as compared with nasal corticosteroid treatment without increasing apoptosis of mucosal cells. 60 UV-C is strongly absorbed by the nucleic acids of microorganisms and therefore is the most lethal range of wavelengths for them.…”