Effects of Irradiation on Epithelial Wound Healing and Microbial Diversity in an in-vitro Oral Mucosa Model

Ryck, Tine De; Boterberg, Tom; Kerckhof, Frederiek‐Maarten; Schrijver, Joachim De; Bracke, Marc; Wiele, Tom Van de; Vanhoecke, Barbara

doi:10.4172/2155-9619.1000218

J Nucl Med Radiat Ther

2014

DOI: 10.4172/2155-9619.1000218

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Effects of Irradiation on Epithelial Wound Healing and Microbial Diversity in an in-vitro Oral Mucosa Model

Tine De Ryck¹,

Tom Boterberg²,

Frederiek‐Maarten Kerckhof³

et al.

Abstract: The impact of irradiation on host-microbe crosstalk is still underexplored. By use of an in vitro oral mucosa model, we show an impact of irradiation on epithelial wound healing depending on the microbial composition and functionality. 454-pyrosequencing analyses pointed to a slight increase in abundance of Rothia, Granulicatella and Gemella in our model after irradiation. Further research is needed to unravel the effects of irradiation on the oral microbiota and the host-microbe interactions more in detail.

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2018

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Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP

Carvalho

Vaz

Pereira

et al. 2018

Sci Rep

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Mucositis is an inflammatory condition of the gut, caused by an adverse effect of chemotherapy drugs, such as 5-fluorouracil (5-FU). In an attempt to develop alternative treatments for the disease, several research groups have proposed the use of probiotics, in particular, Lactic Acid Bacteria (LAB). In this context, the use of recombinant LAB, for delivering anti-inflammatory compounds has also been explored. In previous work, we demonstrated that either Lactococcus lactis NZ9000 or a recombinant strain expressing an antimicrobial peptide involved in human gut homeostasis, the Pancreatitis-associated Protein (PAP), could ameliorate 5-FU-induced mucositis in mice. However, the impact of these strains on the gut microbiota still needs to be elucidated. Therefore, in the present study, we aimed to characterize the effects of both Lactococci strains in the gut microbiome of mice through a 16 S rRNA gene sequencing metagenomic approach. Our data show 5-FU caused a significant decrease in protective bacteria and increase of several bacteria associated with pro-inflammatory traits. The Lactococci strains were shown to reduce several potential opportunistic microbes, while PAP delivery was able to suppress the growth of Enterobacteriaceae during inflammation. We conclude the strain secreting antimicrobial PAP was more effective in the control of 5-FU-dysbiosis.

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Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP

Carvalho

Vaz

Pereira

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

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Effects of Irradiation on Epithelial Wound Healing and Microbial Diversity in an in-vitro Oral Mucosa Model

Cited by 1 publication

References 13 publications

Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP

Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP

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