Effects of iron-oxide nanoparticles and magnetic fields on oral biofilms

Alas, Gema J.; Pagano, Ronald E.; Nguyen, Jane; Bandara, Nihal; Ivanov, Sergei A.; Smolyakov, Gennady A.; Huber, Dale L.; Smyth, Hugh D. C.; Osiński, Marek

doi:10.1117/12.2256221

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…In developing new treatments for dental caries, chemical, microbiological, and microbial-based de- and remineralization models can be used to demonstrate the antimicrobial efficacy or the influence of a treatment on the de- and remineralization process [17,22]. The use of in vitro models has recently demonstrated the potential for novel non-invasive treatment options using preservatives and natural antimicrobial compounds [33], xylitol [26], probiotics [94,95,96,97], hydrogen peroxide [98], and nanoparticles [99,100,101]. While the discovery of these potential treatments is promising, the development of a simple, yet robust, high-throughput screening model system is needed for the rapid identification and optimization of novel treatments that can demonstrate the correction of oral microbiome dysbiosis.…”

Section: Discussionmentioning

confidence: 99%

A Multispecies Biofilm In Vitro Screening Model of Dental Caries for High-Throughput Susceptibility Testing

Heersema

2019

High-Throughput

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There is a current need to develop and optimize new therapeutics for the treatment of dental caries, but these efforts are limited by the relatively low throughput of relevant in vitro models. The aim of this work was to bridge the 96-well microtiter plate system with a relevant multispecies dental caries model that could be reproducibly grown to allow for the high-throughput screening of anti-biofilm therapies. Various media and inoculum concentrations were assessed using metabolic activity, biomass, viability, and acidity assays to determine the optimal laboratory-controlled conditions for a multispecies biofilm composed of Streptococcus gordonii, Streptococcus mutans, and Candida albicans. The selected model encompasses several of the known fundamental characteristics of dental caries-associated biofilms. The 1:1 RPMI:TSBYE 0.6% media supported the viability and biomass production of mono- and multispecies biofilms best. Kinetic studies over 48 h in 1:1 RPMI:TSBYE 0.6% demonstrated a stable biofilm phase between 10 and 48 h for all mono- and multispecies biofilms. The 1:1:0.1 S. gordonii: S. mutans: C. albicans multispecies biofilm in 1:1 RPMI:TSBYE 0.6% is an excellent choice for a high-throughput multispecies model of dental caries. This high-throughput multispecies model can be used for screening novel therapies and for better understanding the treatment effects on biofilm interactions and stability.

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Section: Discussionmentioning

confidence: 99%

A Multispecies Biofilm In Vitro Screening Model of Dental Caries for High-Throughput Susceptibility Testing

Heersema

2019

High-Throughput

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“…The application of an external magnetic field (MF) to an infected area enhances the biofilm disruption action of IONPs. We previously reported on the antimicrobial effects of IONPs and enhanced effects with external magnetic field application [4,9,10]. Herein we present a snapshot of a work-in-progress in which we aim to optimize this system for use as a dental antimicrobial by investigating IONPs of different sizes, comparing Fe2O3 (red iron oxide) to Fe3O4 (black iron oxide), and comparing dextran with alginate as coating materials.…”

Section: Introductionmentioning

confidence: 99%

Superparamagnetic nanoparticles for the treatment of periodontal disease

Fritz¹,

Armijo²,

Simpson³

et al. 2023

Colloidal Nanoparticles for Biomedical Applications XVIII

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Periodontal diseases are prevalent worldwide and are linked to numerous other health conditions due to dysbiosis and chronic inflammatory state. Most periodontal diseases are caused by pathogenic bacteria that colonize dental tissues in the form of biofilm. Eradication of bacterial biofilms can be difficult to achieve due to the complex architecture of the teeth and gums which complicates the removal. Orthodontic wires and dental devices introduce additional hurdles to the adequate removal of biofilms by traditional methods since mechanical disruption via direct contact with toothbrush bristles, floss, and abrasive toothpaste is limited. Magnetically activated nanoparticles (NPs), specifically iron oxide nanoparticles (IONPs) that can be functionalized as antimicrobial particles and remotely controlled by magnetic fields, are of interest for oral biofilm eradication. We present data in multi-species bacterial cultures, established biofilms, human gingival keratinocytes, and human gingival fibroblast cells alone and in the presence of multispecies biofilm co-cultures to determine the safest, most efficacious IONP size ranges and treatment concentrations of active magnetic NPs for removal of dental biofilms. We report enhanced efficacy for IONPs coated with alginate vs. dextran, and small sizes (~8 nm vs. >20 nm in size) appear to exhibit enhanced antimicrobial efficacy. Human gingival keratinocyte (TIGK) cells in co-culture with treated and untreated multispecies biofilms in an in-vitro periodontitis model also exhibited a trend of reduced inflammatory markers in wells with IONP-treated biofilms.

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Effects of iron-oxide nanoparticles and magnetic fields on oral biofilms

Cited by 2 publications

References 25 publications

A Multispecies Biofilm In Vitro Screening Model of Dental Caries for High-Throughput Susceptibility Testing

A Multispecies Biofilm In Vitro Screening Model of Dental Caries for High-Throughput Susceptibility Testing

Superparamagnetic nanoparticles for the treatment of periodontal disease

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