Effects of iron oxide nanoparticles on cardiac differentiation of embryonic stem cells

Au, Ka-Wing; Liao, Song-Yan; Lee, Yee-Ki; Lai, Wing‐Hon; Ng, Kwong‐Man; Chan, Yau-Chi; Yip, Mei-Chu; Ho, Chung-Yee Jenny; Wu, Ed X.; Li, Ronald A; Siu, Chung‐Wah; Tse, Hung‐Fat

doi:10.1016/j.bbrc.2008.12.160

Cited by 68 publications

(55 citation statements)

References 21 publications

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“…1,3,39,40 However, current knowledge is still unsystematic and restricted to particular aspects of nanoparticles' capability to induce toxic and pathological processes or to alter structure and function in different cell types. 41À43 Nevertheless, among nanoparticles, ceria is redox-active and has shown potential for antioxidant therapies controlling ROS levels.…”

Section: Discussionmentioning

confidence: 99%

Cerium Oxide Nanoparticles Protect Cardiac Progenitor Cells from Oxidative Stress

et al. 2012

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Cardiac progenitor cells (CPCs) are a promising autologous source of cells for cardiac\ud regenerative medicine. However, CPC culture in vitro requires the presence of microenvironmental\ud conditions (a complex array of bioactive substance concentration, mechanostructural\ud factors, and physicochemical factors) closely mimicking the natural cell surrounding in vivo,\ud including the capability to uphold reactive oxygen species (ROS) within physiological levels\ud in vitro. Cerium oxide nanoparticles (nanoceria) are redox-active and could represent a potent\ud tool to control the oxidative stress in isolated CPCs. Here, we report that 24 h exposure to 5, 10,\ud and 50 !g/mL of nanoceria did not a!ect cell growth and function in cardiac progenitor cells,\ud while being able to protect CPCs from H2O2-induced cytotoxicity for at least 7 days, indicating\ud that nanoceria in an e!ective antioxidant. Therefore, these "ndings con"rm the great\ud potential of nanoceria for controlling ROS-induced cell damage

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Section: Discussionmentioning

confidence: 99%

Cerium Oxide Nanoparticles Protect Cardiac Progenitor Cells from Oxidative Stress

et al. 2012

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“…Murine embryonic stem cell culture and in vitro cardiac differen tiation Murine (m) ES cell-line D3 (CRL-1934, American Type Culture Collection, Manassas, VA) was used and cultured as previously described [21] . Briefly, undifferentiated mESCs were cultured on an irradiation-inactivated monolayer of mouse embryonic fibroblast feeders in Dulbecco's modified Eagle's minimal essential medium (DMEM, Gibco BRL, Karlsruhe, Germany), supplemented with 15% fetal bovine serum (FBS, Gibco BRL, Karlsruhe, Germany), 0.1 mmol/L mercaptoethanol (Sigma-Aldrich, St Louis, MO), non-essential amino acids (stock solution diluted 1:100; Hyclone, Logan, UT) and 1000 U/mL of recombinant mouse leukemia inhibitory factor (LIF) (Chemicon, Hofheim, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, undifferentiated mESCs were cultured on an irradiation-inactivated monolayer of mouse embryonic fibroblast feeders in Dulbecco's modified Eagle's minimal essential medium (DMEM, Gibco BRL, Karlsruhe, Germany), supplemented with 15% fetal bovine serum (FBS, Gibco BRL, Karlsruhe, Germany), 0.1 mmol/L mercaptoethanol (Sigma-Aldrich, St Louis, MO), non-essential amino acids (stock solution diluted 1:100; Hyclone, Logan, UT) and 1000 U/mL of recombinant mouse leukemia inhibitory factor (LIF) (Chemicon, Hofheim, Germany). To induce cardiac differentiation, embryoid bodies (EBs) were generated from hanging drops of approximately 800 mESCs in 20 μl of culture medium in the absence of leukemia inhibitory factor and feeder cells for two days and then grown in suspension or five more days [21] . …”

Section: Methodsmentioning

confidence: 99%

Ouabain facilitates cardiac differentiation of mouse embryonic stem cells through ERK1/2 pathway

Lee

Lai

et al. 2010

Acta Pharmacol Sin

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Aim: To investigate the effects of the cardiotonic steroid, ouabain, on cardiac differentiation of murine embyronic stem cells (mESCs). Methods: Cardiac differentiation of murine ESCs was enhanced by standard hanging drop method in the presence of ouabain (20 µmol/L) for 7 d. The dissociated ES derived cardiomyocytes were examined by flow cytometry, RT-PCR and confocal calcium imaging. Results: Compared with control, mESCs treated with ouabain (20 µmol/L) yielded a significantly higher percentage of cardiomyocytes, and significantly increased expression of a panel of cardiac markers including Nkx 2.5, α-MHC, and β-MHC. The α1 and 2-isoforms Na + /K + -ATPase, on which ouabain acted, were also increased in mESCs during differentiation. Among the three MAPKs involved in the cardiac hypertrophy pathway, ouabain enhanced ERK1/2 activation. Blockage of the Erk1/2 pathway by U0126 (10 µmol/L) inhibited cardiac differentiation while ouabain (20 µmol/L) rescued the effect. Interestingly, the expression of calcium handling proteins, including ryanodine receptor (RyR2) and sacroplasmic recticulum Ca 2+ ATPase (SERCA2a) was also upregulated in ouabain-treated mESCs. ESC-derived cardiomyocyes (CM) treated with ouabain appeared to have more mature calcium handling. As demonstrated by confocal Ca 2+ imaging, cardiomyocytes isolated from ouabain-treated mESCs exhibited higher maximum upstroke velocity (P<0.01) and maximum decay velocity (P<0.05), as well as a higher amplitude of caffeine induced Ca 2+ transient (P<0.05), suggesting more mature sarcoplasmic reticulum (SR). Conclusion: Ouabain induces cardiac differentiation and maturation of mESC-derived cardiomyocytes via activation of Erk1/2 and more mature SR for calcium handling.

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“…The first application of SPIO in cardiovascular regenerative medicine therapies was to label exogenous stem cells in order track these cells after injection into the infarct region in rodent models of MI (Kraitchman et al, 2003). While several studies show that IONPs do not affect cell viability, phenotype or therapeutic potential (Au et al, 2009;Li et al, 2013), other studies have raised concerns on their safety (Mahmoudi et al, 2011(Mahmoudi et al, , 2012Ge et al, 2013). Furthermore, their ability to track transplanted cells by MRI is controversial as the uptake of IONP by resident macrophages in the advent of transplanted cell apoptosis results in false-positive signals of cell engraftment in the infarct (Amsalem et al, 2007).…”

Section: (Vlp Vaccine Formentioning

confidence: 99%

Nano-Engineered Biomaterials for Tissue Regeneration: What Has Been Achieved So Far?

et al. 2016

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Nanomaterials have attracted the interest of tissue engineers for the last two decades. Their unique properties make them promising for de novo fabrication of bio-inspired hybrid/composite materials with improved regenerative properties, including, for example, the capacity for electric conductivity and the provision of antimicrobial properties. However, to this day, the use of such materials in medical applications is rather limited and most of the studies have only reached the archetypical proof-of-concept stage. Herein, we present a review on the use of nanomaterials in tissue engineering for regenerative therapies of heart, skin, eye, skeletal muscle, and nervous system. The advantages and limitations of nano-engineering materials are presented in this review alongside with the future challenges and milestones nanotechnology must overcome to make an impact in biomedical applications.

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Effects of iron oxide nanoparticles on cardiac differentiation of embryonic stem cells

Cited by 68 publications

References 21 publications

Cerium Oxide Nanoparticles Protect Cardiac Progenitor Cells from Oxidative Stress

Cerium Oxide Nanoparticles Protect Cardiac Progenitor Cells from Oxidative Stress

Ouabain facilitates cardiac differentiation of mouse embryonic stem cells through ERK1/2 pathway

Nano-Engineered Biomaterials for Tissue Regeneration: What Has Been Achieved So Far?

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