Effects of Iron Ions on Rosmarinic Acid Production and Antioxidant System in Melissa officinalis L. Seedlings

Abstract

This work was carried out in collaboration between both authors. Author KES performed the statistical analysis, wrote the protocol, and wrote the first draft of the manuscript. Author ARM designed the study, managed the analyses of the study. Both authors read and approved the final manuscript.

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

“…These anti‐oxidative enzymes have a role in H 2 O 2 elimination . Iron is a main component of oxidation–reduction systems such as heme proteins, including cytochromes, catalase, peroxidase, and leghemoglobin and iron sulfur proteins, including ferredoxin, aconitase, and superoxide dismutase . The GPX and CAT enzymes contain iron porphyrin; thus their activities are probably affected by iron concentrations .…”

“…71 Iron is a main component of oxidation-reduction systems such as heme proteins, including cytochromes, catalase, peroxidase, and leghemoglobin and iron sulfur proteins, including ferredoxin, aconitase, and superoxide dismutase. 35 The GPX and CAT enzymes contain iron porphyrin; thus their activities are probably affected by iron concentrations. 72 According to reports by Moharrami et al 28 , Fe NPs with enhancement of CAT and GPX activity in H. reticulatus hairy-root cultures, reduced oxidative damage, whereas APX activity was not changed significantly by Fe NPs.…”

“…32 Due to the importance of the pharmaceutical and industrial applications of RA, its production has been studied in various plant species (Orthosiphon aristatus, Salvia miltiorrhiza, and Melissa officinalis) influenced by biological and non-biological elicitors. [33][34][35] In Agastache foeniculum hairy root cultures, a fourfold increase in RA content was observed. 36 The production of RA was elevated threefold in D. moldavicac hairy root culture.…”

“…The amount of saponin content in Calendula officinalis increased compared to the control roots after treatment with 0.4 mM nanosilver . Due to the importance of the pharmaceutical and industrial applications of RA, its production has been studied in various plant species ( Orthosiphon aristatus , Salvia miltiorrhiza , and Melissa officinalis) influenced by biological and non‐biological elicitors . In Agastache foeniculum hairy root cultures, a fourfold increase in RA content was observed .…”

COPPER ION INDUCED PRODUCTION OF ROSMARINIC ACID IN LEMON BALM (Melissa officinalis L.) SEEDLINGS

“…В возрасте 45 сут проростки извлекали из агаризованной среды, тщательно промывали стерильной дистиллированной водой и переносили в жидкую среду MS. В среду добавляли Cu 2+ до конечных концентраций 0 (контроль), 5, 10, 20 и 30 мкМ (использовали соль CuSO 4 •5H2O. Как и в предыдущей нашей работе, обработка продолжалась в течение 8 и 16 ч (27). Обработанные проростки собирали, несколько раз промывали стерильной дистиллированной водой для удаления поверхностных ионов и разделяли на две части: одну сушили в тени (в течение 3 сут) и использовали для измерения концентрации RA, другую ополаскивали, обезвоживали, замораживали в жидком азоте и хранили при 80 С для последующего измерения содержания флавоноидов и антоцианов, активности антиоксидантных ферментов и анализа уровня экспрессии гена ТАТ.…”